Effects of Developmental Alcohol Exposure on Astrocyte Ca2+ Signaling and Structural Synaptic Plasticity

发育期酒精暴露对星形胶质细胞 Ca2 信号传导和结构突触可塑性的影响

基本信息

批准号：
9761943
负责人：
Padmashri Ragunathan
金额：
$ 18万
依托单位：
UNIVERSITY OF NEBRASKA MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-01 至 2022-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9761943
关键词：
Affect Alcohols Animal Model Astrocytes Attention Behavioral Brain Brain region Child Dendritic Spines Development Exhibits Fetal Alcohol Exposure Fetal Alcohol Spectrum Disorder Forelimb Functional disorder Goals Image Impaired cognition Impairment Injections Label Language Development Learning Memory Modeling Monitor Motor Motor Cortex Motor Skills Mus Neuronal Plasticity Neurons Neuropsychology Pregnancy Process Reporting Role Signal Transduction Slice Structure Synapses Synaptic plasticity Testing Therapeutic Vertebral column Viral Vector Visuospatial alcohol exposure brain abnormalities design designer receptors exclusively activated by designer drugs executive function experience imaging modality improved in vivo in vivo imaging insight motor control motor learning motor skill learning mouse model prenatal exposure social therapeutic target

项目摘要

Abstract Exposure to alcohol during pregnancy produces fetal alcohol spectrum disorders (FASD) which is associated with cognitive impairments in various neuropsychological domains. Children with FASD often exhibit sensorimotor dysfunctions that include deficits in motor coordination and fine motor control. Although developmental alcohol exposure has been shown to affect astrocytes and cause impairments in neuronal plasticity in several brain regions, very little is known about alterations in astrocytic Ca2+ signaling and synaptic changes that occur in the intact brain. Here we will use prenatal exposure mouse model of fetal alcohol spectrum disorders to study alcohol-induced alterations in basal and motor-skill learning induced astrocytic Ca2+ signaling and structural synaptic plasticity. We will combine in vivo imaging of dendritic spines with astrocytic Ca2+ imaging under basal conditions and with learning. The goal of the proposal is to determine whether developmental alcohol exposure results in alterations in basal and motor-skill learning induced astrocytic Ca2+ signaling and structural synaptic plasticity.

抽象的怀孕期间接触酒精会导致胎儿酒精谱系障碍 (FASD) 与各种神经心理学领域的认知障碍有关。患有 FASD 的儿童经常表现出感觉运动功能障碍，包括运动协调和精细运动控制缺陷。尽管发育过程中的酒精暴露已被证明会影响星形胶质细胞并导致损伤在几个大脑区域的神经元可塑性中，我们对星形细胞 Ca2+ 的变化知之甚少完整大脑中发生的信号传导和突触变化。这里我们将使用产前暴露小鼠胎儿酒精谱系障碍模型，用于研究酒精引起的基础和运动技能的改变学习诱导星形细胞 Ca2+ 信号传导和结构突触可塑性。我们将结合体内在基础条件和学习条件下使用星形细胞 Ca2+ 成像对树突棘进行成像。这该提案的目标是确定发育时期的酒精暴露是否会导致基础和运动技能学习诱导星形细胞 Ca2+ 信号传导和结构突触可塑性。