Tick Resources Core

勾选资源核心

• 批准号: 9976331
• 负责人: Ulrike Gertrud Munderloh
• 金额: $ 19.25万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-13 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9976331
• 关键词: Adult; American Type Culture Collection; Anaplasma phagocytophilum; Animal Model; Arthropods; Basic Science; Biochemical; Biological Assay; Black-legged Tick; Blood; Borrelia burgdorferi; Cell Culture Techniques; Cell Line; Cells; Clustered Regularly Interspaced Short Palindromic Repeats; Collaborations; Collection; Communities; Complement; Contracts; Deposition; Development; Entomology; Exposure to; Genes; Genetic; Genome; Goals; Immune; Immune response; Immune signaling; Immune system; Immunity; Immunologics; Immunology; In Vitro; Infection; Investigation; Knock-out; Knowledge; Laboratories; Mass Spectrum Analysis; Membrane; Microbiology; Minnesota; Modification; Molecular Analysis; Mutagenesis; National Institute of Allergy and Infectious Disease; Nymph; Oral; Pathogenesis; Pathway interactions; Peptides; Plasmids; Proteomics; RNA Interference; RNA delivery; Reagent; Reporter; Research; Research Personnel; Research Project Grants; Research Support; Resources; Rickettsia; Source; System; Techniques; Testing; Ticks; Training; Transcript; Translational Research; Universities; Work; base; biodefense; cost; differential expression; efficacy testing; functional genomics; gene product; genome editing; germ free condition; immune function; in vivo; insight; member; microbial; microbiota; microorganism interaction; new technology; novel; nuclease; overexpression; pathogen; professor; repository; response; symbiont; tool; transcriptomics; vector tick; web site

TICK RESOURCES CORE Abstract: The overarching goal of the Tick Resources Core within this P01 application is to provide support to all three research projects and share reagents, materials and new technologies with the scientific community. Requests from the community will be handled through the Biodefense and Emerging Infections Research Resources Repository (BEI) website (www.beiresources.org). BEI was established by the National Institute of Allergy and Infectious Diseases and has been managed under contract by the American Type Culture Collection for fifteen years. Dr. Ulrike Munderloh, a Professor at the University of Minnesota, will be the director of this technical core and is ideally suited to manage the facility. First, she has been a pioneer in the field of tick-pathogen interactions by developing and refining techniques for mutagenesis, plasmid-based complementation, and functional genomics for rickettsial agents in addition to inventing a membrane feeder system for ticks. Second, her laboratory has contributed to 22 out of 30 Rickettsiales species for sequencing in the “Rickettsiales Genomes” project, and supplied 10 of the 14 isolates deposited in BEI. Third, Dr. Munderloh has an outstanding record of distributing tick cell lines and microbial isolates to the scientific community. Aim#1 of the Tick Resources Core will provide currently available tick-vector-based tools to support the research for all three research projects within this P01 application. Aim#2 of the Tick Resources Core will develop novel cell lines suitable for analyses of immune pathways that are activated or repressed in response to pathogens, symbionts and selected microbiota. This will be achieved through RNA interference, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9), reporter tags and overexpression assays. Model organisms have a myriad of tools that facilitate the study of pathogen-arthropod interactions. They also provide critical insights to the field of entomology, microbiology and immunology. However, we present strong evidence in this P01 application that Ixodes scapularis ticks seem to be built on entirely different principles that govern microbial pathogenesis and immunity. Therefore, a strong Tick Resources Core is of utmost importance to support all three Projects within this P01 application. By providing existing I. scapularis-based tools and developing novel technologies to investigators, the Tick Resources Core will contribute to uncover novel paradigms in I. scapularis-microbial interactions. This scientific knowledge has potential implications for basic and translational science.

勾选资源核心 摘要：tick资源核心在此P01应用程序中的总体目标是为 这三个研究项目并与科学界共享试剂，材料和新技术。 社区的请求将通过生物化和新兴感染研究来处理 资源存储库（BEI）网站（www.beiresources.org）。 BEI是由美国国立研究所建立的 过敏和传染病，已由美国类型文化根据合同进行管理 收集了十五年。明尼苏达大学教授Ulrike Mundloh博士将担任董事 这是该技术核心的，非常适合管理设施。首先，她一直是该领域的先驱 通过开发和完善诱变的技术，基于质粒的技术，tick病原体相互作用 互补和人力车剂的功能基因组学，除了发明膜费 tick的系统。其次，她的实验室为30种立克种类中的22种用于测序 “立克西亚斯基因组”项目，并提供了沉积在BEI中的14个分离株中的10个。第三，Munderloh博士 在将tick细胞系和微生物分离株分发给科学界的出色记录。目标＃1 Tick Resources Core将提供当前可用的基于tick矢量的工具，以支持研究 该P01应用程序中的所有三个研究项目。 tick资源核心的目标＃2将发展出新颖的细胞 适用于分析因病原体而激活或反映的免疫病的分析的线 共生体和选定的微生物群。这将通过RNA干扰实现，定期聚类 间隔短的短文重复序列（CRISPR/CAS9），记者标签和过表达测定。模型 有机体具有无数的工具，可促进病原体 - 王毒相互作用的研究。他们也提供 对昆虫学，微生物学和免疫学领域的关键见解。但是，我们提供有力的证据 在这个P01应用程序中，ixodes capularis tick似乎建立在完全不同的原理上 微生物发病机理和免疫力。因此，强大的tick资源核心对于 支持此P01应用程序中的所有三个项目。通过提供现有的I.肩cap骨工具和 开发针对调查人员的新技术，Tick Resources Core将有助于发现新颖 I.肩cap骨相互作用的范例。这种科学知识对基本有潜在的影响 和翻译科学。