LCM Instrument, Arcturus XT-TI

LCM 仪器，Arcturus XT-TI

• 批准号: 8640630
• 负责人: Joel S Pachter
• 金额: $ 21.18万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-05-19 至 2015-05-18
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8640630
• 关键词: Advisory Committees; Attention; Bioinformatics; Biological; Capital; Cell Culture Techniques; Cells; Computer software; Computers; Connecticut; Core Facility; Development; Discipline; Ensure; Enzymes; Facility Designs; Faculty; Fee-for-Service Plans; Fluorescence; Foundations; Funding; Genetic Research; Genetic Transcription; Genomics; Growth; Human; Image; Immunofluorescence Immunologic; Infusion procedures; Investments; Ions; Lasers; Life; Link; Maine; Malignant Neoplasms; Microscope; Modeling; Molecular; Mus; Process; Program Development; Reagent; Research; Sampling; Seminal; Services; Signal Transduction; Source; Staining method; Stains; Technical Expertise; Technology; The Jackson Laboratory; Tissues; Training; Universities; base; experience; flexibility; instrument; meetings; operation; programs; tissue culture; ultraviolet

DESCRIPTION (provided by applicant): We are requesting a state of the art laser capture microscope (LCM) as the seminal instrument for an institutionally supported LCM core facility designed to service a broad spectrum of biological disciplines within the University of Connecticut. Our University is experiencing a major infusion of capital and operational funds from the State as part of the concept called Bioscience Connecticut. An immediate consequence of this investment was the decision by the Jackson Laboratories to establish a major research effort in the genomics of human cancer. This UCONN/JAX affiliation extends to their mouse genetic research programs located in Maine. To meet the increasing demands of this well-funded and broad user group, the instrument, Arcturus model XTTM, will be capable of immunofluorescence-based imaging that builds on the foundation for PCR analysis of laser captured samples developed by the core director, Dr. Joel Pachter. This particular model is unique among all LCM instruments currently available in having both infrared and ultraviolet laser capacities, providing users the greatest flexibility in cell acquisition. The technology willbe extended to capture cells within tissue or cell culture expressing GFP and a variety of fluorescence-based enzyme stains from which an extensive RNAseq analysis can be performed using an Ion Torrent platform. Capabilities for target capture based on multiplexing fluorescence signal and computer defined target selection will allow for flexible growth as new tissue sources are analyzed. The core will train users in the operation of the instrument and introduce them to a workflow that includes extensive RNA expression and bioinformatics characterization. The financial and compliance aspects of this fee for service core will be handled by existing reagent and common instrument core (Molecular Store) directed by the co-director of the LCM core (D. Rowe) using a server controlled software program that is easily adapted to activities of the LCM core. An experienced advisory committee has been established to ensure optimal utilization and attention to emerging applications of the instrument. In the process of implementing our LCM core concept, we will become a Life Technology Center of Excellence for our concept of linking LCM and RNAseq. This designation identifies us as a model for programs outside our University, and enables us to be involved in the development and implementation of new applications of the LCM platform. We have the fundamentals of institutional support, technical expertise, window to new applications, and the operational and financial experience in place to provide a heavily utilized and rapidly evolving core facility that will support even greater succes of our research faculty for funding and program development.

描述（由申请人提供）：我们要求使用最先进的激光捕获显微镜（LCM）作为机构支持的LCM核心设施的开创仪器，旨在为康涅狄格大学内的广泛生物学科服务。我们的大学正经历了该州的大量注入资本和运营基金，这是康涅狄格州生物科学的一部分。这项投资的直接结果是杰克逊实验室决定在人类癌症的基因组学上建立重大研究工作。该UCONN/JAX隶属关系扩展到其位于缅因州的小鼠遗传研究计划。为了满足这个资金丰富且广泛的用户组不断增长的需求，仪器Arcturus Model XTTM将能够基于免疫荧光成像，这是基于PCR分析的激光捕获的样品的基础，由核心主管Joel Pachter博士开发。在当前具有红外线和紫外线激光能力的所有LCM仪器中，这种特殊模型都是独一无二的，为用户提供了最大的细胞获取灵活性。该技术将扩展到在表达GFP的组织或细胞培养物中捕获细胞以及各种基于荧光的酶染色，可以使用ION Torrent平台进行广泛的RNASEQ分析。基于多路复用荧光信号和计算机定义的目标选择的目标捕获功能将允许随着新的组织源的分析。核心将培训用户进行仪器的操作，并将其介绍到包括广泛的RNA表达和生物信息学表征的工作流程。服务核心费用的财务和合规性方面将由LCM Core（D. Rowe）指导的现有试剂和共同仪器核心（Molecular Store）使用服务器控制的软件程序，该程序易于适应LCM Core的活动。已经成立了一个经验丰富的咨询委员会，以确保最佳利用和关注该工具的新兴应用。在实施LCM核心概念的过程中，我们将成为联系LCM和RNASEQ概念的卓越生活中心。该名称将我们确定为大学以外的计划的模型，并使我们能够参与LCM平台的新应用程序的开发和实施。我们拥有机构支持，技术专业知识，新应用程序窗口的基础，以及提供大量利用且迅速发展的核心设施的运营和财务经验，这些设施将支持我们的资金和计划发展研究学院的更大成功。