ShEEP Equipment Request for GeoMx™ Digital Spatial Profiler System

SheEEP GeoMx™ 数字空间剖面仪系统的设备请求

• 批准号: 9906020
• 负责人: Wenhan Chang
• 金额: --
• 依托单位: VETERANS AFFAIRS MED CTR SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9906020
• 关键词: Animal Model; Animals; Antibodies; Area; Award; Basic Science; Biochemical; Biological; Biological Process; Biology; Biomechanics; Biomedical Research; Biopsy; Bone Diseases; Brain Diseases; California; Cancer Biology; Cells; Code; Collaborations; Color; Communities; Core Facility; Dermatology; Detection; Development; Disease; Disease Progression; Endocrinology; Equipment; Expression Profiling; Formalin; Funding; Gene Expression; Gene Proteins; Genetic Transcription; Goals; Human; Immunohistochemistry; Immunology; In Situ Hybridization; Medical center; Molecular; Neurobiology; Nucleotides; Operative Surgical Procedures; Orthopedics; Outcome; Paraffin Embedding; Patients; Population; Proteins; RNA; Reagent; Research; Research Personnel; Resolution; Role; San Francisco; Services; Sheep; Specimen; System; Techniques; Technology; Tissue Embedding; Tissues; Transcript; Translational Research; United States National Institutes of Health; Universities; Western Blotting; animal tissue; clinical investigation; cost; digital; disease diagnosis; instrument; instrumentation; interest; nano-string; programs; protein expression; protein profiling; skeletal; skin disorder; tool

This application is to acquire a GeoMx™ Digital Spatial Profiler System (NanoString Technology) on behalf of the San Francisco VA Medical Center (SFVAMC) Skeletal Biology and Biomechanics Core Facility, which was originally established in 2003 by funding of BLR&D Research Enhancement Award Program and later a BLR&D Program Project. This Core had supported more than 70 VA, NIH, and DoD-funded projects in endocrinology, orthopedics surgery, neurobiology, immunology, and cardiothoracic surgery, and enabled numerous new collaborations in basic and translational research within the greater UCSF research community. The proposed acquisition of this state-of-the-art GeoMx™ Digital Spatial Profiler System, which is optimized to facilitate analyses of biological specimens and outcomes, is aimed to further expand our Core capacity for translational and clinical investigation. This tool will allow investigators accessing this Core to investigate the cellular, molecular, and biochemical features of cells and tissues from animal studies and patient biopsies in a high-throughput and highly quantitative manner. Given the critical role of the molecular changes (RNA and protein expression) in biological processes throughout development and disease, this equipment would allow our investigators to facilitate existing studies and pursue unexplored area of research with an efficiency and accuracy that could not be achieved by the current instrumentation in the Core. This new instrumentation is anticipated in the short term to greatly expand the research scope of 9 active VA- funded projects and more than 9 NIH and DoD projects awarded to the participating investigators.

此申请是代表获取GeoMx™数字空间剖面仪系统（NanoString技术） 旧金山退伍军人医疗中心 (SFVAMC) 骨骼生物学和生物力学核心设施， 最初成立于 2003 年，由 BLR&D 研究增强奖励计划资助，后来又成立 BLR&D 计划项目支持了 70 多个 VA、NIH 和 DoD 资助的项目。 内分泌学、骨科手术、神经生物学、免疫学和心胸外科，并启用 加州大学旧金山分校研究范围内基础和转化研究方面的众多新合作 社区提议收购最先进的 GeoMx™ 数字空间剖面仪系统。 经过优化以促进生物样本和结果的分析，旨在进一步扩展我们的核心 该工具将允许研究人员访问该核心以进行转化和临床研究。 研究动物研究中细胞和组织的细胞、分子和生化特征 鉴于分子的关键作用，以高通量和高定量的方式对患者进行活检。 整个发育和疾病过程中生物过程的变化（RNA和蛋白质表达），这 设备将使我们的研究人员能够促进现有研究并探索未探索的研究领域 其效率和准确性是 Core This 中当前仪器无法达到的。 预计新仪器在短期内将大大扩展 9 种主动 VA- 的研究范围 资助的项目以及超过 9 个 NIH 和 DoD 项目授予参与研究人员。