TRANSGENIC MOUSE AND EMBRYONIC STEM CELL FACILITY

转基因小鼠和胚胎干细胞设施

• 批准号: 8486642
• 负责人: MICHELLE M LE BEAU
• 金额: $ 13.52万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-04-01 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8486642
• 关键词: Brain; Breeding; Cancer Biology; Cancer Center Support Grant; Cell Culture Techniques; Cell model; Cells; Chicago; Colon; Comprehensive Cancer Center; DNA; ES Cell Line; Embryo; Equipment; Female; Gene Targeting; Generations; Genes; Genetic Engineering; Genome; Human Resources; Individual; Instruction; Laboratory mice; Maintenance; Malignant Neoplasms; Microinjections; Modeling; Molecular; Molecular Genetics; Mus; Pregnancy; Preparation; Production; Prostate; Research; Research Design; Research Personnel; Resource Sharing; Services; Skin; Southern Blotting; Staging; Technology; Time; Transgenic Animals; Transgenic Mice; Transgenic Organisms; Universities; animal resource; cancer therapy; cost; design and construction; embryonic stem cell; experience; human disease; malignant breast neoplasm; member; mouse model; pregnant; research facility; success; tool; vector; web page; web site

The University of Chicago Comprehensive Cancer Center (UCCCC) Transgenic Mouse/Embryonic Stem Cell Facility provides comprehensive genetic engineering services to alter the genome of the laboratory mouse. The UCCCC has many invesfigators who use mouse models as their primary tool of analysis, as well as a multitude of addifional investigators who require the occasional mouse model in their studies of the causes and treatment of cancer. The generation and maintenance of transgenic animals through the microinjection of single-celled mouse embryos, and the generation of genetically modified mice through the use of ES cells require specialized technical personnel. Furthermore, such efforts necessitate the acquisition of an array of devoted equipment. Thus, the availability of a shared resource greatly reduces research costs for the individual investigators of the UCCCC. The existence of this Facility also greatly increases the accessibility of genetic engineering technology to investigators with limited related experience. The UCCCC Transgenic Mouse/Embryonic Stem Cell Facility was established in 1991, and has been tremendously productive and successful; the range of services provided has expanded considerably since it's founding. Services provided by the Transgenic Mouse/Embryonic Stem Cell Facility include: 1) transgenic mouse production from founder through F1 Stage; 2) ES cell technology mouse production; 3) ES cell gene targeting and culturing; 4) embryo rederivation; 5) mouse embryonic feeder (MEF) cell production; 6) Timed pregnancies of various strains and lines of mice; 7); various breeding services and GEM model line maintenance; 8) DNA preparation from ES cell lines; and 9) design and construction of transgenic or ES cell targeting vectors. In addition to the technical services, the Facility also offers assistance with the design of studies that require mouse molecular genetics, as well as advice and instruction on mouse handling and breeding. In providing these comprehensive services, the UCCCC Transgenic Mouse/Embryonic Stem Cell Facility has generated mouse models that have led to advances in our understanding of cancer, including cancers of the breast, skin, colon, brain and prostate.

芝加哥大学综合癌症中心 (UCCCC) 转基因小鼠/胚胎干细胞设施提供全面的基因工程服务，以改变实验室小鼠的基因组。 UCCCC 有许多研究人员使用小鼠模型作为主要分析工具，还有许多其他研究人员在癌症成因和治疗研究中偶尔需要使用小鼠模型。通过显微注射单细胞小鼠胚胎产生和维持转基因动物，以及通过使用ES细胞产生转基因小鼠，都需要专门的技术人员。此外，此类努力需要采购一系列专用设备。因此，共享资源的可用性大大降低了 UCCCC 个体研究人员的研究成本。该设施的存在也大大增加了相关经验有限的研究人员获得基因工程技术的机会。 UCCCC 转基因小鼠/胚胎干细胞设施成立于 1991 年，取得了巨大的成效和成功；自成立以来，所提供的服务范围已大大扩展。转基因小鼠/胚胎干细胞设施提供的服务包括： 1) 从创始人到F1阶段的转基因小鼠生产； 2）ES细胞技术小鼠 生产; 3）ES细胞基因打靶及培养； 4）胚胎再衍生； 5)小鼠胚胎饲养层(MEF)细胞的制作； 6) 各品系、系小鼠的定时妊娠； 7);各种繁育服务及GEM模型系维护； 8) ES细胞系DNA的制备； 9) 转基因或ES细胞靶向载体的设计和构建。除了技术服务外，该设施还提供需要小鼠分子遗传学的研究设计方面的帮助，以及有关小鼠处理和育种的建议和指导。在提供这些全面的服务时，UCCCC 转基因小鼠/胚胎干细胞设施已生成具有以下特征的小鼠模型： 促进了我们对癌症的理解，包括乳腺癌、皮肤癌、结肠癌、脑癌和前列腺癌。