Rice Bran Microbial Metabolism for Colon Chemoprevention

米糠微生物代谢用于结肠化学预防

• 批准号: 9916241
• 负责人: Komal Raina
• 金额: $ 14.63万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2021-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9916241
• 关键词: Attention; Award; Azoxymethane; Bacteria; Biochemical Pathway; Botanicals; Chemoprevention; Chronic Disease; Colon; Colon Carcinoma; Communities; Computer Analysis; Consumption; Data; Data Set; Development; Diet; Dietary Intervention; Disputes; Environment; Enzymes; Feces; Female; Formulation; Future; Gene Expression; Genes; Genetic Transcription; Health; Health Benefit; Health Promotion; Human; Industry; Inflammation; Intestines; Investigation; Knowledge; Metabolic; Metabolic Pathway; Metabolism; Microbe; Mus; Oral; Organism; Parents; Probiotics; Proteins; Proteomics; RNA; Research; Research Support; Rice; Risk; Safety; Sodium Dextran Sulfate; Soil; Supplementation; Taxonomy; Testing; base; colon cancer prevention; colon carcinogenesis; comparative; dietary supplements; fecal microbiome; gut colonization; improved; innovation; male; metaproteomics; metatranscriptome; metatranscriptomics; microbial; microbial community; microbiome; microbiota; microorganism; novel; oral supplementation; plant growth/development; protein metabolite; trait; transcriptomics; wasting; water treatment

Project Summary There is a need to advance oral dietary supplement research to include development of microbial communities that support colon health and metabolism by reducing colon inflammation. This approach should not be confused with traditional approaches that have combined microbes into a supplement, and notably that the strains did not originate as a microbial community. Preliminary data support that oral delivery of a set of human stool microbes to mice conferred protection against colon inflammation and prevention of colon cancer. The microbial communities that were delivered orally to mice were derived from people that consumed a rice bran rich diet for one month. The parent R01 award is elucidating mechanisms by which a rice bran diet modified the human stool microbiome in a manner that provided protection from colon carcinogenesis. This supplement award proposes to functionally characterize the microbial community that is being delivered as an oral inoculum and will advance the field of microbial based dietary supplements using computational, integrated functional analysis. In this one- year supplement award period, we propose to perform a comprehensive meta-transcriptomic and metaproteomic investigation using an innovative workflow for functional characterization of colon health promoting microbial consortia. This is considered essential prior to development and use as microbial consortia-based dietary supplements. We hypothesize that human stool microbial communities that reduced colon inflammation can be safety developed when analyzed for functional health benefits as a microbial consortia. We will use stool microbial communities that were already collected from 5 females and 5 males before and after completing dietary intervention. The oral delivery of stool microbial consortia to mice were selected for use in this project based on functional efficacy. Employing metatranscriptomics to profile transcriptionally active genes in the microbial inoculum and therefore metabolically active microbes will be correlated with functional effects on the colon. We will also complete metaproteomics to understand what proteins/enzyme functions are actively produced and by which microbes; a traceability that extends beyond single microbial contribution to ascertain microbial community function. The application and integration of multiple meta-omics is essential to provide a complete mechanistic understanding of the candidate oral "microbial consortium". This project will also develop a computational workflow for functional metabolic network mapping of each microbial consortia that will support future testing as an oral dietary supplement.

项目概要 需要推进口服膳食补充剂研究，包括微生物群落的发展 通过减少结肠炎症来支持结肠健康和新陈代谢。这种方法不应该被混淆 传统方法将微生物组合成补充剂，值得注意的是，这些菌株并没有 起源于微生物群落。初步数据支持口服输送一组人类粪便微生物 给小鼠提供了针对结肠炎症和预防结肠癌的保护作用。微生物 口服给老鼠的群落来自那些长期食用富含米糠的饮食的人 一个月。父级 R01 奖旨在阐明米糠饮食改变人类粪便的机制 微生物组以提供保护免受结肠癌发生的方式。该补充奖建议 对作为口服接种物提供的微生物群落进行功能表征，并将推进 使用计算、集成功能分析的基于微生物的膳食补充剂领域。在这一篇中—— 年补充奖励期间，我们建议进行全面的宏转录组学和宏蛋白质组学 使用创新工作流程进行结肠健康促进微生物功能表征的研究 财团。在开发和使用基于微生物群落的饮食之前，这被认为是必不可少的 补充品。我们假设减少结肠炎症的人类粪便微生物群落可能是 通过对微生物群落的功能性健康益处进行分析，得出了安全性。我们会用凳子 完成之前和之后从 5 名女性和 5 名男性身上收集的微生物群落 饮食干预。本项目选择使用向小鼠口服粪便微生物群落的方法 基于功能功效。利用宏转录组学来分析转录活性基因 微生物接种物以及因此具有代谢活性的微生物将与对机体的功能影响相关。 冒号。我们还将完成宏蛋白质组学，以了解哪些蛋白质/酶功能是活跃的 由哪些微生物产生；可追溯性超越了单一微生物的贡献来确定 微生物群落功能。多种元组学的应用和整合对于提供 对候选人口腔“微生物联盟”的完整机制了解。该项目还将开发 每个微生物群落的功能代谢网络映射的计算工作流程将支持 未来作为口服膳食补充剂进行测试。