Stress Reduction Studies in Mice and Humans

小鼠和人类的减压研究

基本信息

批准号：
8542758
负责人：
Blake Taylor Gurfein
金额：
$ 14.12万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-09-30 至 2017-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8542758
关键词：
Address Adrenal Glands Advocate Affect American Animal Experiments Animal Housing Animal Model Animals Anxiety Area Autonomic nervous system B-Lymphocytes Basic Science Behavior Bioinformatics Biological Biological Testing Biometry Boxing CD4 Positive T Lymphocytes CD8B1 gene Catecholamines Cell Count Cell physiology Cells Chronic Chronic Disease Chronic stress Clinical Clinical Research Communities Complementary and alternative medicine Control Animal Control Groups Coronary heart disease Corticosterone DNA Microarray Chip Data Development Disease Environment Equipment Exhibits Exposure to Funding Gene Expression Genes Glucocorticoids Goals Grant HIV Health Health Professional House mice Human Hypothalamic structure Immune Immune system Immunologics Individual Influenza Influenza vaccination Integrative Medicine Intervention Investigation Knowledge Laboratory Study Life Link Lymphocyte Lymphocyte Subset Malignant Neoplasms Measures Medical Medicine Mentors Methods Mind-Body Method Modeling Molecular Molecular Profiling Mus National Center for Complementary and Alternative Medicine Natural Killer Cells Nerve Degeneration Nervous system structure Nesting Behavior Neurosecretory Systems Outcome Paper Pathway interactions Physiological Pituitary Gland Plant Roots Principal Investigator Production Public Health Research Research Personnel Research Project Grants Resources Risk Role Sampling Scientist Shelter facility Signal Transduction Sorting - Cell Movement Spleen Stress T-Lymphocyte Techniques Testing Tissue-Specific Gene Expression Training Tube Vaccines Work Yoga adverse outcome biological systems career clinical practice density design experience human subject hypothalamic-pituitary-adrenal axis immune function infectious disease model lectures mind body interaction mindfulness-based stress reduction mouse model novel pathogen polycarbonate professor psychologic research study response tool

项目摘要

DESCRIPTION (provided by applicant): The purpose of this K01 Mentored Research Scientist Development application is to develop the candidate as a translational investigator focused on bridging areas of basic science and clinical research to accelerate mechanism-focused investigations of complementary and alternative medicine (CAM). This K01 will provide Dr. Gurfein with the support necessary to accomplish the following goals: (1) to further develop and validate a novel animal model of stress reduction that can be used to advance testing of biological pathways influenced by CAM-related stress reduction interventions; (2) to acquire training in clinical research, bioinformatics, and advanced biostatistics; (3) to gain pivotal experience designing and implementing clinical research (4) to gain expertise in cutting edge basic science techniques such as the application of DNA microarrays to study CAM interventions; and (5) to develop the resources necessary for an independent research career. To achieve these goals, Dr. Gurfein has assembled a mentoring team comprised of a primary mentor, Dr. Frederick Hecht, Professor of Medicine, Director of Research at the Osher Center for Integrative Medicine at UCSF, and principal investigator of a NCCAM funded P01 grant on mind-body interactions, and two co-mentors: Dr. Douglas Nixon, Professor of Medicine and Associate Chief of the Division of Experimental Medicine at UCSF, whose laboratory studies immune cell responses to stress and an array of pathogens; and Dr. Steve Cole, Associate Professor of Medicine at UCLA and an expert on socioenvironmental factors that affect gene expression. In addition to extensive mentoring, a carefully selected combination of didactic coursework, lectures, and research will provide Dr. Gurfein with the training and experience that he requires to transition to a role of fully independent investigator. UCSF is an ideal environment for the proposed training because it provides access to cutting edge facilities and equipment and to experts in clinical, basic science, and integrative medicine research. This training will be applied to conduct a research project that aims to further develop an animal model of stress reduction and apply it to identify and compare the immunologic and gene expression changes that result from stress reduction in mice and humans. Stress reduction in mice is achieved through altering the caging environment and by including enhancements that are designed to minimize mouse stress levels. The research addresses the following aims: To validate and further develop a mouse model that will be used to study the effects and biological mechanisms of stress reduction (Aim 1). Immune cell subsets from reduced-stress animals will be studied for changes in number, proportion, and function (Aim 2). Using DNA microarrays, sorted lymphocytes from reduced-stress mice and humans trained in mindfulness-based stress reduction will be assessed to identify similarities and differences in gene expression change (Aim 3). These aims build toward an R01 in which the mouse stress reduction model would be used to answer questions relevant to public health such as the effects of stress reduction interventions on influenza vaccination and infection.

描述（由申请人提供）：此 K01 指导研究科学家开发申请的目的是将候选人培养为转化研究者，专注于基础科学和临床研究的桥梁领域，以加速补充和替代医学 (CAM) 的机制研究。该 K01 将为 Gurfein 博士提供实现以下目标所需的支持：(1) 进一步开发和验证一种新颖的减压动物模型，该模型可用于推进受 CAM 相关减压干预措施影响的生物途径的测试; (2) 接受临床研究、生物信息学和高级生物统计学方面的培训； (3) 获得设计和实施临床研究的关键经验 (4) 获得尖端基础科学技术的专业知识，例如应用 DNA 微阵列来研究 CAM 干预措施； (5) 开发独立研究事业所需的资源。为了实现这些目标，Gurfein 博士组建了一个指导团队，由主要导师 Frederick Hecht 博士组成，他是加州大学旧金山分校奥舍尔综合医学中心的医学教授、研究主任，也是 NCCAM 资助的 P01 资助项目的首席研究员身心相互作用，以及两位共同导师：道格拉斯·尼克松博士，加州大学旧金山分校医学教授兼实验医学部副主任，他的实验室研究免疫细胞对压力和免疫细胞的反应。一系列病原体； Steve Cole 博士是加州大学洛杉矶分校医学副教授，也是影响基因表达的社会环境因素方面的专家。除了广泛的指导之外，精心挑选的教学课程、讲座和研究组合将为古尔芬博士提供过渡到完全独立研究者角色所需的培训和经验。 UCSF 是一个理想的环境对于拟议的培训，因为它提供了使用最先进的设施和设备以及临床、基础科学和综合医学研究专家的机会。此次培训将申请开展一项研究项目，旨在进一步开发减压动物模型，并将其应用于识别和比较小鼠和人类减压引起的免疫和基因表达变化。通过改变笼养环境和包括旨在最大限度地降低小鼠压力水平的增强措施来减轻小鼠的压力。该研究旨在实现以下目标：验证并进一步开发小鼠模型，用于研究减压的效果和生物机制（目标 1）。将研究来自压力减轻动物的免疫细胞亚群的数量、比例和功能的变化（目标 2）。使用 DNA 微阵列，对来自受过正念减压训练的减压小鼠和人类的分类淋巴细胞进行评估，以确定基因表达变化的相似性和差异（目标 3）。这些目标以 R01 为目标，其中小鼠减压模型将用于回答与公共卫生相关的问题，例如减压干预措施对流感疫苗接种和感染的影响。