Project 1 - Salk Institute for Biological Studies NINDS Center Core Grant

项目 1 - 索尔克生物研究所 NINDS 中心核心资助

• 批准号: 8867295
• 负责人: KUO-FEN LEE
• 金额: $ 6.81万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-06-01 至 2017-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8867295
• 关键词: Biological; Center Core Grants; Consult; Development; Disease; ES Cell Line; Funding; Generations; Genetic; Genetically Engineered Mouse; Genome; Goals; In Vitro; Individual; Institutes; Lead; Modification; Mus; National Institute of Neurological Disorders and Stroke; Neurophysiology - biologic function; Neurosciences; Production; Reagent; Research Personnel; Research Project Grants; Services; Structure; Technology; Testing; Time; Transgenic Mice; animal model development; cost; embryonic stem cell; in vivo; knockout gene; meetings; mouse genome; mouse model; mutant; tool; vector

Individual NINDS grantees have strived to employ and develop cutting-edge genetic tools to manipulate the mouse genome to test specific hypotheses proposed in their NINDS-funded research projects. Producing many of these lines has required multiple steps in generating mouse ES cells and these lines are often laborious or difficult to obtain. The cost and time for generating such ES lines is a major bottleneck for successful animal model development as it prohibits many NINDS funded investigators from generating genetically-engineering mice, even for labs that are versed in the required advanced and very specific technologies. A central genome modification core will provide services to break the barriers to generate genetically modified mouse lines by supporting the production of designer ES-cell lines. A centralized GM Core will increase efficiency and lower the cost of generating such lines. In addition, a central service will provide opportunities for synergistic development of lines that will be of value across all user groups. The Institute has made a commitment to provide space for establishment of the GM core that would lead the way to meet the evolving needs of Neuroscience Center investigators. The major goal of the GM Core is to provide services for manipulation ofthe mouse genome in ES cells that are essential for studies ofthe mechanisms of neural function, structure and development as well as the generation of both in vitro and in vivo mouse models of diseases.

各个 NINDS 受资助者都努力雇用和培养尖端技术 操纵小鼠基因组的遗传工具，以测试其 NINDS 资助的项目中提出的特定假设 研究项目。生产许多此类细胞系需要多个步骤来生成小鼠 ES 细胞，并且 这些线通常很费力或很难获得。生成此类 ES 线的成本和时间是一个主要因素 成功动物模型开发的瓶颈，因为它阻止许多 NINDS 资助的研究人员 产生基因工程小鼠，即使是那些精通所需的先进和非常先进的实验室 具体技术。中央基因组修饰核心将提供服务来打破障碍 通过支持设计 ES 细胞系的生产来产生转基因小鼠系。一个集中式的 GM Core 将提高效率并降低生产此类线路的成本。此外，中央服务将 为对所有用户群体都有价值的产品线的协同开发提供机会。这 研究所已承诺为建立通用汽车核心提供空间，这将引领 满足神经科学中心研究人员不断变化的需求。 GM Core 的主要目标是提供 在 ES 细胞中操作小鼠基因组的服务对于研究小鼠胚胎干细胞的机制至关重要 神经功能、结构和发育以及体外和体内小鼠模型的生成 的疾病。