Systems Neuroscience Imaging Resource

系统神经科学成像资源

基本信息

批准号：
9569204
负责人：
Theodore Usdin
金额：
$ 305.26万
依托单位：
NATIONAL INSTITUTE OF MENTAL HEALTH
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9569204
关键词：
4 year old Anatomy Antibodies Area Brain Brain Mapping Brain imaging Brain region Callithrix Collaborations Computer software Data Development Equipment Evaluation Gene Expression Genetic Histologic Hour Image Image Analysis In Situ Hybridization Investigation Label Laboratories Laser Scanning Confocal Microscopy Lasers Light Lighting Macaca Methods Microscope Microscopy Microtome - medical device Modernization Molecular Molecular Profiling Mus National Institute of Mental Health Neurons Neurosciences Nucleic Acid Probes Pattern Population Preparation Procedures Protocols documentation Research Personnel Research Project Grants Resolution Resources Scanning Slide Specific qualifier value System Systems Analysis Techniques Technology Thick Time Tissue imaging Tissues Training Support Work base brain tissue cell type cryostat detector equipment acquisition image processing instrument molecular imaging operation programs protein distribution pyridinium 3-methoxyestra-1,3,5(10)-trien-6-yl sulfate reconstruction student training tool

项目摘要

This is the first year of operation of the Systems Neuroscience Imaging Resource (SNIR). Much of the focus has been on equipment acquisition and procedure development. The first piece of equipment specifically purchased for the SNIR is a Zeiss AxioscanZ1 slide scanning microscope. This is a high quality widefield microscope with transmitted brightfield and fluorescent epi-illumination capacity. Its most significant feature is the ability to program multichannel tiled acquisition of large areas from up to 100 microscope slides. It is being actively used by investigators from 10 different intramural laboratories for projects that include whole brain mapping of gene expression profiles and the projections of genetically tagged and fluorescently labeled neuron populations. The SNIR has taken on management of a shared Zeiss LSM780 microscope. This instrument is approximately 4 years old. It is a high quality inverted confocal microscope with 405, 488, 514, 561, 594 and 633 nm lasers, a 32-channel spectral detector and 2 conventional PMTS. It is generally used to capacity during core working hours by investigators from approximately 10 different NIMH intramural groups. Acquisition of additional microscopes aimed at systems neuroscience and whole brain imaging is planned. These include an upright confocal/multiphoton microscope and a light sheet microscope. Renovation of space to accommodate this equipment is planned. Microbrightfield Brainmaker and Neurolucida 360 software packages have been acquired to facilitate reconstruction and analysis of systems neuroscience data. Additional software purchases are under investigation. Protocols for multiplex in situ hybridization are being developed and work toward implementation of established protocols for clearing and imaging thick pieces of brain tissue is underway.

这是系统神经科学成像资源（SNIR）操作的第一年。重点是设备采集和程序开发。专门为SNIR购买的设备是Zeiss Axioscanz1滑动扫描显微镜。这是一个高质量的广场显微镜，具有传播的Brightfield和荧光表演能力。它最重要的功能是能够编程多通道瓷砖从最高100个显微镜幻灯片进行大面积采集。来自10个不同壁内实验室的研究人员正在积极使用它，用于包括基因表达谱的整个大脑映射以及遗传标记和荧光标记神经元种群的投影。 SNIR已进行了共享的Zeiss LSM780显微镜的管理。该乐器大约有4年的历史。这是一个高质量的倒置共聚焦显微镜，具有405、488、514、561、594和633 nm激光器，一个32通道光谱探测器和2个常规PMT。通常，来自大约10个不同NIMH壁内组的研究人员在核心工作时间内将其用于容量。计划收集针对系统神经科学和整个大脑成像的其他显微镜。这些包括直立的共聚焦/多光子显微镜和光片显微镜。计划安装该设备的空间进行翻新。 Microbrightfield Brainmaker和Neurolucida 360软件包已获取，以促进系统神经科学数据的重建和分析。其他软件购买正在调查中。正在开发用于多重原位杂交的协议，并致力于实施已建立的方案清理和成像厚实的脑组织。