Surface antimicrobial coating to prevent bacterial biofilms

表面抗菌涂层可防止细菌生物膜

• 批准号: 9388956
• 负责人: GUANGSHUN WANG
• 金额: $ 7.53万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-12-01 至 2018-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9388956
• 关键词: Abdomen; Antibiotic Resistance; Antibiotic Therapy; Antibiotics; Antimicrobial Effect; Bacteria; Bacterial Infections; Blood Vessel Prosthesis; Cardiac; Catheters; Cells; Charge; Chemicals; Data; Databases; Development; Devices; Environment; Evolution; Family; Glycine; Gram-Negative Bacteria; Hospitals; Human; Immobilization; Infection; Laboratories; Lead; Life; Limb structure; Link; Location; Medical; Medical Device; Membrane; Methods; Microbial Biofilms; Modeling; Nosocomial Infections; Outcome; Pain; Patients; Peptides; Polyethylene Terephthalates; Polymers; Positioning Attribute; Postdoctoral Fellow; Process; Property; Reporting; Research; Research Personnel; Research Project Grants; Resistance; Resort; Staphylococcus aureus; Superbug; Surface; Technology; Testing; Time; Toxic effect; Work; antimicrobial; antimicrobial peptide; bacterial community; bacterial resistance; base; career; cost; design; flexibility; improved; innovation; interest; medical implant; mimetics; natural antimicrobial; novel; pathogen; peptide structure; prevent; product development; protein aminoacid sequence; success; training opportunity

Project Summary Implant medical devices are widely utilized as an essential support to patients. It is projected that many people will use at least one such device during their life time. However, there are medical issues with these devices such as bacterial infection. In particular, the formation of bacterial biofilms in implant medical devices further complicates the problem. Biofilms are bacterial communities that work together to escape the killing by the host or antibiotics. Currently, there is no treatment for such biofilms on medical devices and the last solution is to replace the infected ones. Such a replacement is costly and painful, however. Therefore, the objective of this proposal is to develop novel antimicrobial peptides and immobilization technologies that prevent bacterial biofilm formation on polymer materials of medical significance. Unlike traditional antibiotics, antimicrobial peptides remain potent after millions of years' evolution. We hypothesize that the immobilization of short antimicrobial peptides of varying activities can prevent biofilm formation on the polyethylene terephthalate (PET) surface. To test our hypothesis, we have designed the following specific aims: (1) To optimize the short antimicrobial peptide by sequence permutation; and (2) To immobilize the optimized peptide to the polyethylene terephthalate surface. We are well prepared to pursue this project. We have obtained preliminary results that support the feasibility of this research in the PI's laboratory. The anticipated outcomes of this R03 project are two fold. First, this project will lead to a family of short peptides with varying activity against superbugs. Second, the development of novel peptide immobilization technology will bring forth new possibilities to patients who are in need of new implant medical devices with improved properties. Finally, the project also provides an excellent training opportunity to the PI's postdoctoral fellow who will pursue an independent career in this direction.

项目摘要 植入医疗设备被广泛用作对患者的基本支持。预计 许多人一生中将至少使用一种这样的设备。但是，有医疗问题 使用这些设备，例如细菌感染。特别是，植入物中细菌生物膜的形成 医疗设备进一步使问题复杂化。生物膜是共同努力的细菌群落 避开宿主或抗生素的杀戮。目前，这种生物膜在医学上没有治疗 设备和最后一个解决方案是替换受感染的解决方案。这样的替代品是昂贵和痛苦的， 然而。因此，该提议的目的是开发新型抗菌肽和 固定技术可以防止在医学的聚合物材料上形成细菌生物膜 意义。与传统的抗生素不同，抗菌肽在数百万年后保持有效 进化。我们假设固定在不同活动的短抗菌肽可以 防止在聚乙烯二苯二甲酸酯（PET）表面形成生物膜。为了检验我们的假设，我们 已经设计了以下特定目的：（1）按顺序优化短抗菌肽 排列; （2）将优化的肽固定在聚对苯二甲酸酯表面上。我们 已经准备好追求这个项目。我们获得了支持可行性的初步结果 在PI实验室的这项研究中。该R03项目的预期结果是两个倍。第一的， 该项目将导致一个短肽家族对超级细菌的活动有所不同。第二， 新型肽固定技术的开发将为患者带来新的可能性 需要具有改进特性的新植入医疗设备。最后，该项目还提供了 Pi的博士后研究员的绝佳培训机会，他们将在此领域从事独立职业 方向。