Assessing a Novel Paradigm of Inducing Cryotolerance

评估诱导低温耐受的新范式

• 批准号: 8441521
• 负责人: Ram Devireddy
• 金额: $ 17.85万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8441521
• 关键词: Adherence; Adipocytes; Adipose tissue; Alkaline Phosphatase; Allogenic; Animals; Antibodies; Autologous; Biochemical; Biological Assay; Cardiac; Cell Survival; Cell Therapy; Cells; Centrifugation; Chemicals; Chills; Chondrocytes; Clinical; Colony-Forming Units Assay; Colony-forming units; Communities; Cryopreservation; Cryopreserved Cell; Detection; Development; Digestion; Dimethyl Sulfoxide; Disease; Dyspnea; Effectiveness; Fibroblasts; Flow Cytometry; Freezing; Genes; Goals; HSPB1 gene; Heart; Heart Arrest; Heat shock proteins; Heat-Shock Response; Hormonal; Human; Hypotension; Hypoxia; Immunoblotting; Immunotherapy; In Vitro; Infusion procedures; Kidney Failure; Laboratories; Leptin; Link; Molecular Chaperones; Muscle Cells; Nausea; Neurologic; Neurons; Osteoblasts; Osteocalcin; Osteogenesis; Pathway interactions; Patients; Physicians; Plastics; Process; Protocols documentation; RNA analysis; Regimen; Relative (related person); Reporting; Reverse Transcriptase Polymerase Chain Reaction; Safety; Serum; Specimen; Stem cell transplant; Stem cells; Stimulus; Surface; Testing; Tissues; Toxic effect; Translating; Undifferentiated; Western Blotting; adiponectin; adult stem cell; base; bone sialoprotein; collagenase; conditioning; cost; improved; in vivo; lipid biosynthesis; mineralization; novel; oil red O; osteogenic; protein expression; research study; respiratory; response; standard of care

DESCRIPTION (provided by applicant):The practical goals of this study will be the development and optimization of a serum free cryoprotection medium with minimum levels of cryoprotectants. We intend to pursue these studies using a hypothesis-driven, mechanism-based experimental approach, i.e., the viability of the cryopreserved adult stem cells (ASCs), in the undifferentiated, adipocyte- or osteoblast- differentiated states, can be improved by increasing the pre-freeze expression of heat shock proteins (HSPs).

描述（由申请人提供）：本研究的实际目标是开发和优化具有最低水平冷冻保护剂的无血清冷冻保护介质。 我们打算使用假设驱动、基于机制的实验方法进行这些研究，即，可以通过增加未分化、脂肪细胞或成骨细胞分化状态的冷冻成体干细胞（ASC）的活力来提高热休克蛋白 (HSP) 的预冻表达。

项目成果

BIOPRESERVATION: HEAT/MASS TRANSFER CHALLENGES AND BIOCHEMICAL/GENETIC ADAPTATIONS IN BIOLOGICAL SYSTEMS.

生物保存：生物系统中的传热/传质挑战和生物化学/遗传适应。

• 发表时间: 2013
• 影响因子: 1.7
• 作者: Devireddy; Ram V
• 通讯作者: Ram V

Effects of Decade Long Freezing Storage on Adipose Derived Stem Cells Functionality.

长达十年的冷冻储存对脂肪干细胞功能的影响。

• 发表时间: 2018-05-25
• 影响因子: 4.6
• 作者: Shaik, Shahensha;Wu, Xiying;Gimble, Jeffrey;Devireddy, Ram
• 通讯作者: Devireddy, Ram

Single-Cell Analysis Using Hyperspectral Imaging Modalities.

使用高光谱成像方式进行单细胞分析。

• DOI: 10.1115/1.4038638
• 发表时间: 2018-02-01
• 期刊: Journal of biomechanical engineering
• 作者: Nishir Mehta;Shahensha Shaik;R. Devireddy;M. Gartia
• 通讯作者: M. Gartia

Inducing Heat Shock Proteins Enhances the Stemness of Frozen-Thawed Adipose Tissue-Derived Stem Cells.

诱导热休克蛋白增强冻融脂肪组织干细胞的干性。

• DOI: 10.1089/scd.2016.0289
• 发表时间: 2024-09-14
• 期刊: Stem cells and development
• 影响因子: 4
• 作者: Shahensha Shaik;Daniel Hayes;J. Gimble;R. Devireddy
• 通讯作者: R. Devireddy

microRNA Sequencing of CD34+ Sorted Adipose Stem Cells Undergoing Endotheliogenesis.

对正在进行内皮生成的 CD34 分选的脂肪干细胞进行 microRNA 测序。

• 发表时间: 2021
• 影响因子: 4
• 作者: Shaik, Shahensha;Martin, Elizabeth;Hayes, Daniel;Gimble, Jeffrey;Devireddy, Ram
• 通讯作者: Devireddy, Ram