Malaria screening in resource-poor settings using a simple, power-free, cell phone-friendly device

使用简单、无电源、适合手机使用的设备在资源匮乏的环境中进行疟疾筛查

基本信息

批准号：
8925940
负责人：
IONITA Calin GHIRAN
金额：
$ 19.93万
依托单位：
BETH ISRAEL DEACONESS MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-09-10 至 2016-10-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8925940
关键词：
Acute Adult African Algorithms Anemia Antibodies Area Artemisinins Back Beds Bite Blood Blood Cells Blood Platelets Blood capillaries Blood specimen Buffers Burkitt Lymphoma Cell Separation Cell physiology Cellular Phone Cessation of life Child Child Mortality Chronic Code Collaborations Comorbidity Complex Complication Culicidae Custom Data Detection Devices Diagnosis Diagnostic Diagnostic Procedure Diagnostic tests Disease Drops Drug resistance Emergency treatment Equipment Erythrocytes Fatal Outcome Fever Geometry Glass Goals Gold Health Health Services Health system Heating Hematology Hemoglobin Hospitals Hour Human Resources Image Image Analysis Immune Immunity Individual Infection Insecticides Ions Laboratories Lead Letters Leukocytes Life Location Magnetism Malaria Malignant Neoplasms Malnutrition Measures Methods Microscope Microscopic Microscopy Nigeria Outcome Parasites Pathology Patients Pharmaceutical Preparations Plasmodium Plasmodium falciparum Plastics Pregnancy Prevalence Process Property Provider Public Health Reaction Reagent Recurrence Resources Risk Role Sensitivity and Specificity Shapes Sickle Cell Anemia Solutions Splenomegaly Staining method Stains Structure Symptoms System Technology Telephone Temperature Testing Time Training Universities Work Writing artemisinine base capillary cell type cloud based cost drug testing early childhood efficacy testing feeding hemozoin image processing improved innovation interest lens light microscopy light weight mortality novel pathogen prevent programs prototype research clinical testing screening stability testing tool vector mosquito

项目摘要

DESCRIPTION (provided by applicant): Malaria is a life-threatening disease caused by one the four species of Plasmodium parasites that are transmitted to people through the bites of infected mosquitoes. In 2012 alone, malaria caused over 625 000 deaths, mostly among African children. Due to its high child mortality and prevalence in Sub-Saharan regions, in 2000 a new program was launched in Nigeria, the Roll Back Malaria (RBM), aiming at reducing the burden of malaria by using presumptive treatment (PT) based, in many cases, only on fever as single sign of malaria infection. This approach, necessary due to limited laboratory support leading to long waiting times for laboratory diagnostic and consequently increased risks associated with delayed anti-malaria treatments, is often the only available option for local health service providers. Although studies have shown a significant decrease in malaria burden since the beginning of the RBM program in areas where the PT approach is employed, continuation of this practice already led to over-diagnosis of the malaria, under-diagnosis of other illness, increased drug wastage, increase adverse anti-malaria drug reactions, increased parasite-drug- resistance, and unnecessary financial burden to local health systems. We propose to develop an antibody- free diagnostic screening device that is based on the principles of magnetic levitation, allowing for separation of infected red blood cells from un-infected red blood cells. Our screening device will be light-weight, disposable, and cheap to manufacture with an indefinite shelf life at room temperature. The prototype system requires less than a drop of fingerprick blood (<1 �L) and a small volume of RBC-friendly buffer containing paramagnetic ions. Binary diagnostic results is obtained within a few minutes solely by using a set of permanent magnets immobilized in a plastic structure surrounding a glass/plastic capillary containing the blood. Results are recorded and stored using a standard camera phone. No additional imaging equipment, staining reagents, or consumables are required.

描述（由申请人提供）：疟疾是一种危及生命的疾病，由四种疟原虫寄生虫之一引起，通过受感染的蚊子叮咬传播给人类。仅 2012 年，疟疾就导致超过 625 000 人死亡，其中大部分是非洲儿童。由于撒哈拉以南地区的儿童死亡率和患病率较高，尼日利亚于 2000 年启动了一项新计划，即减少疟疾 (RBM)，旨在减少疟疾在许多情况下，仅将发烧作为疟疾感染的单一迹象，通过使用推定治疗（PT）来减轻疟疾的负担，这种方法是必要的，因为实验室支持有限，导致实验室诊断的等待时间较长，从而增加了与疟疾相关的风险。延迟抗疟疾治疗往往是当地卫生服务提供者唯一可用的选择，尽管研究表明，自 RBM 计划在采用 PT 方法的地区开始以来，疟疾负担显着下降，但这种做法的延续已经导致了疟疾负担的增加。过度诊断疟疾、其他疾病的诊断不足、药物浪费增加、抗疟疾药物不良反应增加、寄生虫耐药性增加以及给当地卫生系统带来不必要的经济负担。基于磁悬浮原理，可以将受感染的红细胞与未受感染的红细胞分离。我们的筛查设备重量轻、一次性且制造成本低廉，原型在室温下具有无限的保质期。系统需要少于一滴仅使用一组固定在玻璃/塑料毛细管周围的塑料结构中的永磁体，即可在几分钟内获得指尖血液（<1 µL）和少量含有顺磁性离子的适合红细胞的缓冲液。使用标准拍照手机记录和存储结果，无需额外的成像设备、染色试剂或耗材。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Sickle cell detection using a smartphone.

DOI：
10.1038/srep15022
发表时间：
2015-10-22
期刊：
Scientific reports
影响因子：
4.6
作者：
Knowlton SM;Sencan I;Aytar Y;Khoory J;Heeney MM;Ghiran IC;Tasoglu S
通讯作者：
Tasoglu S

Smart-Phone Based Magnetic Levitation for Measuring Densities.

DOI：
10.1371/journal.pone.0134400
发表时间：
2015
期刊：
PloS one
影响因子：
3.7
作者：
Knowlton S;Yu CH;Jain N;Ghiran IC;Tasoglu S
通讯作者：
Tasoglu S

Detection of membrane-bound and soluble antigens by magnetic levitation.

DOI：
10.1039/c7lc00402h
发表时间：
2017-10-11
期刊：
Lab on a chip
影响因子：
6.1
作者：
Andersen MS;Howard E;Lu S;Richard M;Gregory M;Ogembo G;Mazor O;Gorelik P;Shapiro NI;Sharda AV;Ghiran I
通讯作者：
Ghiran I