ShEEP Request for Flow Cytometer Special Order Laser Upgrade

ShEEP 要求流式细胞仪特别订购激光升级

• 批准号: 9213430
• 负责人: MARC Lee GOALSTONE
• 金额: --
• 依托单位: VA EASTERN COLORADO HEALTH CARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2016-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9213430
• 关键词: Agonist; Alzheimer' s Disease; Animals; Antibodies; Award; Brain; CREB1 gene; Cardiovascular Diseases; Cell Culture Techniques; Characteristics; Color; Data Quality; Detection; Diabetes Mellitus; Differentiation Antigens; Doctor of Philosophy; Electronics; Equipment; Face; Financial compensation; Flow Cytometry; Health; Investigation; K-Series Research Career Programs; Kynurenine; Laboratories; Lasers; Lipids; MAPK7 gene; Malignant neoplasm of lung; Mediating; Nature; Optics; PPAR gamma; Pathway interactions; Performance; Population; Prevention; Principal Investigator; Pulmonary Fibrosis; Rehabilitation therapy; Request for Applications; Research; Research Infrastructure; Research Personnel; Research Project Grants; Resolution; Sampling; Site; Stem cells; System; TNF gene; Toxoplasma; Translating; Tube; Vascular Diseases; Veterans; Viola; abstracting; cancer chemoprevention; cost; cost effective; detector; diabetic; improved; instrument; photomultiplier; restoration; suicidal behavior; vascular inflammation

Abstract In this application we request the following upgrades to our existing Becton Dickinson LSRII Flow Cytometer: 1) Installation of a Coherent G4 355nm 60mw UV laser 2) Three photomultiplier tubes installed on an Octagon optical array and optics configured: a. BUV396 (379/28) b. BUV496 (450LP;515/30) c. BUV737 (690LP; 740/35) 3) Additional photomultiplier tube added to existing violet Octagon with optics for BV785 (750LP; 780/60) 4) Additional electronics to support the new configuration 5) FACS Flow Supply System These upgrades will increase the number of total colors we can analyze to 18 and enhance the LSRII’s performance capacity. The following research projects will be supported by the upgrades system: 1) Marc Goalstone, PhD, Principal Investigator: BLR&D Merit Review Award “Diabetes: ERK5 and Vascular Inflammation” 2) Robert Keith, MD, Principal Investigator: BLR&D Merit Review Award “PPAR gamma Agonists for Lung Cancer Chemoprevention” 3) Elizabeth Redente, PhD, Principal Investigator: BLR&D Career Development Award “Mechanisms of TNF-alpha Mediated Resolution of Pulmonary Fibrosis” 4) Subbiah Pugazhenthi, PhD, Principal Investigator: BLR&D Merit Review Award “Mechanism of CREB dysregulation in Alzheimer brain” 5) Jane Reusch, MD, Principal Investigator: BLR&D Merit Review Award “Pharmacological Restoration of Diabetic Vascular Dysfunction” 6) Lisa Brenner, PhD, Co-Principal Investigator: CSR&D Merit Review Award “Toxoplasma gondi, the kynurenine pathway, and suicidal behavior in veterans” 7) Ehrin Armstrong, MD, Local Site Investigator: CSR&D Merit Review Award “Plaque Regression and Progenitor Cell Mobilization with Intensive Lipid Elimination”

抽象的 在此应用程序中，我们要求以下升级到现有的Becton Dickinson LSRII流式细胞仪： 1）安装连贯的G4 355nm 60MW紫外线激光器 2）配置了八角形光学阵列和光学器件上安装的三个光电倍增管： 一个。 BUV396（379/28） b。 BUV496（450LP; 515/30） c。 BUV737（690LP; 740/35） 3）添加到现有的紫罗兰色八角形中的其他光电倍增管（Bv785）（750LP; 780/60） 4）其他电子设备支持新配置 5）FACS流量供应系统 这些升级将增加我们可以分析的总颜色的数量，并增强LSRII 性能能力。以下研究项目将得到升级系统的支持： 1）MARC GORTSTONE，博士，首席研究员：BLR＆D值得评论奖“糖尿病：ERK5和 血管炎症” 2）罗伯特·基思（Robert Keith），医学博士，首席研究员：BLR＆D优异评论奖“ PPAR GAMMA AGONISTS的肺部 癌症化学预防” 3）Elizabeth Redente博士，首席研究员：BLR＆D职业发展奖“机制 TNF-α介导的肺纤维化的分辨率” 4）Subbiah Pugazhenthi，博士，首席研究员：BLR＆D优异评论奖“ CREB的机制 阿尔茨海默氏症大脑的失调” 5）Jane Reusch，医学博士，首席调查员：BLR＆D优异评论奖“药理学修复 糖尿病血管功能障碍” 6）丽莎·布伦纳（Lisa Brenner）博士，联合主持调查员：CSR＆D优异评论奖“ Toxoplasma Gondi， Kynurenine途径和退伍军人的自杀行为” 7）当地现场调查员医学博士Ehrin Armstrong：CSR＆D成绩评论奖“牌匾回归和 祖细胞动员，大量脂质消除”