Multifunctional Ionic Liquids Coatings for Dental Implant Surfaces

用于牙种植体表面的多功能离子液体涂层

• 批准号: 9768425
• 负责人: Danieli Rodrigues
• 金额: $ 36.34万
• 依托单位: UNIVERSITY OF TEXAS DALLAS
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-14 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9768425
• 关键词: Adhesions; Animal Model; Animals; Anions; Bacteria; Bacterial Adhesion; Bacterial Infections; Bone Growth; Bone Tissue; Cell surface; Cells; Clinical; Coculture Techniques; Corrosion; Corrosives; Dental Implants; Diagnostic radiologic examination; Drug Metabolic Detoxication; Elements; Environment; Epithelial; Etiology; Evaluation; Event; Excision; Experimental Designs; Failure; Female; Film; Friction; Generations; Gingiva; Gingival Crevicular Fluid; Goals; Growth; Histology; Immunohistochemistry; Impaired wound healing; Impairment; Implant; In Vitro; Incidence; Infection prevention; Inflammation; Inflammation Mediators; Inflammatory Response; Interruption; Kinetics; Liquid substance; Lubricants; Lubrication; Maxilla; Measurement; Measures; Methods; Microbial Biofilms; Modeling; Modification; Operative Surgical Procedures; Oral; Osseointegration; Outcome; Oxides; Performance; Perioperative; Phase; Postoperative Period; Process; Property; Race; Rattus; Reporting; Research; Research Personnel; Saliva; Series; Stress; Surface; Surface Properties; Techniques; Technology; Testing; Time; Tissues; Titanium; Torque; Toxic effect; Trauma; antimicrobial; base; biomaterial compatibility; biomineralization; bone; bone loss; cytotoxicity; design; experimental study; healing; implant design; implant material; implantable device; implantation; improved; in vivo; in vivo evaluation; liquid formulation; male; microbial; microbial colonization; microbial community; microbial host; microbiome; novel; oral condition; peri-implantitis; periodontopathogen; premature; preservation; prevent; recruit; response; seal; soft tissue; stem cell differentiation; success; surface coating; titanium dioxide

Multifunctional Ionic liquids Coatings for Dental Implant Surfaces Abstract Titanium dental implants are known for their high success rates and adequate osseointegration in vivo. However, with an increasing number of implants used, higher incidence of implant complications and failures have been recently reported. Implant failure is classified either as early or late stage failure. Early stage failure occurs when osseointegration is not achieved typically due to bacterial contamination, premature loading, excessive surgical trauma and impaired healing. Late stage failure occurs when implant osseointegration is lost having common etiological factors associated with bacterial-induced bone loss causing peri-implantitis, and excessive occlusal stresses. Recently, corrosion has also been considered a phenomenon underlying surface integration and performance. Bacteria seem to be key players in both early and late stage failures, with their presence influencing the establishment and loss of osseointegration. Hence, the success or failure of a dental implant can be related to its surface integration with soft and bone tissues versus biofilm adhesion. Early bacterial colonizers forming biofilms can impair soft tissue sealing by infiltrating and interrupting the process of surface integration. Current surface treatment techniques for dental implants typically aim to improve only one aspect of the problem, such as prevention of infection, promotion of osseointegration, or corrosion protection. However, to improve implant function, it is crucial to prevent early bacterial adhesion and to promote a permissive environment for tissue integration. In order to achieve multi-functionalities on implant surfaces, the goal of this proposal is to develop a new generation of coatings using ionic liquid (IL) technology. Non-toxic dicationic imidazolium-based IL coatings were designed to confer the surface of dental implants with: (i) antimicrobial activity for mitigation of biofilm adhesion, which will enable host cells to reach and seal the surface of the implant; (ii) protection of the oxide layer at the critical initial healing phase of the implant; and (iii) improved frictional properties for implant insertion. Aim 1 will study the competition of bacteria and host cells for the surface (“race for the surface”) of IL coated versus non-coated titanium using a co-culture approach. A co-culture model will be developed with conditions of varying concentrations of host and bacterial cells to test both peri-operative and post-operative models. In Aim 2, implants coated with the best-performing IL, as verified in Aim 1, will be investigated in an animal model. The effect of IL coating on the associated inflammatory response, soft and bone tissues, osseointegration, and bacterial load will be assessed at different time points simulating early and late healing periods. In vivo testing will enable observation of inflammatory responses and kinetics of bone growth triggered by the presence of IL- coated surfaces in comparison to non-coated implants. This new generation of coatings aims to mitigate bacteria adhesion on the surface following implantation while providing suitable surface conditions for tissue integration. Considering current surface treatments available, the proposed IL-coating may constitute a more potent strategy to improve dental implant surfaces.

牙科植入物表面的多功能离子液体涂料 抽象的 钛牙染色以其高成功率和体内足够的骨整合而闻名。然而， 随着越来越多使用的即将发生的汇编和失败的越来越多的事件 最近报道。植入物失败分为早期或晚期失败。早期失败会发生 通常由于细菌污染，过早负荷，多余的手术而无法实现骨整合。 创伤和治愈受损。当植入骨整合失去常见时，会发生晚期失败 与细菌诱导的骨质流失相关的病因因素，导致植入周围炎和过量的咬合 压力。最近，腐蚀也被认为是一种基础表面整合的现象 表现。细菌似乎是早期和晚期失败的关键参与者，并且存在着影响 骨整合的建立和损失。因此，牙科植入物的成功或失败可能是相关的 它与柔软和骨组织与生物膜粘合剂的表面整合。早期细菌殖民者形成 生物膜可以通过渗入和中断表面整合过程来损害软组织密封。当前的 牙科牙齿的表面处理技术通常只旨在改善问题的一个方面，例如 作为预防感染，促进骨整合或腐蚀保护。但是，要改善植入物 功能，这对于防止早期细菌粘附并促进组织的允许环境至关重要 为了在植入物表面上实现多功能性，该建议的目标是开发一个 使用离子液体（IL）技术的新一代涂料。无毒的基于咪唑的基于IL涂料 旨在赋予牙齿侵入表面：（i）抗菌活性以减轻生物膜 粘附，这将使宿主细胞能够到达并密封植入物的表面； （ii）保护氧化物 在植入物的临界初始愈合阶段的层； （iii）改善植入物插入的摩擦特性。 AIM 1将研究IL涂层的细菌和宿主细胞的竞争（“表面种族”） 使用共培养方法与非涂层钛相比。将开发一个共同培养模型的条件 不同浓度的宿主和细菌细胞测试围手术和术后模型。目标 2，将在AIM 1中验证的最佳性IL涂层的覆盖物将在动物模型中进行研究。这 IL涂层对相关炎症反应，软和骨组织，骨整合和 细菌负荷将在模拟早期和晚期愈合期的不同时间点进行评估。体内测试 将能够观察到存在IL-触发的炎症反应和动力学 与未涂层的玻璃杯相比，涂层表面。这种新一代涂料旨在减轻细菌 植入后表面上的粘附，同时为组织整合提供合适的表面条件。 考虑到当前的表面处理，拟议的IL涂层可能构成更潜在的策略 改善牙科植入物表面。