Genome wide profiling of histone variants in tumors

肿瘤中组蛋白变异的全基因组分析

• 批准号: 9343839
• 负责人: Yamini Praful Dalal
• 金额: $ 39.58万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9343839
• 关键词: Address; Automobile Driving; Centromere; Chromatin Structure; Chromosomal Instability; Chromosome Fragile Sites; Epigenetic Process; Failure; Future; Genes; Genome; Goals; Histones; Human; Lead; Location; Maps; Methods; Modification; Outcome; Process; Publishing; Specificity; Untranslated RNA; Variant; Work; cancer cell; centromere protein A; colon cancer cell line; follow-up; genome-wide; new technology; response; tumor; tumor progression; tumorigenic

We have mapped unique histone variant genome wide in colorectal cancer cell lines and found that that they cluster at a subset of epigenetically distinctive regions. We have mapped these regions further using cytological methods and discovered a new centromere-like region in a fragile chromosome site encompassing the myc locus. Future goals involve mapping histone variants in tumors in order to track progression of aberrant chromatin structures as a consequence of the tumorigenic process, and discovering remodelers and processes involved in the mis-localization. We are also purifying large quantities of histone variants and examining their modification status in response to tumor progression.

我们在结直肠癌细胞系中绘制了独特的组蛋白变异基因组图谱，发现它们聚集在表观遗传学独特区域的子集上。我们使用细胞学方法进一步绘制了这些区域的图谱，并在包含 myc 基因座的脆弱染色体位点中发现了一个新的着丝粒样区域。未来的目标包括绘制肿瘤中的组蛋白变异，以追踪致瘤过程导致的异常染色质结构的进展，并发现参与错误定位的重塑因子和过程。我们还纯化了大量的组蛋白变体并检查它们响应肿瘤进展的修饰状态。