Investigation of Biomarkers for Sugars Intake - A Controlled Feeding Study

糖摄入量生物标志物的研究 - 一项控制喂养研究

• 批准号: 8945307
• 负责人: Natasha Tasevska
• 金额: $ 49.26万
• 依托单位: ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-01 至 2020-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8945307
• 关键词: Adverse effects; Alanine; Alaska Native; Beverages; Biological; Biological Markers; Blood; Blood specimen; Calibration; Carbon; Cardiovascular Diseases; Chronic Disease; Collection; Confidence Intervals; Consumption; Dental caries; Development; Diet; Disease; Energy Intake; Epidemiologic Studies; Equation; Error Sources; Erythrocytes; Exhibits; Food; Fructose; Future; General Population; Guidelines; Health; Individual; Intake; Investigation; Life; Link; Malignant Neoplasms; Masks; Measurement; Measures; Modeling; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Participant; Patient Self-Report; Performance; Population Study; Prospective Studies; Records; Recovery; Resources; Risk Estimate; Sampling; Sampling Studies; Spottings; Sucrose; Sum; Sweetening Agents; Testing; Time; Total Sugar; Urine; Woman; Work; aged; base; cardiovascular disorder risk; disorder risk; feeding; men; metabolic abnormality assessment; novel strategies; protein intake; public health relevance; stable isotope; sugar; urinary; validation studies

 DESCRIPTION (provided by applicant): Apart from sugars' proven association with dental caries and obesity, the link to cardiovascular disease, type 2 diabetes and cancer remains largely inconclusive. Most population studies rely on self-reported measures of diet, known to be associated with large measurement error, which may be obscuring the true relationship between sugars and disease risk and may explain the lack of consistency in the observed evidence. Development of novel approaches for obtaining more accurate estimates of intake is crucial for attaining more reliable risk estimates for sugars and disease risk. Recently, 24-h urinary sucrose and fructose (24uSF) was developed as a biomarker of sugars intake in two UK-based feeding studies. However, evidence on the performance and the validity of this biomarker in U.S. participants and in the context of a U.S. diet is lacking. Further, the utility of uSF measure in spot urines has never been investigated and could have significant implications for epidemiologic studies. Another promising sugars biomarker, the carbon stable isotope ratio (d13C) in red blood cell (RBCs) has been recently developed among Alaska Natives. This biomarker has been tested against self-reports only, and has never been investigated in a feeding study with known intake. We now propose a 15-d highly-controlled feeding study to comprehensively investigate the performance of 24uSF and d13C as biomarkers of sugars intake, individually and in combination, and to inform future application of these promising biomarkers among U.S. participants. The study will involve 107 men and women aged 18-70 y, consuming only foods and beverages provided by the study metabolic kitchen for 15-d, that will mimic their usual diet, assessed previously by two 7-d food records. Over the 15-d feeding period, participants will collect 8 nonconsecutive 24-h urines, and blood samples will be collected at baseline, at study completion, and 5 weeks post-study completion. First we will investigate the performance of 24uSF as a biomarker of sugars intake and we will develop a calibration equation that will define the future application of 24uSF as an unbiased measure of sugars in U.S. participants. Further, we will investigate the utility of measuring uSF in spot urin as a biomarker of intake; on two 24-h urine collection days, participants will collect urine voids n separate containers. Second, we will investigate the performance of RBCs d13C as a biomarker of sugars intake among U.S. participants under controlled conditions of a feeding study. We will also explore the use of 24-h urinary d13C as a measure of sugars intake. Third, we will investigate approaches of combining 24uSF and d13C in a unique measure. Combining these two unrelated biomarkers with different sources of error and with different time-relation to intake may provide a superior measure of intake. This study will develop validated sugars biomarkers that can be used as unbiased measures of sugars intake among U.S. individuals that will ultimately allow obtaining reliable risk estimates of sugars-disease associations. Until strong and consistent evidence for adverse health effects of sugars is found, no firm guidance can be given to the general public.

 描述（由适用提供）：除了糖与龋齿和肥胖的可靠关联外，与心血管疾病的联系，2型糖尿病和癌症的联系在很大程度上仍然尚无定论。大多数人群研究依赖于自我报告的饮食测量，已知与大量测量误差有关，这可能掩盖了糖与疾病风险之间的真实关系，并且可能解释了观察到的证据中缺乏一致性。开发新的方法来获得更准确的摄入量估计，对于对糖和疾病风险进行更可靠的风险估计至关重要。最近，在两项基于英国的进食研究中，开发了24小时的尿蔗糖和果糖（24USF）作为糖摄入的生物标志物。但是，缺乏有关该生物标志物在美国参与者和美国饮食背景下的性能和有效性的证据。此外，从未研究过USF测量在尿液中的效用，并且可能对流行病学研究产生重大影响。另一个承诺糖生物标志物是红细胞（RBC）中的碳稳定同位素比（D13C）最近在阿拉斯加土著人中开发的。该生物标志物仅针对自我报告进行了测试，并且从未在一项已知摄入量的喂养研究中进行调查。现在，我们提出了一项15D高度控制的喂养研究，以全面研究24USF和D13C作为糖摄入糖的生物标志物，单独和组合的生物标志物，并为这些承诺的生物标志物在美国参与者中的未来应用提供信息。这项研究将涉及107名18-70岁的男女，仅食用由研究代谢厨房提供的15-D的食物和勇气，这将模仿他们通常的饮食，以前通过两种7-D食品记录进行了评估。在15D喂养期间，参与者将收集8次非连续的24小时尿液，并将在基线，研究完成和研究后完成5周收集血液样本。首先，我们将研究24USF作为糖摄入量的生物标志物的性能，我们将开发一个校准方程，该方程将定义24USF的未来应用，作为对美国参与者中糖的公正测量。此外，我们将调查在尿液中测量USF作为摄入量的生物标志物的实用性；在两个24小时的尿液收集天数，参与者将收集尿液空隙n单独的容器。其次，我们将研究RBCS D13C作为喂养研究的受控条件下的美国参与者中糖摄入量的生物标志物的表现。我们还将探索使用24小时尿D13C作为糖摄入量的量度。第三，我们将研究将24USF和D13C组合在独特测量中的方法。将这两个无关的生物标志物与不同的误差源和不同的时间融合结合在一起 可以提供较高的摄入量测量。这项研究将开发经过验证的糖生物标志物，这些生物标志物可以用作美国个体中糖摄入量的无偏测量，这些测量最终将允许获得可靠的糖 - 糖尿病关联风险估计。直到坚强 发现糖的不良健康影响的一致证据，没有向公众提供任何牢固的指导。