Evaluating the effect of splicing mutations on isoform networks in autism

评估剪接突变对自闭症亚型网络的影响

基本信息

批准号：
9101077
负责人：
LILIA M IAKOUCHEVA
金额：
$ 42.04万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-05-01 至 2021-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9101077
关键词：
16p11.2 Animal Model Attention Autistic Disorder Binding Biology Brain CRISPR/Cas technology Cells Copy Number Polymorphism Data Data Set Databases Development Disease Exhibits Future Gene Expression Genes Genotype-Tissue Expression Project Goals Human Information Networks Maps Mental disorders Methods Modeling Molecular Mutate Mutation Neurons Pathway interactions Patients Play Post-Translational Protein Processing Process Property Protein Isoforms Proteins Proteomics RNA Splicing Reading Reporting Risk Role Site Specificity Splice-Site Mutation Technology Testing Therapeutic Intervention Tissues Transcript Work Yang autism spectrum disorder base brain size disease-causing mutation exome follow-up genome sequencing human disease induced pluripotent stem cell insight interest molecular phenotype mouse model mutant nerve stem cell protein expression protein protein interaction public health relevance transcriptome sequencing transcriptomics whole genome

项目摘要

DESCRIPTION (provided by applicant): A large number of mutations contributing to psychiatric disorders have been identified. However, the mechanisms by which these mutations cause these diseases are still unknown. Computational approaches that integrate large and heterogeneous datasets will play crucial role in predicting molecular mechanisms behind psychiatric disease mutations. In our recent study (Lin et al, Neuron, 2015) we demonstrated how spatio-temporal protein interaction networks could help to identify a pathway that is likely involved in regulating brain size in the 16p11.2 copy number variant deletion and duplication carriers. Here, we propose to use similar approaches to construct the isoform-level co-expression and protein interaction networks for predicting functional impact of the de novo splice site mutations from the patients with autism spectrum disorder (ASD). More than 80 splice site de novo mutations are currently identified in the ASD patients, but not a single disease mechanism is established for any of these mutations. We hypothesize that isoform-level networks will provide us with a more detailed and realistic picture of the processes that are disrupted by the ASD mutations in the brain. To test this hypothesis, we propose an integrative approach that combines network biology, CRISPR/Cas technology, transcriptomic and proteomic methods to predict and validate the impact of splice site mutations on cellular and molecular pathways in the human (iPSCs) and animal models of ASD. The ultimate goal of this project is to predict and validate specific pathways that are impacted by the de novo ASD splice site mutations. We will achieve this goal through the following specific aims: (1) Build and analyze isoform-level networks of brain co-expressed and physically interacting proteins; (2) Map de novo ASD mutations onto isoform-level networks to predict their functional impact; (3) Validate the disrupted networks and pathways using CRISPR/Cas technology in neuronal and animal models. The proposed study will discover and characterize cellular and molecular processes that are disrupted by the de novo splice site ASD mutations. The pathways identified in this study could represent important new targets for future therapeutic intervention.

描述（由适用提供）：已经确定了大量导致精神疾病的突变。但是，这些突变引起这些疾病的机制仍然未知。整合大型和异质数据集的计算方法将在预测精神病突变背后的分子机制中起关键作用。在我们最近的研究（Lin等，Neuron，2015）中，我们证明了时空蛋白相互作用网络如何有助于确定可能参与调节16p11.2拷贝数变化删除和重复载体中大脑大小的途径。在这里，我们建议使用类似的方法来构建同工型级共表达和蛋白质相互作用网络，以预测自闭症谱系障碍患者（ASD）患者的从头剪接部位突变的功能影响。我们假设，超过80个剪接站点从头突变，同工级网络将为我们提供更详细，更现实的图景，这些过程被大脑中的ASD突变所破坏的过程。为了检验这一假设，我们提出了一种结合网络生物学，CRISPR/CAS技术，转录组和蛋白质组学方法的综合方法，以预测和验证剪接位点突变对人（IPSC）（IPSC）和ASD动物模型的细胞和分子途径的影响。该项目的最终目标是预测和验证受ASD剪接位点突变影响的特定途径。我们将通过以下特定目的实现此目标：（1）构建和分析大脑共表达和物理相互作用蛋白的同工型水平网络；（2）将从头ASD突变映射到同工型级网络上，以预测其功能影响；（3）在神经元和动物模型中使用CRISPR/CAS技术验证残疾网络和途径。拟议的研究将发现并表征被从头剪接部位ASD突变破坏的细胞和分子过程。这项研究中确定的途径可以代表未来治疗干预的重要新目标。