Evaluating Efficacy of Antiseptic Mouth Rinses on Salivary SARS-CoV-2 Infectivity

评估抗菌漱口水对唾液 SARS-CoV-2 感染性的功效

• 批准号: 10354566
• 负责人: Laura Anne Jacox
• 金额: $ 15.55万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-03-10 至 2024-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10354566
• 关键词: 2019-nCoV; Address; Adoption; Aerosols; Antibodies; Antibody Response; Antibody titer measurement; Antigenic Specificity; Biological Assay; COVID-19; COVID-19 pandemic; COVID-19 patient; Caring; Cessation of life; Cetylpyridinium Chloride; Chlorhexidine; Clinical Trials; Collaborations; Critical Illness; Data; Dental; Dentists; Development; Discipline; Disease; Ensure; Ethanol; Evaluation; FDA approved; Face; Faculty; Frontline worker; Generations; Goals; Head and neck structure; Health Personnel; Healthcare; Hydrogen Peroxide; Immune response; Immunoglobulin A; Immunoglobulin G; Immunoglobulin M; Immunologics; Infection; Inflammation; Inflammatory Response; Intervention; Investigation; Knowledge; Local Anti-Infective Agents; Maps; Measures; Mediator of activation protein; Methodology; Mouthwash; Occupational Exposure; Oral; Oral Characters; Oral cavity; Outcome; Outpatients; Patients; Population; Povidone-Iodine; Prognostic Marker; Property; Proteins; Protocols documentation; Public Health; Randomized Clinical Trials; Randomized Controlled Trials; Reporting; Reverse Transcriptase Polymerase Chain Reaction; SARS-CoV-2 infection; Saliva; Salivary; Salivary Glands; Sampling; School Dentistry; Site; Social Environment; Technology; Time; Ultrasonics; Vaccines; Viral; Viral Load result; Virus Replication; Work; Workplace; anti-viral efficacy; biobank; chemokine; cohort; cytokine; diagnostic biomarker; efficacy evaluation; experience; health care settings; high risk; in vivo; infection risk; innovation; novel; novel diagnostics; pathogen; professional atmosphere; public health emergency; respiratory; saliva sample; social; socioeconomics; therapeutic biomarker; tool; translational impact; transmission process; viral transmission; virus envelope; 2019-nCoV; Address; Adoption; Aerosols; Antibodies; Antibody Response; Antibody titer measurement; Antigenic Specificity; Biological Assay; COVID-19; COVID-19 pandemic; COVID-19 patient; Caring; Cessation of life; Cetylpyridinium Chloride; Chlorhexidine; Clinical Trials; Collaborations; Critical Illness; Data; Dental; Dentists; Development; Discipline; Disease; Ensure; Ethanol; Evaluation; FDA approved; Face; Faculty; Frontline worker; Generations; Goals; Head and neck structure; Health Personnel; Healthcare; Hydrogen Peroxide; Immune response; Immunoglobulin A; Immunoglobulin G; Immunoglobulin M; Immunologics; Infection; Inflammation; Inflammatory Response; Intervention; Investigation; Knowledge; Local Anti-Infective Agents; Maps; Measures; Mediator of activation protein; Methodology; Mouthwash; Occupational Exposure; Oral; Oral Characters; Oral cavity; Outcome; Outpatients; Patients; Population; Povidone-Iodine; Prognostic Marker; Property; Proteins; Protocols documentation; Public Health; Randomized Clinical Trials; Randomized Controlled Trials; Reporting; Reverse Transcriptase Polymerase Chain Reaction; SARS-CoV-2 infection; Saliva; Salivary; Salivary Glands; Sampling; School Dentistry; Site; Social Environment; Technology; Time; Ultrasonics; Vaccines; Viral; Viral Load result; Virus Replication; Work; Workplace; anti-viral efficacy; biobank; chemokine; cohort; cytokine; diagnostic biomarker; efficacy evaluation; experience; health care settings; high risk; in vivo; infection risk; innovation; novel; novel diagnostics; pathogen; professional atmosphere; public health emergency; respiratory; saliva sample; social; socioeconomics; therapeutic biomarker; tool; translational impact; transmission process; viral transmission; virus envelope

ABSTRACT Evaluating the antiviral potential of widely available commercial antiseptic rinses on SARS-CoV-2 infectivity holds great potential for rapidly reducing viral transmission, particularly as novel strains emerge. Given that SARS- CoV-2 is transmitted primarily through aerosols and salivary and respiratory secretions, strategies to reduce viral burden is an attractive approach to mitigating spread; this is particularly true for dental and other healthcare settings which have high patient volumes, require work in close proximity to the head and neck, and generate procedural aerosols. Though studies have demonstrated that some antiseptic mouth rinses have antiviral properties against SARS-CoV-2 in vitro and other enveloped viruses in vivo, data are still emerging from recent randomized clinical trials (RCT), including ours at the Adams School of Dentistry, regarding whether antiseptic mouth rinses reduce SARS-CoV-2 in vivo. To address this gap in knowledge, we propose to determine whether mouth rinses containing ethanol, hydrogen peroxide, povidone-iodine, chlorhexidine, or cetylpyridinium chloride reduce SARS-CoV-2 viral load, replication and infectivity (Aim 1) and to characterize salivary inflammatory and antibody responses associated with early SARS-CoV-2 infection (Aim 2) using salivary samples derived from our recent COVID-19+ patient RCT. Altogether, the data generated from these aims will demonstrate whether antiseptic rinses reduce salivary SARS-CoV-2 viral load and infectivity, and will characterize the local inflammatory response to SARS-CoV-2 in the oral cavity with the ultimate goal of limiting viral spread. These studies will generate data to develop feasible, inexpensive, rapidly implementable protocols to mitigate the spread of SARS-CoV-2 and address this urgent public health need with broad translational impact. Our interdisciplinary team is uniquely qualified to conduct the proposed work as we have the innovative methodologies, BLS2+ facilities, SARS-CoV-2+ samples, and expertise to achieve our aims. This application includes collaboration between experienced and junior faculty across multiple disciplines to ensure the rigor and translatability of our findings.

抽象的 评估SARS-COV-2感染性上广泛可用的商业防腐冲洗的抗病毒潜力 快速降低病毒传播的巨大潜力，尤其是随着新型菌株的出现。考虑到那个sars- COV-2主要通过气溶胶，唾液和呼吸道分泌物传播，降低病毒的策略 负担是一种减轻传播的有吸引力的方法。对于牙科和其他医疗保健尤其如此 患者量较高的设置需要靠近头部和颈部的工作，并产生 程序气溶胶。尽管研究表明某些杀菌性嘴里有抗病毒 在体内针对体外和其他包膜病毒的SARS-COV-2的特性，数据仍来自最近 随机临床试验（RCT），包括我们在亚当斯牙科学院的包括抗菌性 口腔冲洗量减少体内SARS-COV-2。为了解决知识的这一差距，我们建议确定是否 口腔冲洗含有乙醇，过氧化氢，普韦酮 - 碘，氯己定或氯丁吡啶二吡啶丁烷 降低SARS-COV-2病毒负荷，复制和感染性（AIM 1），并表征唾液炎症和 使用从唾液样本中得出的唾液样本，与早期SARS-COV-2感染相关的抗体反应（AIM 2） 我们最近的Covid-19+患者RCT。总共，从这些目标产生的数据将证明是否是否 防腐冲洗症降低唾液SARS-COV-2病毒载荷和感染性，并将表征局部 口腔中对SARS-COV-2的炎症反应，其最终目的是限制病毒蔓延。这些 研究将生成数据以开发可行，廉价，可快速实现的协议，以减轻 SARS-COV-2的传播并满足这种紧急的公共卫生需求，并具有广泛的翻译影响。我们的 跨学科团队具有独特的资格来进行拟议的工作，因为我们具有创新性 方法论，BLS2+设施，SARS-COV-2+样本和专业知识，以实现我们的目标。此应用程序 包括在多个学科的经验和初级教师之间的合作，以确保严格和 我们发现的翻译性。