Senescence-Chips for Radiation Biodosimetry

用于辐射生物剂量测定的衰老芯片

基本信息

批准号：
9901427
负责人：
DAOJING WANG
金额：
$ 99.74万
依托单位：
NEWOMICS, INC
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-08-05 至 2022-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9901427
关键词：
3-Dimensional Acute Bioinformatics Biological Biological Assay Biological Markers Blood Blood Plasma Volume Blood specimen Bone Marrow Businesses C57BL/6 Mouse Cell Size Cells Cellular Assay Clinical Detection Development Diagnosis Dose Emergency Situation Excision Hematopoietic stem cells Human Inbred BALB C Mice Ionizing radiation Lipids Macaca mulatta Measurable Measurement Medical Mesenchymal Stem Cells Microfluidic Microchips Molecular Analysis Monkeys Mouse Strains Mus National Institute of Allergy and Infectious Disease Nuclear Nuclear Accidents Nuclear Radiology Pathway interactions Phase Phenotype Proteomics Radiation Radiation Accidents Radiation Injuries Radiation exposure Reproducibility Resistance Resolution Sampling Sensitivity and Specificity Shotguns Small Business Innovation Research Grant Stains Structure Technology Testing Time Triage Whole Blood Whole-Body Irradiation biodosimetry biomarker panel commercialization cost design improved in vivo industry partner innovation medical countermeasure metabolomics minimally invasive molecular marker mouse model multiple omics novel operation peripheral blood potential biomarker predictive test programs radiation response radioresistant research and development response scale up senescence small molecule tissue injury

项目摘要

Ideal biomarkers for acute and delayed radiation injury after a radiological/nuclear terrorist incident are those that arise and are measurable prior to manifestation of tissue injuries, typically one to a few days after ionizing radiation (IR) exposure. However, there are currently no commercial platforms that could perform assays for these biomarkers with high robustness, sensitivity, and specificity for radiation biodosimetry. In response to PA- 18-525, Newomics Inc. proposes in this Phase II project to complete development of its product, termed “Senescence-Chips”, for rapid and accurate detection of senescent cells, particularly, IR-induced senescent cells, for radiation biodosimetry and medical countermeasure. The Phase II project is built upon the promising results generated from our Phase I project. In Phase I, we have developed the integrated microfluidic chips, and demonstrated their proof-of-principle applications for ultrahigh-throughput isolation and removal of senescent cells spiked in whole blood. In Phase II, we will complete development of the senescence-chips for commercialization, and develop new multiomics assays for analyzing molecular marker panels using Newomics' breakthrough multinozzle emitter array chips. Once developed, Newomics' integrated platforms and solutions will enable low-cost, reproducible, highly specific and sensitive multiplex measurements of human peripheral blood for radiation biodosimetry, thus serving as a field-deployable platform for radiological/nuclear medical countermeasures including emergency triage and medical responses.

放射性/核恐怖事件后急性和迟发性辐射损伤的理想生物标志物是在组织损伤出现之前出现并可测量，通常是在电离后一到几天然而，目前还没有可以进行辐射（IR）暴露检测的商业平台。这些生物标志物对于辐射生物剂量测定具有高稳健性、敏感性和特异性。 18-525，Newomics Inc. 提议在此第二阶段项目中完成其产品的开发，称为 “衰老芯片”，用于快速准确地检测衰老细胞，特别是红外线诱导的衰老细胞细胞，用于辐射生物剂量测定和医疗对策第二阶段项目是建立在有前途的基础上的。我们第一阶段项目产生的结果在第一阶段，我们开发了集成微流控芯片，以及他们的原理验证展示了超高通量分离和去除衰老的应用在第二阶段，我们将完成衰老芯片的开发。商业化，并开发新的多组学测定，用于使用 Newomics 分析分子标记组突破性的多喷嘴发射器阵列芯片一旦开发出来，Newomics 的集成平台和解决方案。将实现对人体外围设备的低成本、可重复、高度特异性和灵敏的多重测量用于辐射生物剂量测定的血液，从而作为放射/核医学的现场部署平台对策包括紧急分类和医疗响应。