Identifying non-coding RNAs for early detection and prevention of lung cancer

鉴定非编码 RNA 以早期检测和预防肺癌

基本信息

批准号：
9302689
负责人：
CARLO M CROCE
金额：
$ 91.57万
依托单位：
OHIO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-07-02 至 2019-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9302689
关键词：
Advanced Malignant Neoplasm Adverse effects Affect Age Behavior Biological Biological Markers Biology Blood Burkitt Lymphoma Cancer Etiology Cancer Patient Cells Cessation of life Chronic Lymphocytic Leukemia Cigarette Clinical Colorectal Cancer Conflict (Psychology)Data Defect Detection Development Diagnostic Disease Early Diagnosis Enrollment Epigenetic Process Etiology FHIT gene Family Frequencies Gene Expression Gene Expression Profile Gene Proteins Gene Targeting Genes Genetic Goals Hematopoietic Neoplasms Human Individual Indolent Italy KRAS2 gene Lead Lesion Link Lung Adenocarcinoma Lung Neoplasms Lymphoma Malignant - descriptor Malignant Neoplasms Malignant neoplasm of lung MicroRNAs Molecular Molecular Profiling Mutation National Cancer Institute Natural History Normal tissue morphology Observational Study Oncogenic Onset of illness Organism PTEN gene Pathogenesis Pathway interactions Patients Pattern Plasma Play Procedures Publications Publishing RNA Randomized Reporting Risk Role Sampling Scanning Smoke Smoker Solid Neoplasm Spiral Computed Tomography Sputum Structure of parenchyma of lung TP53 gene Techniques Technology Thoracic Radiography Time Tissues Tobacco smoke Translations Tumor Suppressor Genes Untranslated RNA Urine X-Ray Computed Tomography aged arm cancer diagnosis cohort cost deep sequencing early detection biomarkers epigenetic regulation follow-up high risk insight lung cancer prevention lung cancer screening lung carcinogenesis mRNA Expression mRNA Transcript Degradation malignant breast neoplasm man member mortality nano-string neoplastic novel outcome forecast prognostic protein expression public health relevance screening tool tumor tumorigenesis

项目摘要

DESCRIPTION (provided by applicant): We have shown genetic alterations and microRNA dysregulation in hematopoietic and solid tumors, including lung cancer, the major cause of cancer death in the USA. Recently, we explored microRNA expression profiles in lung tumors, normal lung tissues and plasma samples from cases with variable prognosis involved in a completed spiral CT screening trial with extensive follow up. The trial involved 1025 individuals age 50 years or older, who smoked at least a pack of cigarettes a day for 20 years or more. Profiling of plasma samples collected 1-2 years before the onset of disease, at the time of CT detection, and in disease-free smokers enrolled in the screening trial, provided microRNA expression signatures with strong predictive, diagnostic and prognostic potential (Boeri M. et al. "microRNA signatures in tissues and plasma predict development and prognosis of computed tomography detected lung cancer", Proc. Nat. Acad. Sci. USA, 108:3712-3718, March 1, 2011). These signatures were validated in an independent cohort from a second randomized spiral - CT trial. These results indicate a role of microRNAs as molecular predictors of lung cancer development and aggressiveness and have important clinical implications for lung cancer management. We propose to validate these signatures by using NanoString technology and deep sequencing of microRNAs by taking advantage of large cohorts of patients at NYU (Dr. H. Pass) and at the National Cancer Institute of Italy, Milan, (Drs. U. Pastorino and G. Sozzi). In addition, we propose to investigate the dysregulation of larger non-coding RNAs in the tumors, normal tissues and plasma in the same cohorts of patients, since it could provide novel powerful biomarkers for the early detection and prevention of lung cancer.

描述（由申请人提供）：我们已经显示出造血和实体瘤在内的遗传改变和microRNA失调，包括肺癌，这是美国癌症死亡的主要原因。最近，我们探索了来自肺部肿瘤，正常肺组织和血浆样品中的microRNA表达谱，该病例的预后涉及可变的螺旋CT筛查试验，并进行了广泛的随访。该试验涉及1025名年龄50岁以上的人，他们每天至少吸烟20年或更长时间。在疾病发作前1 - 2年，在CT检测时以及在筛查试验中注册的无疾病吸烟者中收集的血浆样品的分析，提供了具有强大预测性，诊断和预后潜力的microRNA表达特征（Boeri M.等。美国，108：3712-3718，2011年3月1日）。这些特征在第二个随机螺旋 - CT试验中在独立队列中得到了验证。这些结果表明microRNA是肺癌发育和侵略性的分子预测因子，并对肺癌管理具有重要的临床意义。我们建议通过利用纽约大学（H. Pass博士）和意大利国家癌症研究所（U. U. Pastorino和G. Sozzi博士）在NYU（H. Pass）和意大利国家癌症研究所的大量患者中使用大量患者来验证这些特征。此外，我们建议研究同一患者肿瘤，正常组织和血浆中较大非编码RNA的失调，因为它可以为早期发现和预防肺癌提供新颖的强大生物标志物。

项目成果

期刊论文数量（23）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Computational design of artificial RNA molecules for gene regulation.

DOI：
10.1007/978-1-4939-2291-8_25
发表时间：
2015
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
0
作者：
Lagana, Alessandro;Veneziano, Dario;Russo, Francesco;Pulvirenti, Alfredo;Giugno, Rosalba;Croce, Carlo Maria;Ferro, Alfredo
通讯作者：
Ferro, Alfredo

Automated Coronary Artery Calcium and Quantitative Emphysema in Lung Cancer Screening: Association With Mortality, Lung Cancer Incidence, and Airflow Obstruction.

DOI：
10.1097/rti.0000000000000698
发表时间：
2023-07-01
期刊：
Journal of thoracic imaging
影响因子：
3.3
作者：
通讯作者：

Lung cancer screening with low-dose spiral computed tomography: evidence from a pooled analysis of two Italian randomized trials.

DOI：
10.1097/cej.0000000000000264
发表时间：
2017-07
期刊：
European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP)
影响因子：
0
作者：
Infante M;Sestini S;Galeone C;Marchianò A;Lutman FR;Angeli E;Calareso G;Pelosi G;Sozzi G;Silva M;Sverzellati N;Cavuto S;La Vecchia C;Santoro A;Alloisio M;Pastorino U
通讯作者：
Pastorino U

mir-660-p53-mir-486 Network: A New Key Regulatory Pathway in Lung Tumorigenesis.

DOI：
10.3390/ijms18010222
发表时间：
2017-01-23
期刊：
International journal of molecular sciences
影响因子：
5.6
作者：
Borzi C;Calzolari L;Centonze G;Milione M;Sozzi G;Fortunato O
通讯作者：
Fortunato O

Circulating microRNA signature as liquid-biopsy to monitor lung cancer in low-dose computed tomography screening.

DOI：
10.18632/oncotarget.5210
发表时间：
2015-10-20
期刊：
Oncotarget
影响因子：
0
作者：
Sestini S;Boeri M;Marchiano A;Pelosi G;Galeone C;Verri C;Suatoni P;Sverzellati N;La Vecchia C;Sozzi G;Pastorino U
通讯作者：
Pastorino U