Non-hydrolysable analogs of retinal chromophore; potential new therapeutics to prevent retinal degeneration

视网膜发色团的不可水解类似物；

基本信息

批准号：
9899990
负责人：
Beata Jastrzebska
金额：
$ 35.66万
依托单位：
CASE WESTERN RESERVE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-04-01 至 2021-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9899990
关键词：
11 cis Retinal Age related macular degeneration Aldehydes All-Trans-Retinol Alternative Therapies Amines Animal Model Animals Apoproteins Attenuated Binding Binding Sites Biochemical Biochemical Reaction Biological Assay Blindness Brain Bypass Caenorhabditis elegans Carbon Carrier Proteins Cattle Cells Chemicals Chlorides Cognitive Complex Cultured Cells Cyclic AMP Dark Adaptation Degenerative Disorder Development Diet Digestion Disease Drug Kinetics Drug or chemical Tissue Distribution Electroretinography Enzymes Event Exhibits Exposure to Eye Functional disorder GTP-Binding Proteins Genetic Health High Pressure Liquid Chromatography Histologic Human Imaging Techniques Impairment In Situ In Vitro Incubated Isomerism Knowledge Lasers Leber&apos s amaurosis Light Lighting Mass Spectrum Analysis Mediating Membrane Methods Modeling Monitor Mouse Protein Mus Natural regeneration Nature Nitrogen Ophthalmoscopy Opsin Optical Coherence Tomography Oral Administration Photons Photoreceptors Physiology Pigments Property Proteins Recovery Recycling Reporting Retina Retinal Degeneration Retinal Diseases Retinal Pigments Retinitis Pigmentosa Retinoids Retinol Binding Proteins Rhodopsin Rod Role Scanning Schiff Bases Series Serum Signal Transduction Sorsby&apos s fundus dystrophy Stargardt&apos s disease Testing Therapeutic Toxic effect Vision Visual Vitamin A Vitamin A Deficiency absorption analog base chemical synthesis chromophore combat desensitization early onset mouse model non-invasive imaging non-invasive monitor novel novel therapeutics preservation prevent protective effect protein activation public health relevance regenerative retinal rods risk minimization rod outer segment disc side effect stem two photon microscopy uptake visual cycle

项目摘要

DESCRIPTION (provided by applicant): An insufficient supply of visual chromophore due to dysfunction of key proteins involved in its regeneration has devastating effects on rod-mediated vision, and comprises a leading cause of irreversible blindness in humans. Early signs of such blinding diseases are delayed rod cell-mediated dark adaptation and difficulty with night vision. The decline in recovery of visual sensitivity is likely caused by inadequate Rho regeneration with accumulation of chromophore-free opsin that constitutively activates the signaling cascade and accelerates retinal degeneration. Although such free opsin activity can be reduced by exogenous retinal chromophore, this fails to prevent the buildup of toxic retinoid photo-products when their clearance is defective. Thus, an alternative therapeutic approach is urgently needed for combating vision loss under such conditions. Here we propose to investigate the effects of new visual pigments, retinyl-opsins regenerated with novel chromophore analogs, retinyl chlorides on retina physiology in context of potential therapeutic strategy to protect retinal healh in retinal degenerative diseases associated with compromised Rho regeneration. First, we will study the biochemical and functional properties of different retinyl chloride isomers in vitro (Aim 1), and then test the effects of selected retinyl chlorides in both Abca4-/-Rdh8-/- mice, a model of early onset human Stargardt disease and in Lrat-/- mice, a model of Leber congenital amaurosis (LCA) (Aim 2). Finally, we will assess the capability of the RBP4 carrier to increase the ocular delivery of these compounds, and thereby alleviate progressive retinal degeneration in these mouse models (Aim 3).

描述（由适用提供）：由于其再生所涉及的关键蛋白功能障碍引起的视觉发色团供应不足，对杆介导的视力产生了毁灭性的影响，并且是人类不可逆失明的主要原因。这种盲疾病的早期迹象是延迟的杆细胞介导的黑暗适应性，并且在夜视范围内很难。视觉敏感性恢复的下降可能是由于Rho再生不足而引起的，而无色蛋白的积累则可以组成式地激活信号传导级联并加速视网膜变性。尽管外源残留的发色团可以降低这种自由的Opsin活性，但在清除有缺陷时，这无法防止有毒的类维生素性照片产生产生。这是在这种情况下迫切需要一种替代治疗方法来打击视力丧失。在这里，我们建议研究新的视觉色素，新型视网膜粘蛋白，与新型的发色团类似物，视乙酰氯化物再生，在潜在的治疗策略中保护与损害Rho Rho再生有关的视网膜退行性疾病中的视网膜疗法的潜在治疗策略。首先，我们将研究体外不同视乙酰氯异构体的生化和功能特性（AIM 1），然后测试选定的视乙酰氯化物在ABCA4 - / - RDH8 - / - 小鼠中的作用，早期发作的人类Stargardt疾病和LRAT - / - 小鼠的模型，即Leber先天性阿莫斯菌（LCA）的模型（AIM 2）。最后，我们将评估RBP4载体增加这些化合物的眼部输送的能力，从而减轻这些小鼠模型的渐进性视网膜变性（AIM 3）。