Molecular and Structural Biology

分子和结构生物学

基本信息

批准号：
8843937
负责人：
Bruce Furie
金额：
$ 19.56万
依托单位：
BETH ISRAEL DEACONESS MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至 2016-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8843937
关键词：
Blood Coagulation Factor Blood coagulation CD4 Positive T Lymphocytes Carbon Isotopes Cells Charge Complex Computer software Computers Crystallization Crystallography Docking Doctor of Philosophy Documentation Elements Frequencies Gel Hemostatic function High Pressure Liquid Chromatography Journals Leg Ligands Linux Lipase Methods Molecular Molecular Biology Molecular Cloning Molecular Evolution Monitor NMR Spectroscopy Nature Nitrogen Paper Peer Review Physiologic pulse Postdoctoral Fellow Process Protein Disulfide Isomerase Proteins Publishing Reader Recombinant Proteins Reporting Research Research Peer Review Robot Roentgen Rays Running Science Services Solutions Structural Biologist Structure Students System Systems Analysis Temperature Thrombosis Training Water Work X-Ray Crystallography data modeling design experience expression cloning instrumentation protein expression protein purification radio frequency research study skills structural biology vibration

项目摘要

The aim of this core is to provide technical support in molecular biology and structural biology to all projects. The structural biology facility has existed in the Division of Hemostasis and Thrombosis for more than a decade with the first paper on the NMR study of a blood coagulation protein published in 1996. Since then, the facility has reported on dozens of structures and key structural elements of coagulation factors. The three components of this core will be 1) NMR spectroscopy, 2) X-ray crystallography, and 3) molecular biology. The NMR spectroscopy component will be managed and operated by Dr. Natalia Beglova, the X-ray crystallography component by Mingdong Huang, and the molecular biology component by Lin Lin. Dr. Beglova is an experienced structural biologist. She combines solution NMR spectroscopy. X-ray crystallography and molecular docking approaches in her research published in peer-reviewed journals including Molecular Cell, Nature Structural Biology, PNAS and Structure. Dr. Huang is a senior X-ray crystallographer. He has solved dozens of protein crystal structure. He has trained 16 PhD students and postdoctoral fellows, and has published more than 60 peer reviewed research articles in journals including Science and Nature. He will be in charge of the administration of Core C and the work related to protein crystallography. Dr. Lin Lin is a senior research associate with extensive experience in both molecular biology, protein expression and structural biology. She has devised a molecular evolution method to optimize enzymatic activity of a lipase. This work shows her expertise in molecular biology. She will be in charge of the work related to protein cloning, expression and crystallization.

该核心的目的是为所有项目提供分子生物学和结构生物学的技术支持。结构生物学设施已经存在于止血和血栓形成的分裂中已有十多年了，其中的第一篇论文是关于1996年发表的血液凝血蛋白的NMR研究。此后，该设施报告了数十个结构和关键的结构性元素。三个组成部分该核心将是1）NMR光谱，2）X射线晶体学和3）分子生物学。 NMR光谱分量将由Natalia Beglova博士，Mingdong Huang的X射线晶体学成分以及Lin Lin的Molecular Biology Comports管理和操作。 Beglova博士是一位经验丰富的结构生物学家。她结合了溶液NMR光谱法。 X射线晶体学和分子对接她在同行评审期刊上发表的研究中的方法，包括分子细胞，自然结构生物学，PNA和结构。黄博士是一位高级X射线晶体学家。他已经解决了数十种蛋白质晶体结构。他已经培训了16名博士学位学生和博士后研究员，并出版了60多个同行回顾了包括科学和自然在内的期刊中的研究文章。他将负责核心C的给药以及与蛋白质晶体学有关的工作。 Lin Lin博士是一名高级研究助理，在分子生物学，蛋白质表达和结构生物学方面拥有丰富的经验。她设计了一个分子优化脂肪酶的酶活性的进化方法。这项工作显示了她在分子生物学方面的专业知识。她将负责与蛋白质克隆，表达和结晶有关的工作。