Spatiotemporal Control of Dynamic Notch Signaling with Subcellular Resolution

具有亚细胞分辨率的动态Notch信号传导的时空控制

基本信息

批准号：
9314590
负责人：
Young-wook Jun
金额：
$ 30.12万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-08-01 至 2019-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9314590
关键词：
Address Alpha Cell Atomic Force Microscopy Behavior Biological Process Calibration Cell Communication Cell Line Cell Nucleus Cell surface Cells Chemistry Critical Pathways Development Diagnostic Dimensions Disease Elements Endocytosis Event Exertion Genes Goals Hand Human Image Imagery In Situ In Vitro Individual Intracellular translocation Investigation Knowledge Label Ligands Location Lymphoma Magnetism Maintenance Malignant Neoplasms Measures Mechanics Mediating Methodology Methods Modeling Molecular Molecular Conformation Monitor Multiple Sclerosis Nanotechnology Normal tissue morphology Nucleic Acid Regulatory Sequences Oligonucleotides Optics Play Population Process Property Proteins Proteolysis Receptor Activation Receptor Signaling Regulation Resolution Role Signal Transduction Spatial Distribution Stem cells System Techniques Technology Therapeutic Time angiogenesis cell behavior design experimental study imaging capabilities innovation magnetic field nanoparticle nanoprobe nanosystems nerve stem cell neurodevelopment next generation notch protein plasmonics public health relevance receptor single molecule spatiotemporal stem cell therapy tool

项目摘要

DESCRIPTION (provided by applicant): Notch signaling is a highly conserved cell-to-cell communication mechanism, which plays a central role in defining individual cells' behaviors and fates during development. Despite rapidly increasing knowledge of these signaling events, little is known about how spatiotemporal dynamics of receptor signaling across the cell influence signal exchange. To address this challenge, we propose an advanced nanosystem that mimics, enabling simultaneous real-time monitoring and in situ regulation of Notch signaling in a particular cell at any desired location and time with subcellular resolution. Using this new nanotechnology, we first determine force-induced structural features of Notch receptors. We also explore Notch signaling in neural stem cells to determine how the spatiotemporal distribution of Notch signaling across a cell population influences the final fate of individual cels during development. Binary cell communication via Notch.

描述（由申请人提供）：Notch信号传导是一种高度保守的细胞间通信机制，在定义单个细胞在发育过程中的行为和命运方面起着核心作用。尽管对这些信号事件的了解迅速增加了，但对细胞影响信号交换的受体信号传导的时空动力学方式知之甚少。为了应对这一挑战，我们提出了一个高级纳米系统，该系统可以模仿模仿，从而可以在任何所需的位置和亚细胞分辨率下同时进行实时监视和原位调节Notch信号传导。使用这种新的纳米技术，我们首先确定力引起的Notch受体的结构特征。我们还探索了神经干细胞中的Notch信号传导，以确定Notch信号在细胞种群中的时空分布如何影响发育过程中单个CEL的最终命运。通过Notch进行二元电池通信。