CSHL X-Ray Methods in Structural Biology

结构生物学中的 CSHL X 射线方法

基本信息

批准号：
8539053
负责人：
TERRI I. GRODZICKER
金额：
$ 8.15万
依托单位：
COLD SPRING HARBOR LABORATORY
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-09-01 至 2017-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8539053
关键词：
Area Biochemistry Biological Cancer Biology Complex Crystallization Crystallography Data Data Collection Deposition Development Discipline Disease Doctor of Medicine Doctor of Philosophy Drug Design Educational Curriculum Educational process of instructing Ensure Faculty Freezing Funding Industry Knowledge Laboratories Learning Left Light Malignant Neoplasms Maps Medical Students Methods Molecular New York Nucleic Acids Optics Participant Pharmaceutical Preparations Postdoctoral Fellow Process Proteins Research Research Personnel Roentgen Rays Running Scientist Series Solvents Source Structure Students Synchrotrons Techniques Training Tumor Suppressor Genes United States Universities Update Validation Virus Work X ray diffraction analysis X-Ray Crystallography X-Ray Diffraction anticancer research arm base biological research data reduction design electron density experience falls graduate student instructor lecturer lectures macromolecule medical schools method development programs protein function protein structure protein structure function research study structural biology theories three dimensional structure translational approach tumor

项目摘要

DESCRIPTION (provided by applicant): Cold Spring Harbor Laboratory proposes to continue the course on X-Ray Methods in Structural Biology to be held in the fall of 2012-2016. Crystallography and X-ray diffraction yield a wealth of structural information unobtainable through other methods. This intensive 16-day laboratory/computational course focuses on the major X-ray crystallographic techniques used to determine the three-dimensional structures of macromolecules. The course provides the opportunity for research workers in different biological disciplines to become grounded in the techniques and principles of macromolecular crystallography. It is designed for scientists with a working knowledge of protein structure and function, but who are new to macromolecular crystallography. Topics to be covered include basic diffraction theory, crystallization (proteins, nucleic acids, viruses and complexes), crystal characterization, X-ray sources and optics, synchrotrons, crystal freezing, data collection, X-ray data reduction, multiple isomorphous replacement, multiwavelength/single wavelength anomalous diffraction, molecular replacement, solvent flattening, non-crystallographic symmetry averaging, electron density map interpretation, molecular graphics, structure refinement, structure validation, coordinate deposition, structure presentation along with other relevant topics. The lecturers are chosen on the basis of their contributions to, and knowledge of, the areas covered in the course. In addition, several of the lecturers present their current research to illustrate the principles that are taught in the course. Participants learn through extensive hands-on experiments where they crystallize and determine protein structures, along with lectures and informal discussions on the theory behind the techniques from world leaders in methods development. The course day starts in the morning and runs throughout the day until late at night. There is also a trip to the National Synchrotron Light Source facility at Brookhaven National Laboratory to collect multiwavelength X-ray diffraction data. Applications are open to a wide range of students including advanced graduate students, medical students, post-doctoral fellows, faculty at universities and medical schools, as well as Ph.D. and M.D. scientists from industry. The course is designed to allow students to learn techniques that can be immediately incorporated into their own research. This enables the training of new researchers who can not only solve structures relevant to their field of biological endeavor but also contribute to translational approaches including rational drug design.

描述（由申请人提供）：冷泉港实验室建议继续在 2012-2016 年秋季举办结构生物学 X 射线方法课程。晶体学和 X 射线衍射可提供其他方法无法获得的大量结构信息。这个为期 16 天的强化实验室/计算课程重点关注用于确定大分子三维结构的主要 X 射线晶体学技术。该课程为不同生物学科的研究人员提供了了解大分子晶体学技术和原理的机会。它是为具有蛋白质结构和功能的工作知识但对大分子晶体学不熟悉的科学家而设计的。涵盖的主题包括基本衍射理论、结晶（蛋白质、核酸、病毒和复合物）、晶体表征、X射线源和光学、同步加速器、晶体冷冻、数据收集、X射线数据缩减、多重同晶替换、多波长/单波长异常衍射、分子替换、溶剂平坦化、非晶体对称性平均、电子密度图解释、分子图形、结构细化、结构验证、坐标沉积、结构呈现以及其他相关主题。讲师的选择是基于他们对课程所涵盖领域的贡献和知识。此外，几位讲师还介绍了他们当前的研究，以说明课程中教授的原理。参与者通过大量的实践实验来学习，在这些实验中，他们结晶并确定蛋白质结构，以及来自方法开发领域的世界领导者关于技术背后的理论的讲座和非正式讨论。课程日从早上开始，全天持续到深夜。还可以参观位于布鲁克海文的国家同步加速器光源设施国家实验室收集多波长X射线衍射数据。申请向广泛的学生开放，包括高级研究生、医学生、博士后研究员、大学和医学院的教师以及博士生。以及来自工业界的医学博士科学家。该课程旨在让学生学习可以立即融入自己研究的技术。这使得能够培训新的研究人员，他们不仅可以解决与其生物学领域相关的结构，还可以为包括合理药物设计在内的转化方法做出贡献。