Development of a Low-Cost Therapy for Biological Toxins for Rural India

为印度农村地区开发低成本生物毒素疗法

基本信息

批准号：
9208599
负责人：
Claire Komives
金额：
$ 10.35万
依托单位：
SAN JOSE STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-07 至 2019-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9208599
关键词：
Address Air Amino Acids Antidotes Antitoxins Antivenins Biological Response Modifier Therapy Blood Circulation C-terminal Cell Extracts Chemicals Chromatography Cleaved cell Collaborations Country Crotalus Development Devices Didelphidae Dose Environment Equipment Escherichia coli Filtration Formulation Goals Health Services Accessibility Hemorrhage Immobilization Immune In Situ Inclusion Bodies India Industry Inhalators Institutes Lead Life Membrane Metalloproteases Methods Modeling Modification Morbidity - disease rate Mus Names North American Opossum Peptide Hydrolases Peptides Plasmids Polymers Powder dose form Procedures Process Production Proteins Research Rural Saline Series Serum Snake Bites Snake Venoms Snakes System Technology Temperature Testing Texas Therapeutic Intervention Time Toxin Trypsin Ultrafiltration Universities Venoms anthrax lethal factor cost dosage experimental study farmer inhibitor/antagonist liquid chromatography mass spectrometry mortality multidisciplinary potency testing process optimization rural setting stability testing

项目摘要

Project Summary This project involves the development of a peptide formulation that could be used as a low cost therapy for hemorrhagic snake envenomation. The loss of life due to snake bite is higher in India than in any other country. Indeed, 60% of India's voters are farmers, working in fields where venomous snakes are most prevalent. The expression of a peptide that we have confirmed to neutralize hemotoxic snake venom will be carried out using a bacterial expression system. The peptide can be purified and tested for antivenom activities at the Texas A&M National Natural Toxins Research Center. The peptide will be characterized by LC/MS. Formulations of the peptide will be sought that have long term stability at room temperature and could be appropriate for delivery as a dry powder inhalable formulation or solubilized for alternative delivery method. The mutual expertise of Komives (SJSU) and Rathore (IIT Delhi) will enable the completion of this multidisciplinary project. Specifically, the expression and production of the peptide will be lead by Komives while the purification and process optimization will be lead by Rathore. Formulation will be carried out in both labs, but primarily in the lab of Komives. The project is anticipated to establish an ongoing collaboration between San Jose State University and the India Institute of Technology Delhi.

项目概要该项目涉及开发一种肽制剂，可用作低成本治疗出血性蛇毒中毒。蛇咬伤造成的生命损失在以下国家更高印度比任何其他国家都多。事实上，60% 的印度选民是在田间劳作的农民毒蛇最盛行的地方。我们拥有的肽的表达已证实可以使用细菌表达来中和血液毒性蛇毒系统。该肽可在德克萨斯 A&M 进行纯化并测试其抗蛇毒血清活性国家天然毒素研究中心。该肽将通过 LC/MS 进行表征。将寻求在室温下具有长期稳定性的肽制剂，并且可以适合作为干粉吸入制剂输送或溶解替代交付方式。 Komives (SJSU) 和 Rathore (IIT Delhi) 的共同专业知识将有助于完成这个多学科项目。具体而言，肽的表达和产生将是由 Komives 领导，而纯化和工艺优化将由 Rathore 领导。配方将在两个实验室进行，但主要在 Komives 实验室进行。该项目是预计圣何塞州立大学和印度理工学院德里。