ICES-based Pulsed Field Electromagnetic Field Therapy for Autoimmunity

基于 ICES 的自身免疫脉冲场电磁场治疗

基本信息

批准号：
9903662
负责人：
Roland M Tisch
金额：
$ 23.33万
依托单位：
UNIV OF NORTH CAROLINA CHAPEL HILL
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-01-06 至 2021-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9903662
关键词：
Antigens Autoimmune Diseases Autoimmunity Automobile Driving Biological Biophysical Process Cell Maturation Cell physiology Cells Clinic Complex Coupled Data Dendritic Cells Development Effectiveness Effector Cell Electric Stimulation Electromagnetic Fields Electromagnetics Electrostatics Eragrostis Event FOXP3 gene Foundations Future Goals Heterogeneity Human Human Activities Immune Immunotherapy Inbred NOD Mice Inflammation Insulin-Dependent Diabetes Mellitus Mediating Mind Modeling Molecular Nature Orthopedics Pathology Phenotype Physiologic pulse Prevention Property Proteins Pulse Rates Regulatory T-Lymphocyte Reporting Rest Safety Self Tolerance Shapes Signal Transduction Site T-Lymphocyte Testing Therapeutic Tissues Work acquired immunity autoreactivity base cell type clinical application combinatorial disease heterogeneity disorder prevention effector T cell humanized mouse immunopathology immunoregulation in vivo innovation insight islet nano novel pre-clinical prevent tool

项目摘要

SUMMARY/ABSTRACT Human autoimmune diseases such as type 1 diabetes (T1D) are characterized by heterogeneous and complex events driving immunopathology. To deal with this heterogeneity for disease prevention and treatment, we need a variety of immunotherapies with distinct mechanisms of action. With this in mind, the goal of our R21 proposal is to establish proof-of-concept that pulsed electromagnetic field (PEMF) therapy is an effective approach to selectively suppress ongoing autoimmunity, and reestablish self-tolerance. Micro/nano-currents induced in vivo by PEMF under the appropriate conditions can alter cellular properties. Different types of PEMF have been successfully and safely applied in the clinic; however, use of PEMF for autoimmunity has not been tested. For this study, we are using a unique type of PEMF referred to as inductively coupled electrical stimulation (ICES). Properties of ICES result in electromagnetic pulses believed to closely mimic native electrical signaling events that enhance both biological effects and safety. Three Aims are outlined, employing NOD mice a spontaneous model of T1D, to establish the applicability of ICES-based PEMF to modulate autoimmunity. In Aim 1, studies will define the tolerogenic effects of ICES on  cell-specific T cell reactivity. Aim 2 will focus on determining ICES effects on dendritic cell maturation and function. In Aim 3 ICES-induced immunoregulation will be explored, and effects on acquired immunity investigated. This work is expected to establish a foundation for future studies: 1) to further define the mechanisms by which ICES specifically, and PEMF in general regulate immune effectors, and 2) to test the clinical application of ICES to prevent and/or treat T1D, as well as other autoimmune diseases and immune-driven pathologies.

摘要/摘要人类自身免疫性疾病，如 1 型糖尿病 (T1D)，具有异质性和复杂性的特点为了应对疾病预防和治疗的异质性，我们需要具有不同作用机制的多种免疫疗法，这就是我们 R21 的目标。该提案旨在验证脉冲电磁场 (PEMF) 疗法是一种有效的治疗方法选择性抑制正在进行的自身免疫并重建自我耐受性的方法。在适当条件下在体内诱导的 PEMF 可以改变细胞特性。已成功并安全地应用于临床；然而，PEMF 用于自身免疫尚未得到应用。已测试。在本研究中，我们使用一种独特类型的 PEMF，称为感应耦合电刺激 (ICES) 的特性导致电磁脉冲被认为非常模仿天然的电信号。概述了使用 NOD 小鼠增强生物效应和安全性的三个目标。 T1D 自发模型，建立基于 ICES 的 PEMF 调节自身免疫的适用性。目标 1，研究将确定 ICES 对  细胞特异性 T 细胞反应性的耐受性影响。目标 2 将重点关注。确定 ICES 对树突状细胞成熟和功能的影响目标 3 ICES 诱导的免疫调节。将进行探索，并研究对获得性免疫的影响，预计这项工作将奠定基础。对于未来的研究：1）进一步定义ICES具体和PEMF一般的机制调节免疫效应器，2) 测试 ICES 预防和/或治疗 T1D 的临床应用，以及其他自身免疫性疾病和免疫驱动的病理。