On-Chip Multiplexed Adhesion Frequency Assay for Measuring Receptor-Ligand Interactions on Cells

用于测量细胞上受体-配体相互作用的片上多重粘附频率测定

• 批准号: 9349129
• 负责人: Yuebing Zheng
• 金额: $ 223.58万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-30 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9349129
• 关键词: Address; Adhesions; Area; Aspirate substance; Biological Assay; Biological Process; Biomedical Research; Cell Separation; Cell physiology; Cells; Cellular biology; Communication; Development; Devices; Disease; Environment; Frequencies; Ligands; Manuals; Measurement; Measures; Mediating; Microfluidics; Optics; Route; Techniques; Therapeutic; Validation; Virus Diseases; cancer immunotherapy; clinical application; device physics; high throughput screening; optical imaging; receptor

Project Summary Receptor-ligand interactions on cells mediate cell-cell and cell-environment communications in many biological processes. Adhesion frequency assay has unique capability of measuring the receptor-ligand interactions at the single-cell level. The measurement can provide important information on the quality of biological processes and for the selection of potent therapeutic cells. Current adhesion frequency assay uses micropipettes to aspirate cells for both interaction measurement and cell transfer afterwards. As a result, it is bulky, labor-intensive, manual- operative, and low-throughput. In order to maximize the potential of adhesion frequency assay in biomedical research and clinical applications, it is necessary to develop high-throughput miniature device. In view of the tremendous potential and challenges in developing high-throughput miniature adhesion frequency assay, I propose a transformative technical route – on-chip multiplexed adhesion frequency assay – that will seek to synergize optical manipulation and microfluidics. I will bring in new ideas from different areas, including optical manipulation, optical imaging, microfluidics, cellular biology, and device physics for development, validation and applications of the proposed assay. Specifically, I will (1) address multiplexed-measurement and cell-sorting challenges in the implementation of the assay, (2) characterize, validate and optimize the pre- packaged assay, and (3) assemble and package the assay into a compact device and apply it to screen cells for disease therapy. With the unprecedented capabilities of measuring the receptor- ligand interactions and sorting cells, the proposed assay will change current paradigm of cell profiling and incorporate one of the most important parameters for quantifying cell functions into routine assays. Once fully developed, the on-chip multiplexed adhesion frequency assay will fill many unmet needs in both biomedical research and clinical applications of the receptor-ligand interactions on cells.

项目摘要 细胞上的受体配体相互作用介导细胞 - 环境和细胞环境通信 许多生物过程。粘附频率测定具有测量的独特功能 接收器配体相互作用在单细胞级别。测量可以提供重要的 有关生物过程质量和选择潜在疗法的信息 细胞。当前的粘附频率测定使用微孔吸入细胞进行两种相互作用 测量和细胞转移之后。结果，它是笨重，劳动密集型，手动 - 手术和低通量。为了最大化粘附频率测定的潜力 生物医学研究和临床应用，有必要开发高通量 微型设备。 鉴于开发高通量微型的巨大潜力和挑战 粘附频率评估，我提出了一条变革性的技术途径 - 芯片多路复用 粘附频率评估 - 将试图合成光学操纵和微流体。我 将引入不同领域的新想法，包括光学操纵，光学成像， 用于开发，验证和应用的微流体，细胞生物学和设备物理学 拟议的测定法。具体而言，我将（1）解决多路复用测量和细胞分类 （2）在实施实施时面临的挑战表征，验证和优化了 包装测定法，（3）将测定和将测定包装到紧凑的设备中，然后将其应用于 用于疾病疗法的筛查细胞。具有衡量接收器的前所未有的功能 配体相互作用和分析细胞，提出的测定将改变细胞的当前范式 分析并结合将细胞函数量化的最重要参数之一 例行测定。一旦完全开发，片上的多重广告频率测定将填充 在接收器配体的生物医学研究和临床应用中，许多未满足的需求 细胞上的相互作用。

项目成果

Nanoradiator-Mediated Deterministic Opto-Thermoelectric Manipulation.

• DOI: 10.1021/acsnano.8b05824
• 发表时间: 2018-10-23
• 期刊: ACS nano
• 影响因子: 17.1
• 作者: Liu Y;Lin L;Bangalore Rajeeva B;Jarrett JW;Li X;Peng X;Kollipara P;Yao K;Akinwande D;Dunn AK;Zheng Y
• 通讯作者: Zheng Y

A mixture-density-based tandem optimization network for on-demand inverse design of thin-film high reflectors.

• DOI: 10.1515/nanoph-2021-0392
• 发表时间: 2021-11
• 期刊: Nanophotonics
• 影响因子: 7.5

Optothermophoretic Manipulation of Colloidal Particles in Nonionic Liquids.

• DOI: 10.1021/acs.jpcc.8b03828
• 发表时间: 2018-06
• 期刊: The journal of physical chemistry. C, Nanomaterials and interfaces
• 作者: Xiaolei Peng;Linhan Lin;E. H. Hill;Pranaw Kunal;S. M. Humphrey;Yuebing Zheng

Opto-Thermophoretic Manipulation and Construction of Colloidal Superstructures in Photocurable Hydrogels.

• DOI: 10.1021/acsanm.8b00766
• 发表时间: 2018-07
• 期刊: ACS applied nano materials
• 影响因子: 5.9
• 作者: Xiaolei Peng;Jingang Li;Linhan Lin;Yaoran Liu;Yuebing Zheng

Thermo-Electro-Mechanics at Individual Particles in Complex Colloidal Systems

• DOI: 10.1021/acs.jpcc.9b06425
• 发表时间: 2019-09-05
• 期刊: JOURNAL OF PHYSICAL CHEMISTRY C
• 影响因子: 3.7
• 作者: Kollipara, Pavana Siddhartha;Lin, Linhan;Zheng, Yuebing
• 通讯作者: Zheng, Yuebing