Electron Microscopy Core

电子显微镜核心

• 批准号: 9157591
• 负责人: NICO TJANDRA
• 金额: $ 2.26万
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9157591
• 关键词: Antigens; Carbon; Cell surface; Cells; Contractor; Core Facility; Cultured Cells; Cytoskeleton; Electron Microscope; Electron Microscopy; Image; Immunogold Technics; Light Microscope; Membrane; Membrane Lipids; Microtomy; Molecular Structure; National Heart, Lung, and Blood Institute; Negative Staining; Organ; Organism; Platinum; Polymers; Postdoctoral Fellow; Principal Investigator; Proteins; Publishing; Research; Resolution; Scanning; Scanning Electron Microscopy; Scientist; Services; Shadowing (Histology); Structure; Students; Techniques; Tissues; Training; Transmission Electron Microscopy; Vesicle; Work; digital imaging; immunocytochemistry; journal article; sample fixation; transmission process

The NHLBI Electron Microscopy Core Facility has supported projects using the following techniques in the past year: 1. Fixation, embedding, ultra-thin sectioning and transmission EM digital imaging of tissues and cells. 2. EM immunocytochemistry, including immunogold localization of proteins and other antigens within and on the surface of cells by pre-embedding and cryo-immunogold techniques. 3. Negative staining of large proteins, polymers and supramolecular structures as well as lipid and membrane vesicles for transmission EM imaging as well as platinum shadowing of large molecules. 4. Fixation, critical point drying, sputter-coating and scanning EM digital imaging of small organisms, organs, tissues and cells, as well as other materials such as artificial matrices. 5. Platinum carbon replicas of extracted, critical point dried cytoskeletons and the substrate-attached membranes of cultured cells In the past year, we have supported 45 projects for 27 principal investigators in the NHLBI/DIR and 6 project for a scientist outside the DIR. During this period, 14 journal articles have been published presenting from work in the EM Core with 12 where the EM Core Staff were co-authors or the EM Core was acknowledged. We have trained 14 postdoctoral fellows, students and contractors in the use of the scanning and transmission electron microscopes.

NHLBI 电子显微镜核心设施在过去一年中支持了使用以下技术的项目： 1、组织、细胞的固定、包埋、超薄切片、透射电镜数字成像。 2. EM 免疫细胞化学，包括通过预包埋和冷冻免疫金技术对细胞内和细胞表面上的蛋白质和其他抗原进行免疫金定位。 3. 大蛋白质、聚合物和超分子结构以及脂质和膜囊泡的负染色，用于透射电镜成像以及大分子的铂阴影。 4.小型生物体、器官、组织和细胞以及人工基质等其他材料的固定、临界点干燥、溅射镀膜和扫描电镜数字成像。 5. 提取的、临界点干燥的细胞骨架和培养细胞的基质附着膜的铂碳复制品 去年，我们为 NHLBI/DIR 的 27 位主要研究人员支持了 45 个项目，为 DIR 之外的科学家支持了 6 个项目。在此期间，已发表 14 篇期刊文章，介绍 EM Core 的工作，其中 12 篇文章是 EM Core 工作人员共同作者或 EM Core 得到认可。 我们已经培训了 14 名博士后研究员、学生和承包商如何使用扫描和透射电子显微镜。