Center on Membrane Protein Production and Analysis (COMPPAA)

膜蛋白生产和分析中心 (COMPPAA)

基本信息

批准号：
9268638
负责人：
WAYNE A. HENDRICKSON
金额：
$ 144.21万
依托单位：
NEW YORK STRUCTURAL BIOLOGY CENTER
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-05-01 至 2021-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9268638
关键词：
Address Automobile Driving Beds Biochemical Biochemistry Biological Assay Biology Biomedical Technology Cells Cellular biology Chimeric Proteins Collaborations Country Cryoelectron Microscopy Crystallization Databases Deposition Detergents Development Disease Educational workshop Electrophysiology (science)Environment Escherichia coli FDA approved Fluorescence Generic Drugs Genomics Health Human Human Resources Individual Institutes International Kinetics Label Life Maps Medicine Membrane Membrane Proteins Modeling Molecular Chaperones Molecular Target Mutation NMR Spectroscopy New York Organelles Pharmaceutical Preparations Procedures Process Production Property Proteins Publications Radiation induced damage Radioactive Reagent Recombinants Research Personnel Resources Sampling Services Signal Transduction Structural Biologist Structure Talents Technology Testing Training Training Activity Training Programs Variant X-Ray Crystallography base drug discovery genetic selection high throughput screening meetings minority student new technology novel therapeutics programs protein complex protein function protein structure research and development screening structural biology uptake web site

项目摘要

DESCRIPTION (provided by applicant): Proteins in membranes provide the portals through which membrane-delimited organelles within cells and cells themselves communicate with their external environments. These essential properties make membrane proteins crucial components in biochemistry and in cell biology. Many membrane proteins are involved in processes of disease, and they are the molecular targets of over 40% of all FDA-approved drugs. Atomic level structure informs both biochemical understanding and drug discovery. Yet, whereas membrane proteins constitute 20-30% of all proteins in all of life, results from membrane proteins comprise only 1.6% of atomic structures in the Protein Data Bank. To a large extent, this structural under-representation arises because of the challenges in producing and analyzing membrane protein structure and function. New technology is needed to address these challenges. We propose to develop the Center on Membrane Protein Production and Analysis (COMPPAA, or COMPPÅ for short) as a Biomedical Technology Research Resource (BTRR) for the production and study of membrane proteins of relevance to biology and medicine. We will build on existing facilities and talents at the New York Structural Biology Center (NYSBC), which include capabilities for structure determination by x-ray crystallography, cryo-electron microscopy (cryo-EM) and NMR spectroscopy as well as a facility for high-throughput expression testing and detergent screening for membrane proteins. COMPPÅ will have nine components including four Technology Research and Development (TR&D) projects which will be driven by Driving Biomedical Project (DBP) component that will comprise fifteen selected DBPs to serve as test beds for the TR&D development. TR&D1 will develop technology for the efficient production of recombinant membrane proteins. TR&D2 will develop technology for the optimization of membrane protein samples for structural analysis. TR&D3 will develop generic assays for functional analy

描述（通过应用程序证明）：膜中的蛋白质与它们的环境进行交流的门户。所有批准的药物。出现在产生和分析膜蛋白结构和功能方面的挑战，以解决这些挑战，以开发膜蛋白拱门资源（BTRR）中心。与生物学和医学相关。洗涤剂屏幕桥蛋白将有9个组件，包括四个技术研发（TR＆D）项目，CH将推动生物医学项目（DBP）组件，该项目将构成Fiomedisen选择的DBP开发二聚膜蛋白的技术。