Electron Microscopy Core

电子显微镜核心

• 批准号: 9157591
• 负责人: NICO TJANDRA
• 金额: $ 2.26万
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9157591
• 关键词: Antigens; Carbon; Cell surface; Cells; Contractor; Core Facility; Cultured Cells; Cytoskeleton; Electron Microscope; Electron Microscopy; Image; Immunogold Technics; Light Microscope; Membrane; Membrane Lipids; Microtomy; Molecular Structure; National Heart, Lung, and Blood Institute; Negative Staining; Organ; Organism; Platinum; Polymers; Postdoctoral Fellow; Principal Investigator; Proteins; Publishing; Research; Resolution; Scanning; Scanning Electron Microscopy; Scientist; Services; Shadowing (Histology); Structure; Students; Techniques; Tissues; Training; Transmission Electron Microscopy; Vesicle; Work; digital imaging; immunocytochemistry; journal article; sample fixation; transmission process

The NHLBI Electron Microscopy Core Facility has supported projects using the following techniques in the past year: 1. Fixation, embedding, ultra-thin sectioning and transmission EM digital imaging of tissues and cells. 2. EM immunocytochemistry, including immunogold localization of proteins and other antigens within and on the surface of cells by pre-embedding and cryo-immunogold techniques. 3. Negative staining of large proteins, polymers and supramolecular structures as well as lipid and membrane vesicles for transmission EM imaging as well as platinum shadowing of large molecules. 4. Fixation, critical point drying, sputter-coating and scanning EM digital imaging of small organisms, organs, tissues and cells, as well as other materials such as artificial matrices. 5. Platinum carbon replicas of extracted, critical point dried cytoskeletons and the substrate-attached membranes of cultured cells In the past year, we have supported 45 projects for 27 principal investigators in the NHLBI/DIR and 6 project for a scientist outside the DIR. During this period, 14 journal articles have been published presenting from work in the EM Core with 12 where the EM Core Staff were co-authors or the EM Core was acknowledged. We have trained 14 postdoctoral fellows, students and contractors in the use of the scanning and transmission electron microscopes.

NHLBI电子显微镜核心设施在过去一年中使用以下技术支持了项目： 1。固定，嵌入，超薄切片和组织和细胞的传播EM数字成像。 2。EM免疫细胞化学，包括通过预插入和冷冻免疫质量技术在细胞内外的蛋白质和其他抗原的免疫原料定位。 3。大蛋白，聚合物和超分子结构以及用于传播EM成像的脂质和膜囊泡的负染色以及大分子的铂金阴影。 4。固定，临界点干燥，溅射涂层和扫描EM数字成像，对小生物，器官，组织和细胞以及其他材料，例如人造矩阵。 5。提取的，临界点干细胞骨架的铂碳复制品和培养细胞的底物附属膜 在过去的一年中，我们为NHLBI/DIR的27名主要研究人员和DIR以外的科学家的6个项目提供了45个项目。在此期间，已经在EM Core的工作中发表了14篇期刊文章，其中有12位，其中EM核心人员是合着者，或者承认EM Core。 我们已经培训了14名博士后研究员，学生和承包商的使用，用于使用扫描和传输电子显微镜。