Citizen DNA Barcode Network: A Community-based Infrastructure for Monitoring Biodiversity and Disease Vectors

公民 DNA 条形码网络：用于监测生物多样性和疾病媒介的社区基础设施

基本信息

批准号：
9975528
负责人：
David Andrew MICKLOS
金额：
$ 27.07万
依托单位：
COLD SPRING HARBOR LABORATORY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-06-01 至 2025-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9975528
关键词：
Ants Arbovirus Infections Attitude Bar Codes Biochemical Biodiversity Bioinformatics Collaborations Communities County Culicidae DNA DNA Databases Data Data Analyses Databases Development Disease Vectors Education Education and Outreach Educational Materials Equipment Equipment and supply inventories Evaluation Faculty Genbank Global Warming Goals Habitats Health High School Student Infrastructure Insecta Laboratories Learning Long Island Maps Mentorship Methods Modeling Monitor Museums Nature New York Order Coleoptera Organism Participant Phase Program Evaluation Publishing Research Resources Sampling Science Scientist Site Societies Structure Surveys System Taxonomy Teacher Professional Development Techniques Training Work base citizen science climate change college design environmental change experience high school human disease improved interest member novel outreach outreach program preservation programs science education science museum success symposium vector web interface

项目摘要

In collaboration with the New York Hall of Science (NYSCI), the DNA Learning Center (DNALC) of Cold Spring Harbor Laboratory (CSHL) proposes to develop a Citizen DNA Barcode Network. The project will organize national campaigns to map the ranges of species within three groups of insect: ants, mosquitoes, and beetles. These groups include bioindicators of environmental change, vectors of human disease, and economically important species whose ranges are being altered by global climate change. Citizen scientists will use the technique of DNA barcoding, which allows non-experts to identify almost any insect species, even from larval forms or damaged samples. The project is based on an integrated biochemical and bioinformatics workflow we have developed to support DNA barcoding in formal education settings. Our current SEPA project, Barcode Long Island, has undertaken DNA barcoding campaigns in the formal education system—with high school students documenting local biodiversity and the northward range expansion of several insects—novel evidence of global warming. During the development phase, we will work intensively with NYSCI to refine resources and dissemination methods to support citizen science in informal settings. In parallel, we will pilot methods to engage visitors to nature preserves and members of conservation groups, including The Nature Conservancy and Audubon Society. During the replication phase, we will onboard three additional science centers and six additional community conservation groups. Partners will be provided with training, educational materials, mobile labs, and supplies needed to generate data for insect campaigns and biodiversity surveys (bioblitzes) of locally important habitats. Mentorship by high school and college faculty with experience in DNA barcoding and extended collaborative support—including troubleshooting and data analysis—will help insure success at each site. Data collected by citizen scientists will contribute to the scientific record in two ways: 1) Barcode sequences will be published in GenBank, the authoritative DNA database, and to species-specific databases. 2) Occurrence data will be contributed to the Global Biodiversity Information Facility (GBIF) to expand existing range maps. A multi-faceted evaluation program will develop novel survey instruments to assess the impact of participation in citizen barcoding projects on attitudes toward biodiversity on global climate change.

DNA 学习中心与纽约科学馆 (NYSCI) 合作冷泉港实验室 (CSHL) 的 DNALC 提议开发公民 DNA 该项目将组织全国范围的活动来绘制条形码网络。昆虫分为三类：蚂蚁、蚊子和甲虫。包括环境变化的生物指标、人类疾病的媒介，以及经济上重要的物种，其范围正在因全球气候变化而改变。公民科学家将使用 DNA 条形码技术，该技术允许非专家识别几乎所有昆虫物种，甚至可以从幼虫形式或受损样本中识别。该项目基于我们拥有的集成生物化学和生物信息学工作流程旨在支持正规教育环境中的 DNA 条形码。长岛条形码项目已在正式的环境中开展 DNA 条形码活动教育系统——高中生记录当地的生物多样性和几种昆虫的活动范围向北扩张——全球变暖的新证据。在开发阶段，我们将与NYSCI紧密合作，提炼资源同时，我们还制定了在非正式环境中支持公民科学的传播方法。将试点吸引游客参观自然保护区和保护成员的方法团体，包括大自然保护协会和奥杜邦协会。阶段，我们将加入三个额外的科学中心和六个额外的社区将为合作伙伴提供培训、教育材料、移动设备。为昆虫活动和生物多样性调查生成数据所需的实验室和用品高中和大学教师对当地重要栖息地的指导（生物闪电战）。 DNA 条形码和扩展协作支持方面的经验，包括故障排除和数据分析——将有助于确保每个站点的成功。公民科学家收集的数据将以两种方式为科学记录做出贡献：1) 条码序列将发布在权威DNA数据库GenBank中， 2) 发生数据将贡献给全球数据库。生物多样性信息设施（GBIF）扩展现有的范围图。评估计划将开发新颖的调查工具来评估参与关于全球生物多样性态度的公民条形码项目气候变化。