Rice Bran Microbial Metabolism for Colon Chemoprevention

米糠微生物代谢用于结肠化学预防

• 批准号: 9916241
• 负责人: Komal Raina
• 金额: $ 14.63万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2021-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9916241
• 关键词: Attention; Award; Azoxymethane; Bacteria; Biochemical Pathway; Botanicals; Chemoprevention; Chronic Disease; Colon; Colon Carcinoma; Communities; Computer Analysis; Consumption; Data; Data Set; Development; Diet; Dietary Intervention; Disputes; Environment; Enzymes; Feces; Female; Formulation; Future; Gene Expression; Genes; Genetic Transcription; Health; Health Benefit; Health Promotion; Human; Industry; Inflammation; Intestines; Investigation; Knowledge; Metabolic; Metabolic Pathway; Metabolism; Microbe; Mus; Oral; Organism; Parents; Probiotics; Proteins; Proteomics; RNA; Research; Research Support; Rice; Risk; Safety; Sodium Dextran Sulfate; Soil; Supplementation; Taxonomy; Testing; base; colon cancer prevention; colon carcinogenesis; comparative; dietary supplements; fecal microbiome; gut colonization; improved; innovation; male; metaproteomics; metatranscriptome; metatranscriptomics; microbial; microbial community; microbiome; microbiota; microorganism; novel; oral supplementation; plant growth/development; protein metabolite; trait; transcriptomics; wasting; water treatment

Project Summary There is a need to advance oral dietary supplement research to include development of microbial communities that support colon health and metabolism by reducing colon inflammation. This approach should not be confused with traditional approaches that have combined microbes into a supplement, and notably that the strains did not originate as a microbial community. Preliminary data support that oral delivery of a set of human stool microbes to mice conferred protection against colon inflammation and prevention of colon cancer. The microbial communities that were delivered orally to mice were derived from people that consumed a rice bran rich diet for one month. The parent R01 award is elucidating mechanisms by which a rice bran diet modified the human stool microbiome in a manner that provided protection from colon carcinogenesis. This supplement award proposes to functionally characterize the microbial community that is being delivered as an oral inoculum and will advance the field of microbial based dietary supplements using computational, integrated functional analysis. In this one- year supplement award period, we propose to perform a comprehensive meta-transcriptomic and metaproteomic investigation using an innovative workflow for functional characterization of colon health promoting microbial consortia. This is considered essential prior to development and use as microbial consortia-based dietary supplements. We hypothesize that human stool microbial communities that reduced colon inflammation can be safety developed when analyzed for functional health benefits as a microbial consortia. We will use stool microbial communities that were already collected from 5 females and 5 males before and after completing dietary intervention. The oral delivery of stool microbial consortia to mice were selected for use in this project based on functional efficacy. Employing metatranscriptomics to profile transcriptionally active genes in the microbial inoculum and therefore metabolically active microbes will be correlated with functional effects on the colon. We will also complete metaproteomics to understand what proteins/enzyme functions are actively produced and by which microbes; a traceability that extends beyond single microbial contribution to ascertain microbial community function. The application and integration of multiple meta-omics is essential to provide a complete mechanistic understanding of the candidate oral "microbial consortium". This project will also develop a computational workflow for functional metabolic network mapping of each microbial consortia that will support future testing as an oral dietary supplement.

项目摘要 有必要推进口头饮食补充研究以包括微生物群落的发展 通过减少结肠炎症来支持结肠健康和代谢。这种方法不应该困惑 通过将微生物合并为补充的传统方法，尤其是菌株没有 起源于微生物社区。初步数据支持口服一组人粪便微生物 小鼠赋予了防止结肠炎症和预防结肠癌的保护。微生物 口服给小鼠的社区源自食用米麸富饮食的人 一个月。家长R01奖是阐明米麸饮食修改人粪便的机制 微生物组的方式可保护结肠癌发生。该补充奖提议 在功能上表征正在作为口服接种物传递的微生物社区，并将推进 使用计算，集成功能分析的基于微生物的饮食补充剂领域。在这个 年度补充奖励期，我们建议执行全面的荟萃分数和元蛋白质组学 使用创新的工作流进行调查，以促进微生物结肠健康的功能表征 财团。这在开发之前被认为是必不可少的，并用作基于微生物财团的饮食 补品。我们假设人类粪便微生物群落减少结肠炎症可能是 当分析作为微生物联盟的功能健康益处时，安全开发了。我们将使用凳子 完成前后已经从5名女性和5名男性收集的微生物群落 饮食干预。选择了向小鼠的粪便微生物财团的口服输送在此项目中使用 基于功能功效。在谱图中采用元文字组合图中的转录活性基因 微生物接种物，因此代谢活性微生物将与对该功能的影响相关 冒号。我们还将完成元蛋白质组学，以了解哪些蛋白质/酶功能是积极的 产生并通过哪些微生物；可追溯性超出了单个微生物的贡献以确定 微生物社区功能。多个元论的应用和集成对于提供一个 对候选人口服“微生物联盟”的完全机械理解。这个项目也将发展 每个微生物联盟的功能代谢网络映射的计算工作流，将支持 将来作为口服饮食补充剂进行测试。