Biological Fate and Biocompatability of Dendritic and Silica-Based Nanoconstructs

树枝状和二氧化硅基纳米结构的生物命运和生物相容性

基本信息

批准号：
8761265
负责人：
Hamid Ghandehari
金额：
$ 33.53万
依托单位：
UNIVERSITY OF UTAH
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-28 至 2019-05-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8761265
关键词：
Acute Adverse event Affect Aftercare Amidone Amines Animal Experimentation Autophagocytosis Biodistribution Biological Biology Blood Platelets Cells Characteristics Charge Chronic Chronic Disease Clinical Clinical Trials Communities Cultured Cells Data Dendrimers Dendritic Cells Development Disease Dose Drug Kinetics Engineering Environment Evaluation Event Gene Expression Gene Expression Profiling Glycolates Goals Gold Grant Guidelines Human Immune Immune response In Vitro Infiltration Inflammation Inflammation Mediators Inflammatory Inflammatory Response Intracellular Transport Lead Lesion Liver Mediating Methods Morphology Organ Outcome Particulate Pathway interactions Phase Phenotype Porosity Preparation Process Property Proteins Reaction Safety Signal Transduction Silicon Dioxide Spleen Structure Surface Surface Properties System Tissues Toxic effect Toxicology Up-Regulation Work antimicrobial base coping mechanism density design in vivo innate immune function macrophage mitochondrial dysfunction nanomaterials nanoparticle nanorod neutrophil particle pathogen protein expression public health relevance response uptake

项目摘要

DESCRIPTION (provided by applicant): The global goal of this project is to assess how physicochemical characteristics of nanomaterials modulate biological environmental responses. In this application the influence of porosity, degradation and curvature of silica nanoparticles (SNPs) on mechanisms of cellular uptake, biodistribution, pharmacokinetics and toxicity will be examined. Specifically, we will examine in more detail the cellular fate and mechanisms of intracellular transport of the SNPs, how inflammatory responses are initiated in response to the changes in the physicochemical properties, and what would be the biological fate of degradation products of SNPs. Three Specific Aims are proposed: 1) 1. Synthesis and characterization of SNPs with systematic differences in size, porosity, surface roughness and functionality, and biodegradability, 2) Investigate how surface properties affect protein and cellular inflammatory mediated mechanisms in vitro, 3) Investigate chronic inflammatory mediated mechanisms as a function of surface properties in vivo.

描述（由申请人提供）：该项目的全球目标是评估纳米材料的物理化学特性如何调节生物环境反应。在此应用中，将检查二氧化硅纳米颗粒 (SNP) 的孔隙率、降解和曲率对细胞摄取、生物分布、药代动力学和毒性机制的影响。具体来说，我们将更详细地研究 SNP 的细胞命运和细胞内转运机制、如何响应理化性质的变化而引发炎症反应，以及 SNP 降解产物的生物学命运。提出了三个具体目标：1) 1. 合成和表征在尺寸、孔隙率、表面粗糙度和功能以及生物降解性方面具有系统差异的 SNP，2) 研究表面特性如何影响体外蛋白质和细胞炎症介导机制，3) 研究慢性炎症介导的机制作为体内表面特性的函数。