Harnessing natural stem cell-based strategies for mammalian dental renewal

利用基于天然干细胞的策略进行哺乳动物牙齿更新

基本信息

批准号：
9924957
负责人：
Ophir D Klein
金额：
$ 10.08万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-09-22 至 2021-06-30
项目状态：
已结题

项目摘要

PROJECT SUMMARY/ABSTRACT By discovering the strategies that animals normally use to renew and regenerate their tissues, we can lay a foundation for regenerative approaches in humans. One of the principal long-term goals of my laboratory is to understand mammalian renewal and regeneration by integrating developmental and evolutionary perspectives. To do this, we are using the dentition as a model and are taking advantage of a remarkable property that is found in diverse mammalian species, which is the ability to grow teeth continuously based on the presence of adult stem cells. Despite initial progress, many gaps and challenges in our knowledge remain. We are still in the early stages of understanding how dental stem cells in the incisor epithelium behave, and we know very little about the mesenchymal stem cells. We also have a great deal to learn about how dental stem cells evolved and how they arise in the embryo during development. The overall scope of the research program proposed here will be to contend with these fundamental questions: How do adult stem cells enable the organ to renew? How do the stem cells in teeth respond to environmental influences such as hibernation and force? How did stem cells emerge during evolution of the mammalian dentition? How do progenitor cells in the embryo provide the starting materials for tooth development? Can we target tooth progenitors to cure disease? The impact of our work will be to provide a solid basic science foundation for future regenerative approaches by obtaining deep insight into a system of natural mammalian dental renewal.

项目摘要/摘要通过发现通常使用动物更新和再生其组织的策略，我们可以放置一个人类再生方法的基础。我实验室的主要长期目标之一是通过整合发展和进化来了解哺乳动物的更新和再生观点。为此，我们将牙齿用作模型，并利用了一个非凡的在多种哺乳动物物种中发现的特性，这是基于成年干细胞的存在。尽管有最初的进步，但我们所知的许多差距和挑战保持。我们仍处于了解门牙上皮中牙齿干细胞如何的早期阶段行为，我们对间充质干细胞了解甚少。我们也有很多学习在发育过程中，牙齿干细胞的演变以及它们在胚胎中的产生方式。总体范围这里提出的研究计划将解决以下基本问题：成人如何细胞使器官更新？牙齿中的干细胞如何应对环境影响，例如冬眠和力量？干细胞在哺乳动物牙列进化过程中如何出现？怎么办胚胎中的祖细胞提供牙齿发育的起始材料？我们可以瞄准牙齿吗治愈疾病的祖细胞？我们工作的影响将是提供坚实的基础科学基金会对于将来的再生方法，通过深入了解天然哺乳动物系统牙科更新。