Control of C. elegans lineage by heterochronic genes

异时基因对线虫谱系的控制

基本信息

批准号：
9234684
负责人：
GARY B RUVKUN
金额：
$ 64.81万
依托单位：
MASSACHUSETTS GENERAL HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
1991
资助国家：
美国
起止时间：
1991-05-01 至 2020-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9234684
关键词：
Ablation Affect Alleles Animals Arabidopsis Bacteria Biological Biological Assay Biology Biotin Biotinylation Caenorhabditis elegans Candidate Disease Gene Chimeric Proteins Clustered Regularly Interspaced Short Palindromic Repeats Code Collection Complex Coupled Cytoplasmic Granules Defect Detection Disease Double-Stranded RNA Down-Regulation Engineering Enzymes Escherichia coli Exons Gene Duplication Gene Expression Gene Silencing Gene Targeting Genes Genetic Screening Genetic screening method Genome Heart Diseases Introns Malignant Neoplasms Mediating Messenger RNA MicroRNAs Modification Molecular Profiling Monitor Mutation Pathway Analysis Pathway interactions Pattern Phalanx Phenotype Phylogenetic Analysis Polymerase Production Proteins RNA Interference RNA Sequences RNA Splicing RNA amplification RNA interference screen RNA-Directed RNA Polymerase Reagent Regulation Repression Rest Role Signal Transduction Small Interfering RNA Small RNA Streptavidin Sulfhydryl Compounds Surveys Testing Tissues Transfer RNA Virus base cofactor crosslink crosslinking and immunoprecipitation sequencing drug development duplicate genes fusion gene gene function genetic analysis helicase mRNA Transcript Degradation mutant response trafficking transcription factor transcriptome sequencing

项目摘要

Project Summary/Abstract: We have identified a number of genes via genetic analysis and RNA interference gene inactivations that act as protein coding cofactors for the function of miRNAs and siRNAs in C. elegans. Some of these proteins were identified in genetic screens for decrease in miRNA function, some in genetic screens for decrease in siRNA function, and some in genetic screens for increase in siRNA function. We have also identified the target small RNAs that mediate these functions by deep RNA sequencing of selected mutant strains. We propose to dissect in detail how these proteins orchestrate the production, trafficking, and function of small RNAs in both mRNA degradation, mRNA translational control, and control of gene expression. We also propose to discern how the miRNA and siRNA and other small RNA pathways may compete with each other for common cofactors, thus leading to an increase in function in one pathway, when the other pathway is debilitated.

项目摘要/摘要：我们已经通过遗传分析和RNA干扰基因失活来鉴定了许多基因作为蛋白质编码的辅助因子，用于秀丽隐杆线虫中miRNA和siRNA的功能。其中一些蛋白质在遗传筛选中鉴定出miRNA功能的降低，其中一些在遗传筛选中以减少 siRNA功能，有些在遗传筛选中增加了siRNA功能。我们还确定了靶向小的RNA，通过对选定突变菌株的深度RNA测序介导这些功能。我们建议详细阐述这些蛋白质如何协调小的生产，运输和功能 mRNA降解，mRNA翻译对照和基因表达的控制中的RNA。我们也是提议辨别miRNA和siRNA以及其他小RNA途径如何相互竞争对于普通辅助因子，当另一种途径为衰弱。