Quorum Sensing Regulation of EHEC Virulence Genes

肠出血性大肠杆菌毒力基因的群体感应调控

• 批准号: 10384063
• 负责人: VANESSA SPERANDIO
• 金额: $ 62.7万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-01-01 至 2027-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10384063
• 关键词: 2 year old; 2-arachidonylglycerol; Adrenergic Agents; Animals; Antibiotics; Applications Grants; Attenuated; Beta-glucuronidase; Biochemical; Cannabidiol; Cannabinoids; Carbon; Child; Citrobacter rodentium; Colitis; Disease; Down-Regulation; Endocannabinoids; Enterocytes; Enzymes; Epinephrine; Epithelial Cells; Equilibrium; Escherichia coli; Escherichia coli EHEC; FDA approved; Gene Expression; Gene Expression Regulation; Genes; Genetic; Glucuronates; Goals; Grant; Hormones; In Vitro; Infection; Instruction; Intestines; Isomerase; Knock-out; Knowledge; Large Intestine; Lesion; Liposomes; Maps; Mediating; Membrane; Mus; Neurotransmitters; Norepinephrine; Oxidopamine; Pathogenesis; Pathogenicity Island; Pathway interactions; Plants; Principal Investigator; Process; Production; Progress Reports; Quantitative Reverse Transcriptase PCR; Regulation; Regulon; Research; Role; Signal Transduction; Source; Stress; Sugar Acids; System; Translating; United States National Institutes of Health; Virulence; Western Blotting; endogenous cannabinoid system; experimental study; microbiota; mouse model; mutant; novel therapeutic intervention; pathogen; pharmacologic; prevent; programs; protein expression; protein-histidine kinase; quorum sensing; receptor; reconstitution; response; transcriptomics; uronate; virulence gene

See continuation page 24 for project summary. Per NIH instructions, submit a one-page abstract of the research plan for the entire extension period (3-5 years) RELEVANCE (See instructions): The goal of this project is to investigate how different host neurotransmitters impact the balance among microbiota-pathogen-host interactions. We will assess how the stress hormones epinephrine and norepinephrine have their impact on enterohemorrhagic E. coli (EHEC) virulence gene regulation counteracted by an endocannabi noi d. We will also investigate how the microbiota alters the availability of the active form of these neurotransmitters in the gut.

项目摘要请参见续页 24。根据 NIH 的指示，提交一页摘要 整个延长期（3-5年）的研究计划 相关性（参见说明）： 该项目的目标是研究不同宿主神经递质如何影响之间的平衡 微生物群-病原体-宿主相互作用。我们将评估应激激素肾上腺素和 去甲肾上腺素对肠出血性大肠杆菌 (EHEC) 毒力基因调控有影响 被内源性大麻素所抵消 d.我们还将研究微生物群如何改变可用性 这些神经递质在肠道中的活性形式。