RNA scanning by regulatory endonucleases - Resubmission - 1

通过调节核酸内切酶进行 RNA 扫描 - 重新提交 - 1

基本信息

批准号：
9901585
负责人：
JOEL G BELASCO
金额：
$ 40.04万
依托单位：
NEW YORK UNIVERSITY SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-04-06 至 2022-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9901585
关键词：
3-Dimensional Address Affect Amino Acids Bacteria Bacterial Genome Base Pairing Binding Binding Sites Biochemical Genetics Biological Biology Catalytic Domain Cells Characteristics Complementary RNA Distant Ensure Enzymes Escherichia coli Excision Gene Expression Gene Expression Regulation Genes Genetic Transcription Goals Homologous Gene Human Knowledge Medical Messenger RNA Methods Molecular Nucleotides Occupations Organism Orthologous Gene Pathogenesis Pathogenicity Phosphoric Monoester Hydrolases Play Process Production Property Proteins RNA RNA Binding RNA Degradation RNA Processing RNA annealing Research Research Project Grants Ribonucleases Ribosomes Role Scanning Side Site Stress Structure Transcript Untranslated RNA Ursidae Family endonuclease inorganic phosphate insight mRNA Decay mRNA Transcript Degradation novel paralogous gene phosphodiester preference public health relevance ribonuclease E single-molecule FRET

项目摘要

PROJECT SUMMARY DESCRIPTION: The long-term goal of this research project is to understand the basic principles that govern mRNA degradation, a process that has long been known to play a key role in controlling gene expression in all organisms. In Escherichia coli and related bacterial species, the ribonuclease with the greatest influence on mRNA lifetimes is RNase E, an endonuclease with a strong preference for decay intermediates that bear a single phosphate at the 5′ terminus. Recent evidence indicates that RNase E locates its cleavage sites in monophosphorylated RNA by scanning linearly downstream from the 5′ end along RNA segments that are single-stranded. The immediate objectives of this research project are to elucidate the mechanism of RNase E scanning, the enzyme characteristics that make it possible, the features of RNA that facilitate or impede it, and the impact of this process on gene expression. Achieving these objectives will require the use of standard molecular biological, biochemical, and genetic methods as well as single-molecule FRET. The knowledge gained from these studies will provide fundamental insights into a novel aspect of gene regulation that is likely to be important for bacterial pathogenesis.

项目摘要描述：该研究项目的长期目标是了解基本控制mRNA降解的原则，这一过程长期以来一直在发挥钥匙在控制所有生物中基因表达中的作用。在大肠杆菌和相关细菌中物种，对mRNA寿命影响最大的核糖核酸酶是RNase E，一个核酸内切酶对衰减中间体具有强烈的偏爱 5'末端。最近的证据表明RNase E将其切割位点定位在单磷酸化的RNA通过从5'端沿RNA线性扫描下游扫描RNA 单链的段。该研究项目的直接目标是阐明RNase E扫描的机制，这是使其特征的酶特性可能，促进或阻碍它的RNA的特征以及该过程对基因的影响表达。实现这些对象将需要使用标准分子生物学，生化方法和遗传学方法以及单分子特品格。知识获得了从这些研究中将提供对基因调节的新方面的基本见解，对于细菌发病机理可能很重要。