Characterization of pmoD

pmoD 的表征

基本信息

批准号：
9278969
负责人：
Oriana S Fisher
金额：
$ 5.71万
依托单位：
NORTHWESTERN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-07-01 至 2019-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9278969
关键词：
Active Sites Address Affinity Ammonia Bacteria Binding Biochemical Biochemistry Biological Biological Assay Biological Process C-terminal Carbon Carbon Dioxide Cell physiology Chemicals Copper Crystallization Data Environment Exhibits Formaldehyde Genes Global Warming Goals Gram-Negative Aerobic Bacteria Growth Immunoprecipitation Industrialization Iron Knock-out Laboratories Length Link Medicine Membrane Membrane Proteins Metabolism Metal Ion Binding Metals Methane Methane Metabolism Pathway Methane hydroxylase Methanol Methylosinus trichosporium Mixed Function Oxygenases Modernization Molecular Multienzyme Complexes Operon Organism Particulate Pathway interactions Pattern Phenotype Process Production Proteins Protomer Reaction Regulation Research Role Series Source Starvation Structure Testing Time Work base biophysical properties cell growth college experimental study greenhouse gases in vivo insight oxidation periplasm public health relevance tool uptake

项目摘要

DESCRIPTION (provided by applicant): Methane is a prevalent greenhouse gas that contributes to global warming. While synthetically it is difficult to chemically convert methane to less environmentally harmful substances, methanotrophic bacteria have developed an efficient way to metabolize methane. In the first step of the process, methanotrophs use a copper dependent enzyme complex called particulate methane monooxygenase (pMMO) to convert methane to methanol, but precisely how this occurs biochemically and how pMMO may be regulated are not well understood. The primary goal of this proposal is to characterize a protein called pmoD that is also likely involved in methane conversion and may somehow regulate pMMO. In one component of this work the phenotype of pmoD will be studied using a pmoD knockout strain. A second aspect of the work will focus on structural, biochemical, and spectroscopic characterization of pmoD. The third aim addresses the function of pmoD by determining whether it can interact with other proteins involved in copper metabolism and methane conversion and whether it can regulate these processes. Together, this combination of in'vivo studies, structural characterization, and biochemical analysis is expected to provide a comprehensive description of pmoD and its roles in methane metabolism and copper regulation. The insights gained from these studies have the potential to greatly enhance the current understanding of biological methane oxidation. Biochemical analysis is expected to provide a comprehensive description of pmoD and its roles in methane metabolism and copper regulation. The insights gained from these studies have the potential to greatly enhance the current understanding of biological methane oxidation.

描述（由适用提供）：甲烷是一种普遍有助于全球变暖的温室气体。虽然合成的是很难将甲烷化学转化为甲烷营养细菌对环境有害的物质较少，已经开发了一种代谢甲烷的有效方法。在该过程的第一步中，甲烷嗜酸菌使用称为特定的甲烷单加氧酶（PMMO）的铜依赖性酶复合物将甲烷转化为甲醇，但恰恰是如何在生化上发生这种情况以及如何调节PMMO。该提案的主要目标是表征一种称为PMOD的蛋白质，该蛋白质也可能参与甲烷转化率，并可能以某种方式调节PMMO。在这项工作的一个组成部分中，PMOD的表型将使用PMOD敲除菌株进行研究。工作的第二个方面将集中于PMOD的结构，生化和光谱表征。第三个目的通过确定是否可以与参与铜代谢和甲烷转化的其他蛋白质相互作用以及是否可以调节这些过程来解决PMOD的功能。总之，预期在In'Vivo研究，结构表征和生化分析的结合中，可以全面描述PMOD及其在甲烷代谢和铜调节中的作用。从这些研究中获得的见解有可能大大增强对生物代谢的当前理解。预计生化分析将对PMOD及其在甲烷代谢和铜调节中的作用提供全面描述。从这些研究中获得的见解有可能大大增强对生物甲烷氧化的当前理解。