High Throughput Screening

高通量筛选

• 批准号: 8933496
• 负责人: HAKIM DJABALLAH
• 金额: $ 53.84万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8933496
• 关键词: Biological Assay; Biological Factors; Biological Process; Biology; Cancer Center Support Grant; Cancer cell line; Cells; Chemicals; Collection; Computer software; Core Facility; Custom; Data Analyses; Databases; Epidermal Growth Factor Receptor; Genes; Glycerol; Grant; Growth; Human; Individual; Industrialization; Institution; Instruction; Knowledge; Libraries; Malignant neoplasm of lung; Microscope; Mission; Mutation; Publications; RNA Interference; Reader; Research; Research Personnel; Research Support; Robotics; Screening for cancer; Services; Small Interfering RNA; Technology; Therapeutic Intervention; Validation; Work; assay development; base; data acquisition; data management; functional genomics; high throughput screening; instrumentation; mutant; novel; programs; screening; small hairpin RNA; small molecule; tool

The High-throughput Screening Core Facility (HTSCF) was established in 2003 to support the Institution's growth in chemical biology and functional genomics. The HTSCF's ongoing mission continues to support such efforts. Bioactive compounds are used as chemical tools to probe biological processes. The identification of novel molecules requires a broad range of tools including robust assays, large collections of chemicals, HTS technologies, and knowledge in hit validation and characterization steps. The HTSCF has modern robotics, custom built screening data management databases, chemical screening libraries, RNAi screening libraries, assay development and industrialization expertise, screening data analysis and management. The Core is equipped with two custom-built linear track robotic platforms harboring several dispensers, microtiter plate readers, automated microscopes among other instrumentation enabling both invitro target based and cell based assays to be routinely performed. Screening data acquisition and management is handled through a suite of custom built software. The compound library has grown to 400K chemicals; and contains a wide variety of natural products. The RNAi libraries have also grown to include both siRNA and shRNA capabilities covering 22K genes. Glycerol stocks of individual shRNA hairpins are provided as a service. One example of important work facilitated by this work was a screen against mutant EGFR in human lung cancer cell lines by the Varmus lab. The screening efforts led to the identification and characterization of four classes of small molecules overcoming the mutations. The broad range of services and collaborative work provided by the (HTSCF) has supported the research of 48 investigators in the past year. During the past grant period the work of the Core has contributed to 27 publications of researchers from 8 research programs.

高通量筛选核心设施 (HTSCF) 成立于 2003 年，旨在支持该机构的 化学生物学和功能基因组学的发展。 HTSCF 的持续使命是继续支持 这样的努力。生物活性化合物被用作探测生物过程的化学工具。这 新型分子的鉴定需要广泛的工具，包括强大的分析方法、大量的 化学品、高温超导技术以及命中验证和表征步骤方面的知识。 HTSCF 有 现代机器人技术、定制筛选数据管理数据库、化学筛选库、RNAi 筛选文库、分析开发和工业化专业知识、筛选数据分析和 管理。 Core 配备了两个定制的线性轨道机器人平台，其中包含多个 分配器、微量滴定板读数器、自动显微镜以及其他仪器，可实现体外 常规进行基于靶点和基于细胞的测定。筛选数据采集和 管理是通过一套定制软件进行的。化合物库已增长至400K 化学品；并含有多种天然产物。 RNAi 文库也已发展到包括 siRNA 和 shRNA 功能均覆盖 22K 基因。各个 shRNA 发夹的甘油储备是 作为服务提供。这项工作促进的重要工作的一个例子是针对突变体的筛选 Varmus 实验室在人类肺癌细胞系中的 EGFR。筛选工作导致了识别和 克服突变的四类小分子的表征。广泛的服务范围 (HTSCF) 提供的协作工作过去支持了 48 名研究人员的研究 年。在过去的资助期内，核心的工作为研究人员的 27 篇出版物做出了贡献 来自8个研究项目。