Polysaccharide-Polyamine Hydrogels

多糖-聚胺水凝胶

• 批准号: 9210629
• 负责人: Naphtali Andre O'Connor
• 金额: $ 11.84万
• 依托单位: HERBERT H. LEHMAN COLLEGE
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-05-01 至 2019-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9210629
• 关键词: Affect; Amination; Amines; Ammonium; Anti-Inflammatory Agents; Anti-inflammatory; Antioxidants; Biological Assay; Biological Sciences; Biology; Bypass; Carbohydrates; Cations; Cell Adhesion; Cell Proliferation; Cell-Matrix Junction; Charge; Chemicals; Collaborations; Contact Lenses; Curcumin; Development; Dextrans; Dimensions; Drug Delivery Systems; Effectiveness; Engineering; Growth Factor; Hydrogels; Imines; In Vitro; Malignant Neoplasms; Methodology; Methods; Modification; Peptides; Polyamines; Polyethylenes; Polylysine; Polymers; Polysaccharides; Process; Speed; Sterile coverings; Stimulus; Therapeutic; Tissue Engineering; Toxic effect; Universities; VEGFA gene; Vitamins; Water; Work; Wound Healing; adduct; amino group; amphiphilicity; angiogenesis; antimicrobial; base; biological adaptation to stress; cell motility; college; controlled release; crosslink; healing; in vivo; neovascularization; novel; polyallylamine; polyphenol; public health relevance; scaffold; success; vasculogenesis; wound

 DESCRIPTION (provided by applicant): Hydrogels are three-dimensional networked polymers capable of absorbing and retaining copious quantities of water. Recently, hydrogels have been prominently featured as stimuli responsive materials for drug delivery and as scaffolds in tissue engineering. Commercially, they are viable and integral components in various materials, including contact lens and wound dressings. A recent study by Gerecht and coworkers has shown an amine functionalized dextran hydrogel to speed angiogenesis and neovascularization without the use of additives like grow factors or vitamins. We have recently developed a facile universal 1-step approach to incorporate polysaccharides and polyamines into hydrogels. Using this methodology, we propose a study of how the polysaccharide and amine affects wound healing. The hydrogels are to be evaluated by in vitro methods, using cell migration and proliferation assays. Polysaccharides with varying comprising carbohydrates will be examined along with varying polyamines and will be compared against the Gerecht polymer. A curcumin based polyamine has also been prepared and its effect on wound healing will be examined. Curcumin is an anti-oxidant, anti-inflammatory polyphenol and has shown success as a wound healing therapeutic in both in vitro and in vivo wound healing studies. Curcumin has been shown to induce a stress response and results in the hermetic induction of wound healing. We have prepared a biodegradable curcumin polyamine polymer and have integrated it into a hydrogel using our developed methodology and will examine these materials for their wound healing ability. Curcumin also has activity against a broad array of cancers and this hydrogel will also be examined as a cancer therapeutic. Keywords: Hydrogels, antimicrobial, wound healing, cancer

 描述（由申请人提供）：水凝胶是能够吸收和保留大量水的三维网络聚合物，最近，水凝胶已被广泛用作药物递送的刺激响应材料和组织工程中的支架，在商业上是可行的。 Gerecht 及其同事最近的一项研究表明，胺功能化的葡聚糖水凝胶可以加速血管生成和新血管形成，而无需使用我们最近开发了一种简单的通用一步法，将多糖和多胺掺入水凝胶中，我们建议研究多糖和胺如何影响伤口愈合。通过体外方法，使用细胞迁移和增殖测定，将与不同的多胺一起检查含有不同碳水化合物的多糖，并将其与 Gerecht 聚合物姜黄素进行比较。姜黄素是一种抗氧化、抗炎多酚，并且在体外和体内伤口愈合研究中都显示出作为伤口愈合治疗剂的成功。研究表明，它可以诱导应激反应，并导致伤口愈合的密封性。我们制备了一种可生物降解的姜黄素聚胺聚合物，并使用我们开发的方法将其整合到水凝胶中，并将检查这些材料的姜黄素伤口愈合能力。还具有对抗多种癌症的活性，这种水凝胶将 也可作为癌症治疗剂进行检查 关键词：水凝胶、抗菌、伤口愈合、癌症。