Confocal/Multiphoton Microscope Upgrade

共焦/多光子显微镜升级

• 批准号: 8826419
• 负责人: John J Lemasters
• 金额: $ 57.05万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-02-15 至 2016-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8826419
• 关键词: 8 year old; Cancer Center; Cell Death; Computer software; Cost Savings; Detection; Environment; Equipment; Goals; Image; Imaging Techniques; Immersion Investigative Technique; Individual; Injury; Lasers; Medical; Microscope; Microscopy; Natural regeneration; Optics; Performance; Research Personnel; Scanning; South Carolina; System; Training; Universities; Water; Work; base; cellular imaging; detector; in vivo imaging; molecular imaging; optical imaging; vibration

 DESCRIPTION (provided by applicant): The Cell and Molecular Imaging (CMI) Facility is a shared component of the Center for Cell Death, Injury and Regeneration (CCDIR) and the NCI-designated Hollings Cancer Center (HCC) whose purpose is to provide CCDIR/HCC investigators and other researchers at the Medical University of South Carolina (MUSC) with equipment and expertise for laser scanning confocal/multiphoton microscopy and related high-end optical imaging techniques. The only access of our investigators to combined confocal/mulitphoton microscopy is an 8+ year old Zeiss 510 NLO META microscope that is deficient in important functionalities that are essential for high performance confocal/multiphoton and intravital (in vivo) imaging, including spectral detection, long working distance water immersion optics and maximally sensitive descanned and nondescanned detection. Accordingly, the overall goal of this application is to upgrade the Zeiss 510 NLO microscope to an optimally configured and fully functional confocal/multiphoton microscope system to be made available to CCDIR, HCC and MUSC investigators. We propose to upgrade this system to a Zeiss 780 confocal/multiphoton inverted laser scanning microscope with maximally sensitive 3-channel non-descanned GaAsP detectors, a GaAsP-based 34-channel spectral array detector, 6 visible laser lines for confocal imaging, high performance long working distance intravital water-immersion optics and Zen software. Cost savings will be achieved by using microscope objectives, multiphoton laser, vibration isolation table and environmental chamber from the old microscope, whose remaining components will be traded in. The upgraded microscope system will be placed in a multiuser environment that provides technical support, user training and applications expertise in support of individual user projects.

 描述（由申请人提供）：细胞和分子成像 (CMI) 设施是细胞死亡、损伤和再生中心 (CCDIR) 和 NCI 指定的霍林斯癌症中心 (HCC) 的共享组成部分，其目的是提供 CCDIR /南卡罗来纳医科大学 (MUSC) 的 HCC 研究人员和其他研究人员拥有激光扫描共焦/多光子显微镜和相关高端光学成像的设备和专业知识我们的研究人员使用共焦/多光子组合显微镜的唯一途径是使用了 8 年以上的 Zeiss 510 NLO META 显微镜，该显微镜缺乏高性能共焦/多光子所必需的重要功能。 和活体（体内）成像，包括光谱检测、长工作距离水浸光学器件以及最灵敏的退扫描和非退扫描检测。因此，该应用的总体目标是将 Zeiss 510 NLO 显微镜升级为优化配置且功能齐全的共焦。 /多光子显微镜系统可供 CCDIR、HCC 和 MUSC 研究人员使用。我们建议将该系统升级为 Zeiss 780 共焦/多光子。倒置激光扫描显微镜，配备最灵敏的 3 通道非退扫描 GaAsP 探测器、基于 GaAsP 的 34 通道光谱阵列探测器、用于共焦成像的 6 条可见激光线、高性能长工作距离活体水浸光学器件和 Zen 软件。通过使用旧显微镜的显微镜物镜、多光子激光器、隔振台和环境室，可以节省成本，旧显微镜的其余组件将被以旧换新。升级后的显微镜系统将放置在多用户环境中，提供支持个人用户项目的技术支持、用户培训和应用专业知识。