Core E

核心E

• 批准号: 8879400
• 负责人: GARNETT H KELSOE
• 金额: $ 19.16万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8879400
• 关键词: Affinity; Antibody Affinity; Antigen-Antibody Complex; Antigens; B cell repertoire; B-Lymphocytes; Biotechnology; Case Study; Cell Culture System; Cell Lineage; Cells; Data; Development; Epitopes; Generations; Hemagglutinin; Human; Immunity; Immunization; Infection; Influenza; Joints; Maps; Modeling; Mus; Mutation; Names; Nature; Population; Reaction; Resolution; Seasons; Structure; Structure of germinal center of lymph node; Surface; Testing; Vaccines; Variant; Virus; Work; base; design; flu; immunogenic; influenza virus vaccine; influenzavirus; mouse model; neutralizing antibody; novel; pathogen; response; stem; transmission process; vaccine development

We have added Core E, to support a new joint Aim of the POI, described in both in the plans for the coming year in Project 3 and more briefly in Project 1. Dr. Kelsoe, the Pi of this Core, has been working closely with Haynes, Liao, and Harrison, and he is a coauthor (with Haynes, Harrison, and Kepler) ofthe review that describes (and names) B-cell lineage based immunogen design (Haynes, B.F., Kelsoe, G., Harrison, S.C, Kepler, T.B. B-cell-lineage immunogen design in vaccine development with HlV-1 as a case study. Nature Biotechnology 10: 423 (2012), PMC3512202). The novel, single-cell, B-cell culture system will allow us to test this notion on a feasible timescale in a tractable mouse model, and it will yield data that should facilitate more realistic models of the germinal center reaction and antibody affinity maturation (in humans as well as in mice) in the context of an influenza virus immunogen.

我们添加了 Core E，以支持 POI 的新联合目标，这在未来的计划中都有描述 在项目 3 中进行了一年的研究，在项目 1 中进行了更简短的介绍。Kelsoe 博士是该核心的 Pi，一直与 海恩斯、廖和哈里森，他是该评论的合著者（与海恩斯、哈里森和开普勒） 描述（并命名）基于 B 细胞谱系的免疫原设计（Haynes, B.F.、Kelsoe, G.、Harrison, S.C.、 开普勒，T.B.以 HIV-1 为例的疫苗开发中的 B 细胞谱系免疫原设计作为案例研究。自然 生物技术 10：423（2012），PMC3512202）。新颖的单细胞 B 细胞培养系统将使我们能够 在易驯服的小鼠模型中在可行的时间尺度上测试这个概念，它将产生应该促进的数据 更真实的生发中心反应和抗体亲和力成熟模型（在人类以及 在小鼠中）在流感病毒免疫原的背景下。