Core B

核心B

基本信息

批准号：
10189640
负责人：
Heather A Drummond
金额：
$ 96.37万
依托单位：
UNIVERSITY OF MISSISSIPPI MED CTR
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-06-08 至 2022-08-04
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10189640
关键词：
Back Basic Science Bioinformatics Biological Assay Biometry Blood Pressure Body Temperature Businesses Cells Centers of Research Excellence Charge Childhood Chronic Clinical Research Consanguinity Data Data Analyses Development Echocardiography Enzyme-Linked Immunosorbent Assay Equipment Faculty Fees Fluorescence-Activated Cell Sorting Fostering Funding Genomics Goals Gonadal Steroid Hormones Grant Guidelines Heart Rate Histology Human Resources Image Immune Maintenance Measurement Measures Metabolic Diseases Mississippi Modeling Morphology Neonatal Neurosciences Perinatal Population Research Research Personnel Resources Rodent Services Students Supervision T-Lymphocyte Technical Expertise Telemetry Training Ultrasonography Universities Wages X-Ray Computed Tomography analytical tool equipment acquisition fetal genetic analysis genetic pedigree genomic data improved liquid chromatography mass spectroscopy macrophage novel programs ranpirnase

项目摘要

MISSISSIPPI CENTER OF EXCELLENCE IN PERINATAL RESEARCH (CEPR) RESEARCH RESOURCES CORE B SUMMARY The goal of the Research Resources Core B of the CEPR is to provide COBRE investigators and trainees with access to state-of-the-art equipment and technical expertise needed to perform their research. New Sub-cores that will be developed in this COBRE include a Pediatric Echocardiography (ECHO) Sub-core, a Telemetry Sub-Core, and a Fluorescence-Activated Cell Sorting (FACS) Sub-Core. There will also be a Biostatistics and Bioinformatics Sub-Core in Core B since there will be considerable genomic data that will need to be analyzed for Project IV and there is no capacity for this in the other COBREs. These sub-cores are novel and are not funded by the existing COBREs, the Cardiorenal and Metabolic Diseases Research COBRE (P20 GM104357) (CMDR) or the Center for Psychiatric Neurosciences COBRE (P30 GM103328) or the University of Southern Mississippi INBRE program. Additional resources will be put into existing CMDR COBRE Sub-Cores for use by the CEPR investigators, the Bioanalytical Sub-Core, the Genomics Core and the Histology and Imaging Sub- Core. The Specific Aims of the Research Resources Core B include the following: 1) To provide services that foster cutting edge clinical and basic research of the highest quality. 2) To provide a Pediatric Echocardiography Sub-Core that can perform both neonatal and fetal sonography. 3) To provide a centrally located, well-equipped Telemetry Sub-Core for the chronic measurement of blood pressure, heart rate, activity and body temperature in rodents. 4) To provide a well equipped Fluorescence-Activated Cell Sorting Sub-Core that can be used to separate immune cells, such as populations of T cells, macrophages, etc, into groups for analysis and quantification. 5) To provide Biostatistics and bioinformatics analyses of data collected in the Projects and by the Sub-Cores. 6) To provide maintenance and supervision of key equipment and provide training, technical support, and supervision for all investigators, staff and students in the proper utilization of Core B equipment. 7) To provide salary and supply support for the CMDR Genomics Sub Core Director to perform genomic analyses for consanguinity for Project IV. 8) To provide salary and supply support for the CMDR Liquid Chromatography/Mass Spectroscopy Sub-Core to develop and measure sex steroids for Project II and III. 9) To provide supply support for the CMDR Bioanalytical Subcore so as to allow the CEPR COBRE investigators to have access to cutting edge RIA and ELISA assays necessary for completion of their Projects I-III. 10) To provide supply support for the CMDR Histology and Imaging Sub-Core so junior investigators have access to state-of-the-art imaging (specifically, computed tomography), and histology and morphology. 11) To optimize all assays and core services, to provide improvement in existing assays and services, and to assist in the interpretation of results and data. 12) To develop a business model for Core B that is compliant with federal funding guidelines to provide support for sustainability of Core B services, equipment, and facilities. The services of Core B are absolutely necessary for the successful completion of the studies described in Projects I-IV and will be an asset to Pipeline grant investigators. Furthermore, any other UMMC investigators will have access to the Core B services on a charge-back fee basis.

密西西比州围产期研究卓越中心 (CEPR) 研究资源核心 B 概括 CEPR 研究资源核心 B 的目标是为 COBRE 研究人员和学员提供获得进行研究所需的最先进的设备和技术专业知识。新的子核心将在此 COBRE 中开发的功能包括儿科超声心动图 (ECHO) 子核心、遥测子核心和荧光激活细胞分选 (FACS) 子核心。还将有生物统计学和核心 B 中的生物信息学子核心，因为需要分析大量基因组数据对于项目 IV，其他 COBRE 没有能力进行此操作。这些子核心很新颖，但并不由现有 COBRE、心肾和代谢疾病研究 COBRE (P20 GM104357) 资助 (CMDR) 或 COBRE 精神神经科学中心 (P30 GM103328) 或南方大学密西西比州 INBRE 计划。额外的资源将投入到现有的 CMDR COBRE 子核心中，以供以下人员使用： CEPR 研究人员、生物分析子核心、基因组学核心以及组织学和成像子核心核。研究资源核心 B 的具体目标包括以下内容： 1) 提供促进最高质量的前沿临床和基础研究的服务。 2) 提供可进行新生儿和胎儿超声检查的儿科超声心动图子核心。 3) 提供位于中心、设备齐全的遥测子核心，用于血液的长期测量啮齿类动物的压力、心率、活动和体温。 4) 提供装备精良的荧光激活细胞分选子核，可用于分离将免疫细胞（例如 T 细胞群、巨噬细胞等）分组进行分析和定量。 5) 对项目和子核心收集的数据进行生物统计学和生物信息学分析。 6) 提供关键设备的维护和监督，并提供培训、技术支持和服务监督所有研究者、工作人员和学生正确使用 Core B 设备。 7) 为CMDR基因组学副核心主任进行基因组学工作提供薪资和物资支持项目 IV 的血缘关系分析。 8) 为CMDR液相色谱/质谱子核心提供薪资和物资支持为项目 II 和 III 开发和测量性类固醇。 9) 为CMDR生物分析子核心提供供应支持，以便CEPR COBRE 研究人员能够获得完成项目所需的最先进的 RIA 和 ELISA 检测方法一-三。 10) 为 CMDR 组织学和成像子核心提供供应支持，以便初级研究人员获得最先进的成像（特别是计算机断层扫描）以及组织学和形态学。 11) 优化所有检测和核心服务，改进现有检测和服务，并协助解释结果和数据。 12) 为核心 B 开发一个符合联邦资助指南的商业模式，以提供支持核心 B 服务、设备和设施的可持续性。核心 B 的服务对于成功完成中所述的研究是绝对必要的项目 I-IV 将成为管道资助研究人员的资产。此外，任何其他 UMMC 调查员将可以在收取退款费用的基础上使用核心 B 服务。