Multifunction High Throughput System for Neurophysiology of Behavior

行为神经生理学多功能高通量系统

• 批准号: 8781844
• 负责人: DONALD JAY WOODWARD
• 金额: $ 49.99万
• 依托单位: BIOGRAPHICS, INC.
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-15 至 2017-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8781844
• 关键词: Adopted; Adoption; Alcohol abuse; Alcohol consumption; Alcohol dependence; Alcoholism; Alcohols; Amplifiers; Animal Behavior; Animals; Behavior; Behavior Control; Behavioral; Behavioral Research; Brain; Brain region; Chemical Stimulation; Chemicals; China; Client; Cognition; Collection; Communication; Complex; Computer software; Computers; Data; Data Analyses; Data Set; Detection; Development; Devices; Disease; Dopamine; Drug Addiction; Drug Evaluation; Drug abuse; Drug effect disorder; Electrochemistry; Electronics; Equipment; Ethanol; Evaluation Research; Evolution; Family; Funding; Future; Genetic Models; Goals; Head; Housing; Image; Infusion Pumps; Infusion procedures; Ingestion; Intake; Intravenous infusion procedures; Investments; Laboratories; Lasers; Lead; Life; Light; Liquid substance; Measurement; Measures; Memory; Microdialysis; Mission; Motor; Movement; Mus; National Institute of Drug Abuse; National Institute of Mental Health; National Institute of Neurological Disorders and Stroke; National Institute on Alcohol Abuse and Alcoholism; Neurons; Neurosciences; Optics; Pain; Performance; Peripheral; Pharmaceutical Preparations; Phase; Phenotype; Physiologic pulse; Population; Procedures; Psyche structure; Pump; Rattus; Research; Research Activity; Rodent; Scanning; Schizophrenia; Seizures; Self Administration; Services; Signal Transduction; Site; Sleep; Small Business Innovation Research Grant; Source; Speed; Staging; Stimulus; Syringes; System; Systems Development; Technology; Testing; Therapeutic; Therapeutic Agents; Time; Tissues; Tube; Wireless Technology; Work; advanced system; aging brain; alcohol measurement; alcohol research; alcohol testing; analog; base; carbon fiber; commercialization; cost effective; cost effectiveness; data acquisition; design; digital; dopaminergic neuron; drinking; drug of abuse; experience; improved; innovation; instrument; instrumentation; mesolimbic system; miniaturize; neurophysiology; new technology; novel; parallel processing; prototype; public health relevance; research study; response; sensor; sensory gating; subcutaneous; validation studies

DESCRIPTION (provided by applicant): The goal of this project is to create a system to conduct research with the assistance of a 'High Throughput Multifunction System for Neurophysiology of Behavior'. This Phase II application to the Omnibus Solicitation is based on the Phase I for PA-08-001 that was funded by NIAAA 5R43AA020676-02. The advanced system will provide capabilities for many other Behavioral Neurophysiology research activity related to the missions of NIAAA, NIMH, NINDS, NIDA, and NIA. Multiple new technologies will be integrated to achieve a high performance high throughput system so that single operator can conduct multiple experiments. The main focus is to design and assemble an advanced data acquisition and control module for multiple continuous long duration studies of animals living in an experimental chamber. A rotating platform with tethered instrumentation will follow the movements of the animal. The purpose of the arrangement is to avoid the need for multiple swivel connection that limits the connections of different types that can be made with a moving animal. This arrangement will allow multiple electrical, optical and fluid connections to be made over long times. Installation of laser light sources and syringe infusion pumps on the rotating platform is a novel design. An advance data acquisition electronics module for recording and stimulation from a large number of channels will consist of an Altera Arria V GX FPGA with a PCIe connection to a PC motherboard. The multiple functions will allow continuous analog signal recording spike waveform capture, simultaneously electrochemical detection from many channels. The module also includes extensive capabilities for multiple imager support. The device is re-purposed from a DARPA that supported 'Gigapixel' camera project to enable parallel processing from hundreds of imager devices. The unique design will enable assembly of very large system configurations 10,000 or more parallel channels for future neuroscience as related technology matures. The compact unit will be installed above an experimental chamber on a unit that will rotate by a motor system triggered by movement of the rodent. The rotating system has been designed for transfer of power and control. High speed wireless, optical, or digital communication from the rotating unit will allow data and control signals to pass from each experimental chamber to a host computer data acquisition service A further aim is to advance a universal real time state machine for control of behavioral chambers and multiple devices for sensing and stimulation. Remote control client software will be created for control and analysis of data. An additional aim is to conduct evaluation studies of drugs related to therapy for multiple applications in behavioral neurophysiology related to development of therapeutic agents. Neuron population activity and multiple carbon fibers will employ fast scan electrochemistry to detect dopamine release transient in multiple brain regions with rats housed continuously over thirty days while testing responses. Microdialysis probes, multiple precision syringe pumps, camera imaging, and a colorimetric system will be adapted for repeated measurement of tissue alcohol levels in rats ingesting ethanol over time with intermittent access. Efficiency of microdialysis research will be enhanced by allowing use of multiple probes along with an improved automated collection system. A group of Collaborators to validate will provide critical and essential tests and evaluation for research applications in alcohol and drug addiction, schizophrenia, aging brain, sleep and brain states, pain modulation, and seizure prediction. The overall goal of the introduction of the interrelated enhancements is to reduce the required labor and instrumentation to achieve high efficiency for many types of behavioral research conducted simultaneously in multiple chambers, while employing a family of sensors and technologies that will be adapted for use with the rotating system. By achieving a high level of integration and efficiency the system aims to result in a System for Development of Therapeutic Drugs and Procedures'

描述（由申请人提供）：该项目的目标是创建一个系统，在“行为神经生理学高通量多功能系统”的帮助下进行研究。综合征集的第二阶段申请基于 PA-08-001 的第一阶段，该阶段由 NIAAA 5R43AA020676-02 资助。该先进系统将为与 NIAAA、NIMH、NINDS、NIDA 和 NIA 任务相关的许多其他行为神经生理学研究活动提供功能。将集成多种新技术以实现高性能高通量系统，以便单个操作员可以进行多个实验。 主要重点是设计和组装先进的数据采集和控制模块，用于对实验室中的动物进行多次连续长时间的研究。带有系留仪器的旋转平台将跟随动物的运动。该布置的目的是避免需要多个旋转连接，这限制了可以与移动的动物进行的不同类型的连接。这种布置将允许长时间建立多个电、光和流体连接。在旋转平台上安装激光光源和注射输液泵是一种新颖的设计。 用于记录和刺激大量通道的先进数据采集电子模块将由 Altera Arria V GX FPGA 组成，并通过 PCIe 连接到 PC 主板。多种功能将允许连续模拟信号记录尖峰波形捕获，同时从多个通道进行电化学检测。该模块还包括支持多个成像仪的广泛功能。该设备由支持“十亿像素”相机项目的 DARPA 重新设计，可实现数百个成像设备的并行处理。随着相关技术的成熟，独特的设计将能够为未来的神经科学组装具有 10,000 个或更多并行通道的超大型系统配置。该紧凑型装置将安装在实验室上方的一个装置上，该装置将由啮齿动物的运动触发的电机系统旋转。 旋转系统专为传输动力和控制而设计。来自旋转单元的高速无线、光学或数字通信将允许数据和控制信号从每个实验室传递到主机数据采集服务。进一步的目标是推进通用实时状态机，用于控制行为室和多个用于传感和刺激的设备。将创建远程控制客户端软件来控制和分析数据。 另一个目的是对与治疗相关的药物进行评估研究，以用于与治疗剂开发相关的行为神经生理学中的多种应用。神经元群活动和多个碳纤维将采用快速扫描电化学来检测多个大脑区域的多巴胺释放瞬态，将大鼠连续饲养三十天以上，同时测试反应。微透析探针、多个精密注射泵、相机成像和比色系统将用于重复测量间歇性摄入乙醇的大鼠的组织酒精水平。通过允许使用多个探针以及改进的自动收集系统，微透析研究的效率将得到提高。一组进行验证的合作者将为酒精和药物成瘾、精神分裂症、大脑老化、睡眠和大脑状态、疼痛调节和癫痫发作预测方面的研究应用提供关键和必要的测试和评估。 引入相关增强功能的总体目标是减少 需要劳动力和仪器来实现在多个室中同时进行的多种类型的行为研究的高效率，同时采用一系列适用于旋转系统的传感器和技术。通过实现高水平的集成和效率，该系统旨在形成治疗药物和程序开发系统