Study of Accessory Lacrimal Gland and Precursor Cell Biology

副泪腺及前体细胞生物学研究

• 批准号: 9044784
• 负责人: Vinay Aakalu
• 金额: $ 23.64万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-01 至 2019-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9044784
• 关键词: ABCG2 gene; Address; Affect; Age; Anatomy; Atrophic; Basic Science; Biology; Blindness; Cell Separation; Cell Survival; Cells; Cellular biology; Characteristics; Chicago; Clinical; Clinical Investigator Award; Development; Disease; Ethics; Eye; Eye diseases; Film; Fluorescence; Gender; Gland; Goals; Growth; Harvest; Health; Histologic; Hormonal; Human; Illinois; Immunohistochemistry; Investigation; K-Series Research Career Programs; Knowledge; Lacrimal gland structure; Lead; Location; Lubrication; Maintenance; Maps; Mentors; Microscopic; Molecular; Mus; Ocular orbit; Operative Surgical Procedures; Patients; Pattern; Performance; Persons; Population; Production; Productivity; Property; Quality of life; Race; Regenerative Medicine; Research; Research Personnel; Scientist; Specimen; Stem cells; Surface; Techniques; Thinking; Tissue Microarray; Tissues; Training; Translational Research; Transplantation; Treatment Cost; Universities; Work; age effect; aqueous; base; career; chronic pain; clinical practice; corneal scar; experience; eye dryness; lacrimal; ocular surface; older patient; precursor cell; programs; research and development; skills; stem cell biology; symposium; translational clinical trial; vision science

DESCRIPTION (provided by applicant): This is a proposal for a K08 Mentored Clinical Scientist Career Development Award to train Dr. Vinay Kumar Aakalu. The primary goal of this application is to serve as a basis for training Dr. Aakalu in the critical skills necessary to deveop into an independent scientific investigator focused on lacrimal cell and precursor cell biology. The specific aims of the proposed educational program are the performance of 1) graduate level cell, molecular, stem cell biology and basic translational/clinical trial course work at the University of Illinois at Chicago 2) basic scientific investigations in accessory lacrimal cell and stem cell biology under the guidance of an experienced four person mentoring committee 3) seminar and conference participation at the University of Illinois and outside the University of Illinois. The mentoring committee is made up of experts in stem cell, ocular, molecular cell biology and translational research. Each mentor is well experienced in training clinician- scientists. The mentoring committee will be focused on guiding Dr. Aakalu in the development of research skills, analytical thinking and the ethical performance of basic and translational research. The overarching research goals of the proposal are to develop a better understanding of accessory lacrimal cell biology and to identify and isolate precursor cells from this tissue. Th specific aims of the project are to: 1. Develop a better understanding of ALG distribution, growth requirements, and changes with patient characteristics. 1a. A three dimensional map of the cadaveric human orbit will be constructed to determine the location of ALG tissue throughout the ocular surface as a basis for rational harvesting decisions. 1b. Characterize ALG tissue in human surgical specimens and relate the robustness or histopathologic changes in these tissues to relevant patient characteristics (age, gender, race, hormonal status). 1c. Develop ALG cultures from human surgical specimens to better understand requirements for cell survival and expansion. 2. Establish the presence of precursor cells in ALG tissue and isolate and characterize these precursor cells. 2a. Identify precursor marker positive ALG cells and determine a signature marker pattern using human specimens and immunohistochemistry on tissue microarrays. 2b. Isolate precursor candidate cells utilizing fluorescence cell sorting and determine if changes in precursor candidate cell populations are noted with different patient characteristics. 2c. Determine if precursor candidate cells isolated have stem cell properties. 2d. Establish cultures from precursor candidates and determine if these cultures can produce functional ALG cells.

描述（由申请人提供）：这是K08指导的临床科学家职业发展奖，旨在培训Vinay Kumar Aakalu博士。该应用的主要目的是作为培训Aakalu博士的基础，以将其脱离到专注于泪腺细胞和前体细胞生物学的独立科学研究者所需的关键技能。拟议的教育计划的具体目的是表现1）研究生水平细胞，分子，干细胞生物学以及伊利诺伊大学芝加哥大学的基本转化/临床试验课程工作2）附属乳细胞和附属细胞的基础科学调查 在经验丰富的四人指导委员会的指导下，干细胞生物学3）在伊利诺伊大学和伊利诺伊大学以外的会议和会议参与。指导委员会由干细胞，眼科，分子细胞生物学和转化研究专家组成。每个导师在培训临床医生科学家方面都有丰富的经验。指导委员会将重点介绍指导Aakalu博士的研究技能，分析思维以及基本和转化研究的道德表现。该提案的总体研究目标是更好地理解对辅助泪细胞生物学的理解，并鉴定和分离前体细胞与该组织。该项目的具体目的是：1。对ALG分布，增长要求以及随患者特征的变化有更好的了解。 1a。将构建尸体人轨道的三维图，以确定整个眼表中的ALG组织的位置，作为合理收获决策的基础。 1B。表征人类手术标本中的ALG组织，并将这些组织的鲁棒性或组织病理学变化与相关的患者特征（年龄，性别，种族，荷尔蒙状态）联系起来。 1C。从人类手术标本中开发ALG培养物，以更好地了解细胞存活和膨胀的要求。 2。在ALG组织中建立前体细胞的存在并分离并表征这些前体细胞。 2a。识别前体标记阳性ALG细胞，并使用人类标本和组织微阵列上的免疫组织化学确定签名标记模式。 2b。利用荧光细胞分选的分离株前体候选细胞，并确定前体候选细胞群体的变化是否具有不同的患者特征。 2C。确定前体候选细胞是否分离出具有干细胞特性。 2d。 从前体候选物中建立培养物，并确定这些培养物是否可以产生功能性ALG细胞。