SBIR Topic 359 Phase I: Highly-Efficient Isolation and Fidelity Differentiation of Exosomes (HIFiDE) from Bodily Fluids Kit

SBIR 主题 359 第一阶段：体液试剂盒中外泌体 (HIFiDE) 的高效分离和保真分化

• 批准号: 9570532
• 负责人: Donald Johnson
• 金额: $ 30万
• 依托单位: CHROMOLOGIC, LLC
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-15 至 2018-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9570532
• 关键词: Cell Line; Cells; Coupled; Development; Disease; Encapsulated; Inflammatory; Integral Membrane Protein; Ion Exchange; Liquid substance; Methods; Peptides; Phase; Plant Resins; Population; Process; Signal Transduction; Small Business Innovation Research Grant; Therapeutic; cancer cell; cell type; exosome; interest; protein profiling; transcriptome

Exosomes encapsulate and transport a wide variety of molecules generated by their cell-of-origin, a process now thought to be a form of cellular signaling. Exosome signaling is common across cell types and species, but it is of particular interest in diseases with an inflammatory component. While exosome isolation and analysis is useful to understanding the mechanisms behind these multifaceted diseases, exosomes may also be exploited for their therapeutic potential. In particular, exosomes derived from healthy cells have been found to effectively alter the transcriptome of cancer cells. Nonetheless, there are no methods for differentiating between healthy- and cancer-cell derived exosomes in bodily fluids. In this project, we aim to develop a method for isolating exosomes and oncosomes, coupled with a method for the differentiation of exosome populations.

外泌体封装和转运其原细胞产生的各种分子，这一过程现在被认为是细胞信号传导的一种形式。外部信号在细胞类型和物种之间很常见，但在具有炎症成分的疾病中特别感兴趣。虽然外泌体隔离和分析对于理解这些多方面疾病背后的机制很有用，但外泌体也可能因其治疗潜力而被利用。特别是，已经发现源自健康细胞的外泌体有效地改变了癌细胞的转录组。尽管如此，没有方法可以区分身体液中的健康和癌细胞衍生的外泌体。在这个项目中，我们旨在开发一种隔离外泌体和癌症的方法，再加上一种分化外泌体种群的方法。