BSL3 Core

BSL3 核心

• 批准号: 9322140
• 负责人: Lucio Gama
• 金额: $ 28.88万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-06-23 至 2022-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9322140
• 关键词: Aftercare; Aliquot; Animal Model; Animals; Archives; Autopsy; Biological; Biological Markers; Blood; Blood specimen; Brain; Bronchoalveolar Lavage; CD4 Positive T Lymphocytes; Cell Line; Cells; Communication; Complete Blood Count; Consult; Data; Data Quality; Databases; Documentation; Environment; Equipment; Equipment and supply inventories; Flow Cytometry; Freezing; Genome; Goals; HIV; In Vitro; Individual; Laboratories; Leukocytes; Lung; Macaca; Manuscripts; Measures; Methods; Modeling; Monitor; Nucleic Acids; Peripheral Blood Mononuclear Cell; Plasma; Population; Preparation; Primary Cell Cultures; Procedures; Process; Productivity; Reporting; Research Personnel; Research Project Grants; Rest; Role; SIV; Sampling; Specialist; Specimen; Spleen; Supervision; Techniques; Technology; Tissues; Training; Update; Viral Load result; Virus; Withholding Treatment; Work; antiretroviral therapy; experimental study; in vivo; lymph nodes; macrophage; member; viral rebound; wasting

BSL-3 CORE - ABSTRACT The overall hypothesis for this PPG application is that both resting CD4+ T cells and resident macrophages harbor latent SIV genomes during longterm antiretroviral therapy (ART), and therefore contribute to the rebound of virus in blood, CSF, and tissues after ART cessation. The BSL-3 Core is a pivotal part of this proposal since all four research projects will utilize samples collected from infected macaques. To support this overall PPG, we will: A) Quickly execute targeted measures data for each project and communicate it rapidly to investigators; consult and communicate daily with team members of all of the individual projects to provide support on sample preparation, quantitation of biological parameters, and data dissemination; B) Work hand in hand with the Animal Core to isolate the appropriate samples and prepare them in a manner for subsequent analysis with the appropriate methods; C) Continually update project specific and overall databases and provide coherent data summaries and figures suitable for inclusion into manuscripts to facilitate data dissemination and communication; D) Provide access to cutting edge technologies to support projects and identify and apply technologies to streamline analysis and increase productivity and data quality.

BSL -3核心 - 摘要 该PPG应用的总体假设是静止的CD4+ T细胞和常驻巨噬细胞 长期抗逆转录病毒疗法（ART）期间，潜在的SIV基因组有助于 停止在血液，CSF和组织中病毒的反弹。 BSL-3核心是该提案的关键部分，因为所有四个研究项目都将利用收集的样本 来自被感染的猕猴。为了支持这一整体PPG，我们将： a）快速执行每个项目的目标量度数据，并迅速将其传达给研究人员； 每天与所有各个项目的团队成员咨询并进行沟通，以提供支持 样品制备，生物学参数的定量和数据传播； b）与动物核心手工合作，以分离适当的样品并以某种方式准备 以适当的方法进行后续分析； c）不断更新项目和整体数据库，并提供连贯的数据摘要和数字 适合纳入手稿，以促进数据传播和通信； d）提供对尖端技术的访问，以支持项目并识别和应用技术 简化分析并提高生产率和数据质量。