CryoEM Data Collection Facility Consortium at NCM

NCM 的 CryoEM 数据收集设施联盟

• 批准号: 9975875
• 负责人: ESTHER BULLITT
• 金额: $ 51.56万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9975875
• 关键词: Academic Training; Advisory Committees; Arbitration; Biological; Boston; Cells; Conflict (Psychology); Conflict of Interest; Critiques; Cryo-electron tomography; Cryoelectron Microscopy; Data; Data Collection; Data Storage and Retrieval; Department chair; Detection; Development; Devices; Distant; Educational workshop; Electron Microscope; Electrons; Environment; Funding; Image; Imaging technology; Infrastructure; Institution; Laboratories; Lead; Logistics; Mammalian Cell; Medical; Medical center; Medicine; Microscope; Mission; Missouri; Molecular Machines; Noise; Outcome; Participant; Persons; Phase; Policies; Productivity; Proteins; Protocols documentation; Readiness; Recommendation; Request for Applications; Research; Research Personnel; Resolution; Resource Allocation; Resources; Sampling; Schedule; Scientist; Services; Signal Transduction; Specialist; Specimen; Strategic Planning; Structure; Texas; Time; Training; Training Activity; Training Programs; Travel; United States National Institutes of Health; Universities; Virginia; Virus; Visit; base; cellular imaging; chaperonin; college; contrast imaging; cost; data acquisition; data exchange; data management; detector; electron tomography; experience; field emission gun; flexibility; image processing; improved; instrument; instrumentation; macromolecule; meetings; member; movie; operation; particle; public health relevance; structural biology; symposium; technology development

 DESCRIPTION (provided by applicant): The National Center for Macromolecular Imaging (NCMI) at Baylor College of Medicine is the host laboratory offering to provide a portion of the time on their JEM3200FSC and JEM2200FS electron microscopes for a cryo-electron microscopy (cryoEM) and tomography (cryoET) data acquisition facility Consortium. Both instruments are equipped with a field emission gun and an in-column energy filter, while the JEM2200FS also has a Zernike phase plate attachment. Both instruments are currently equipped with DE20 and DE12 cameras operated in integrating mode. These microscopes have been used productively for a variety of biological specimens, including macromolecules, molecular machines and cells, at state-of-the-art resolution. Our facilities will be allocated for he proposed Consortium while taking into account the ongoing research in the host institution. Our lead PI, Wah Chiu is a recognized expert in pushing cryoEM and cryoET beyond current boundaries and has decades of experience in leading several NIH funded research centers and academic training programs. There are 11 participating institutions across the USA, led by investigators who have a research track record in cryoEM and/or cryoET. Day-to-day operation will be carried out by a part-time cryoEM/ET scientist and IT staff. Both in person and remote data collection protocols will be used for distant users. The Consortium will provide travel funds for the distant users. We request funding for a Direct Detection Device (DDD) operated in counting mode to meet the needs of all types of specimens. The host Institution will provide an administrative assistant to support user visits and various Consortium activities, and matching funds to purchase the DDD and adequate hard drives for short-term data storage. The participating PIs will be involved in formulating the specific policies for Consortium governance and developing training activities. Scheduling will be allocated evenly in the first year among participants with some flexibility based on specimen readiness. Allocation of microscope times and priorities will be assigned in the second year and beyond based on recommendations of an external microscope allocation committee appointed by all the PIs to avoid conflict of interest. Al PIs will meet quarterly on WebEx to discuss technical and administrative issues, and we will hold an annual user meeting. An annual review of all aspects of the Consortium operation and the data outcomes of the users will be conducted by an advisory committee to assure the highest productivity and usage of the proposed facility. The membership of the participating institutions will be dynamically reviewed by all the PIs with the inputs from the external advisory committee after the second year of our operation.

 描述（由申请人提供）：贝勒医学院国家大分子成像中心 (NCMI) 是主实验室，提供部分时间使用 JEM3200FSC 和 JEM2200FS 电子显微镜进行冷冻电子显微镜 (cryoEM) 和断层扫描（cryoET）数据采集设施联盟这两种仪器都配备了场发射枪和柱内能量过滤器。 JEM2200FS 还配有 Zernike 相板附件，这两种仪器目前都配备了在积分模式下运行的 DE20 和 DE12 相机，这些显微镜已有效地用于各种生物样本，包括大分子、分子机器和细胞。 -最先进的决议。我们的设施将被分配给他提议的联盟，同时考虑到我们的首席 PI，Wah Chiu 是推动冷冻电镜和冷冻电子治疗的公认专家。超越目前的界限，在领导多个 NIH 资助的研究中心和学术培训项目方面拥有数十年的经验。美国有 11 个参与机构，由在冷冻电镜和/或冷冻电子治疗方面拥有研究记录的研究人员领导。操作将由兼职冷冻EM/ET 科学家和IT 人员进行，该联盟将为远程用户提供亲自和远程数据收集协议。检测装置（DDD）以计数模式运行，以满足各类标本的需求。主办机构将提供行政助理支持用户访问和各种联盟活动，并配套资金购买DDD和足够的短期数据硬盘。参与的 PI 将参与制定联盟治理的具体政策，并在第一年在参与者之间均匀分配培训活动，并根据标本准备情况进行一定的灵活性分配。第二年根据所有 PI 任命的外部显微镜分配委员会的建议，以避免利益冲突，Al PI 将每季度在 WebEx 上开会讨论技术和管理问题，并且我们将举行年度用户会议。联盟运营和用户数据结果的各个方面将由咨询委员会负责，以确保拟议设施的最高生产力和使用率，所有 PI 将根据联盟的意见动态审查参与机构的成员资格。外部咨询 我们运营第二年后的委员会。

项目成果

Structural insights into species-specific features of the ribosome from the human pathogen Mycobacterium tuberculosis.

• DOI: 10.1093/nar/gkx785
• 发表时间: 2017-10-13
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Yang K;Chang JY;Cui Z;Li X;Meng R;Duan L;Thongchol J;Jakana J;Huwe CM;Sacchettini JC;Zhang J
• 通讯作者: Zhang J