Biochemistry

生物化学

• 批准号: 9123603
• 负责人: WOLFGANG BAEHR
• 金额: $ 17.21万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9123603
• 关键词: Area; Award; Basic Science; Biochemical; Biochemistry; Biosensor; Carotenoids; Cells; Collaborations; DNA; Detection; Disease; Electroporation; Evolution; Faculty; Fatty Acids; Gel; Genes; Glaucoma; Grant; High Pressure Liquid Chromatography; Image; Immunoblotting; Individual; Laboratories; Ligands; Lipofuscin; Mass Fragmentography; Molecular Biology; Neonatal; Proteins; Proteomics; Radioactivity; Research; Research Personnel; Resources; Retinal Degeneration; Retinoids; Roentgen Rays; Services; Surface; Surface Plasmon Resonance; Technical Expertise; Training; Translational Research; Ultracentrifugation; Universities; Utah; Vision research; Western Blotting; base; data archive; developmental disease; in vivo; metabolomics resource; screening; small molecule; technology training; working group

Project Summary The OVERALL aims of this Vision Research Core (VRC) are to provide: · access to resources outside the scope of individual R01 awards · access to technical expertise outside the scope a single laboratory · staff training to remove barriers to efficient translational research and collaboration The research areas supported by the VRC span the analysis and treatments of retinal degenerations, developmental disorders, glaucoma and other disorders, as well as and a range of cutting-edge basic science initiatives. We have implemented four resource modules that continue the natural evolution of how this research group works together, serving 15 investigators holding 21 NEI R01 awards. The Biochemistry Module evolved from a gene sequencing service towards a true proteomics (ultracen- trifugation, gel quantitation) and metabolomics resource (specialized GC-MS, HPLC-MS, surface plas- mon resonance) more accurately reflecting our current research strengths. Specifically the Biochemistry Module provides: · Ultracentrifugation based on a workhorse Beckman XL-90 · IR imaging and quantitatiion of immunoblots and hybridization blots · High-capacity Western blotting and ISH screening; · X-ray film developing for ECL and radioactivity detection · Neonatal in vivo electroporation of DNA constructs into cells · HPLC-GC/MS of small molecules (retinoids, carotenoids, lipofuscin components, fatty acids) · Protein/ligand interaction quantitation with SPR biosensor technologies · training and comprehensive data archiving.

项目摘要 该视觉研究核心（VRC）的总体目的是提供： ·访问个人R01奖项范围之外的资源 ·范围外的技术专业知识 ·员工培训以消除效率转化研究和协作的障碍 VRC支持的研究领域跨越了视网膜变性的分析和处理， 发育障碍，青光眼和其他疾病以及一系列尖端的基本 科学计划。 该研究小组如何共同努力，为15名持有21个NEI R01奖项的调查人员提供服务。 生物化学模块从基因测序服务演变为真正的蛋白质组学（超级蛋白质组学 三分化，凝胶量）和代谢组学资源（专业的GC-MS，HPLC-MS，表面质量 共振）更准确地反映了我们当前的研究优势。 特别是生物化学模块提供： ·基于主力贝克曼XL-90的超速离心 ·免疫印迹和杂交印迹的红外成像和定量 ·高容量的蛋白质印迹和ISH筛选； ·用于放射性检测的X射线纤维开发 ·DNA构建体的新生儿在体内电穿孔到细胞 ·小分子的HPLC-GC/MS（类类类药物，类胡萝卜素，脂肪素成分，脂肪酸） ·与SPR生物传感器技术的蛋白质/配体相互作用定量 ·培训和全面数据归档。