Rapid Biomonitoring of Volatile Toxins in Human Samples (BIO-VOTO)

人体样本中挥发性毒素的快速生物监测 (BIO-VOTO)

• 批准号: 8913660
• 负责人: Lormen Lue
• 金额: $ 22.5万
• 依托单位: CHROMOLOGIC, LLC
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-01 至 2017-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8913660
• 关键词: Acetone; Air; Biochemical; Biological; Biological Monitoring; Blood; Blood Plasma Volume; Blood specimen; Breathing; Centers for Disease Control and Prevention (U.S.); Cessation of life; Chemicals; Computer software; Detection; Device or Instrument Development; Devices; Diagnostic; Doctor of Philosophy; Double-Blind Method; Dust; Ecology; Environment; Environmental Health; Equipment; Evaluation; Exposure to; Food; Goals; Gold; Guidelines; Health; Human; Human Milk; In Vitro; Ingestion; Isotopes; Laboratories; Lead; Logic; Measurement; Measures; Meconium; Methods; Monitor; National Health and Nutrition Examination Survey; Nutritional; Optical Instrument; Optics; Performance; Persons; Phase; Plasma; Population; Preparation; Public Health; Publishing; Research Personnel; Resources; Risk; Sampling; Sensitivity and Specificity; Serum; Soil; Solid; Source; Specificity; Symptoms; System; Technology; Testing; Touch sensation; Toxic Environmental Substances; Toxin; Urine; Validation; Water; Whole Blood; Work; computerized data processing; cost; cost effective; design; environmental chemical; epidemiology study; flexibility; innovation; instrument; instrumentation; irritation; novel; programs; prototype; public health relevance; rapid technique; research facility; respiratory; sample collection; screening; standard measure; success; volatile organic compound

 DESCRIPTION (provided by applicant): Biomonitoring measures chemicals and their metabolites in a person's biological samples, such as blood or urine. It indicates the amount of exposure to chemicals by humans from breathing, touching and ingestion combined from sources including but not limited to air, soil, water, dust, and food. Through the National Biomonitoring Program (NBP) and The National Health and Nutrition Examination Survey, the CDC has established gold standard methods to measure over 450 chemicals and nutritional markers in blood, serum, urine, breast milk and meconium. However, a portable and versatile system is needed to analyze multiple analytes from similar range of samples at the point of sample collection to significantly increase detection accuracy. Additionally, alternative lower cos method is needed to allow large population (epidemiology) studies in order to verify current standards and, if necessary, formulate new guidelines for exposure. We designed an integrated instrument to detect volatile toxin in human samples for rapid biomonitoring that can measure Volatile Organic Compounds (VOCs) concentration in biological samples with a portable, low cost, deployable device, with the sensitivity and specificity similar to a bench top GC-MS (gold standard) system. In preliminary studies, we have demonstrated the novel concept of the device and its ability to accurately measure volatile organics in human plasma. The project milestone for successful completion is the demonstration of a statistically significant correlation of device readout with standard measures of GC-MS in a double-blind study. To achieve this goal, we will build upon our prototype device, which has shown significant sensitivity in rapidly detecting organic compounds in plasma, by implementing software, and hardware changes that would lead to sensitivities and specificities that would enable the measurement of trace biomonitor of VOCs. Successful completion of this work will allow us to proceed to more rigorous testing of the proposed device for ultimate use in biomonitor of VOCs and other toxins in a variety of human samples.

 描述（由适用提供）：生物监测量在人的生物学样本（例如血液或尿液）中测量化学物质及其代谢产物。它表明了人类对化学物质的接触量，包括但不限于空气，土壤，水，灰尘和食物，包括但不限于呼吸，触摸和摄入。通过国家生物监测计划（NBP）和国家健康与营养检查调查，CDC建立了黄金标准方法，以测量超过450种化学物质和营养标记物中的血液，血清，尿液，母乳和胎牛。但是，需要便携式和多功能系统来分析样品收集点上相似样品范围的多个分析物，以显着提高检测准确性。此外，还需要替代较低的COS方法来允许大量人群（流行病学）研究以验证当前标准，并在必要时制定新的暴露准则。我们设计了一种集成的仪器，以检测人类样品中的挥发性毒素，以快速生物监测，以测量具有便携式，低成本，可部署设备的生物样品中挥发性有机化合物（VOC）浓度，其敏感性和特异性类似于Bench TOP GC-MS（金标准）系统。在初步研究中，我们证明了该设备的新概念及其准确测量人血浆中挥发性有机物的能力。成功完成的项目里程碑是设备具有统计学意义相关性的演示 在一项双盲研究中，读取了GC-MS的标准测量。为了实现这一目标，我们将建立原型设备，该原型设备在快速检测血浆中的有机化合物，通过实现软件以及硬件变化而导致敏感性和特异性的硬件变化方面表现出显着的敏感性，从而能够测量VOC的痕量生物调子。成功完成这项工作将使我们能够对所提出的设备进行更严格的测试，以在各种人类样品中的VOC和其他毒素生物监测器中最终使用。