Investigation of Biomarkers for Sugars Intake - A Controlled Feeding Study

糖摄入量生物标志物的研究 - 一项控制喂养研究

• 批准号: 8945307
• 负责人: Natasha Tasevska
• 金额: $ 49.26万
• 依托单位: ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-01 至 2020-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8945307
• 关键词: Adverse effects; Alanine; Alaska Native; Beverages; Biological; Biological Markers; Blood; Blood specimen; Calibration; Carbon; Cardiovascular Diseases; Chronic Disease; Collection; Confidence Intervals; Consumption; Dental caries; Development; Diet; Disease; Energy Intake; Epidemiologic Studies; Equation; Error Sources; Erythrocytes; Exhibits; Food; Fructose; Future; General Population; Guidelines; Health; Individual; Intake; Investigation; Life; Link; Malignant Neoplasms; Masks; Measurement; Measures; Modeling; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Participant; Patient Self-Report; Performance; Population Study; Prospective Studies; Records; Recovery; Resources; Risk Estimate; Sampling; Sampling Studies; Spottings; Sucrose; Sum; Sweetening Agents; Testing; Time; Total Sugar; Urine; Woman; Work; aged; base; cardiovascular disorder risk; disorder risk; feeding; men; metabolic abnormality assessment; novel strategies; protein intake; public health relevance; stable isotope; sugar; urinary; validation studies

 DESCRIPTION (provided by applicant): Apart from sugars' proven association with dental caries and obesity, the link to cardiovascular disease, type 2 diabetes and cancer remains largely inconclusive. Most population studies rely on self-reported measures of diet, known to be associated with large measurement error, which may be obscuring the true relationship between sugars and disease risk and may explain the lack of consistency in the observed evidence. Development of novel approaches for obtaining more accurate estimates of intake is crucial for attaining more reliable risk estimates for sugars and disease risk. Recently, 24-h urinary sucrose and fructose (24uSF) was developed as a biomarker of sugars intake in two UK-based feeding studies. However, evidence on the performance and the validity of this biomarker in U.S. participants and in the context of a U.S. diet is lacking. Further, the utility of uSF measure in spot urines has never been investigated and could have significant implications for epidemiologic studies. Another promising sugars biomarker, the carbon stable isotope ratio (d13C) in red blood cell (RBCs) has been recently developed among Alaska Natives. This biomarker has been tested against self-reports only, and has never been investigated in a feeding study with known intake. We now propose a 15-d highly-controlled feeding study to comprehensively investigate the performance of 24uSF and d13C as biomarkers of sugars intake, individually and in combination, and to inform future application of these promising biomarkers among U.S. participants. The study will involve 107 men and women aged 18-70 y, consuming only foods and beverages provided by the study metabolic kitchen for 15-d, that will mimic their usual diet, assessed previously by two 7-d food records. Over the 15-d feeding period, participants will collect 8 nonconsecutive 24-h urines, and blood samples will be collected at baseline, at study completion, and 5 weeks post-study completion. First we will investigate the performance of 24uSF as a biomarker of sugars intake and we will develop a calibration equation that will define the future application of 24uSF as an unbiased measure of sugars in U.S. participants. Further, we will investigate the utility of measuring uSF in spot urin as a biomarker of intake; on two 24-h urine collection days, participants will collect urine voids n separate containers. Second, we will investigate the performance of RBCs d13C as a biomarker of sugars intake among U.S. participants under controlled conditions of a feeding study. We will also explore the use of 24-h urinary d13C as a measure of sugars intake. Third, we will investigate approaches of combining 24uSF and d13C in a unique measure. Combining these two unrelated biomarkers with different sources of error and with different time-relation to intake may provide a superior measure of intake. This study will develop validated sugars biomarkers that can be used as unbiased measures of sugars intake among U.S. individuals that will ultimately allow obtaining reliable risk estimates of sugars-disease associations. Until strong and consistent evidence for adverse health effects of sugars is found, no firm guidance can be given to the general public.

 描述（由申请人提供）：除了糖已被证实与龋齿和肥胖相关外，糖与心血管疾病、2 型糖尿病和癌症的联系在很大程度上仍然没有定论，大多数人口研究依赖于自我报告的饮食测量，已知这些测量与饮食相关。测量误差较大，这可能会掩盖糖与疾病风险之间的真实关系，并可能解释观察到的证据缺乏一致性，开发获得更准确的摄入量估计值的新方法对于获得更可靠的糖风险估计至关重要。最近，在两项基于英国的喂养研究中，24 小时尿蔗糖和果糖 (24uSF) 被开发为糖摄入量的生物标志物。该生物标志物在美国参与者和美国饮食背景下的性能和有效性的证据此外，uSF 测量在尿液中的效用从未被研究过，并且可能对另一种有前景的糖生物标志物——碳稳定性——产生重大影响。最近在阿拉斯加原住民中开发了红细胞 (RBC) 中的同位素比率 (d13C)，该生物标记仅针对自我报告进行了测试，并且从未在已知摄入量的喂养研究中进行过研究。 d 高度控制的喂养研究，以全面研究 24uSF 和 d13C 作为糖摄入生物标志物（单独或组合）的性能，并为这些有前途的生物标志物在美国参与者中的未来应用提供信息。将涉及 107 名年龄在 18-70 岁之间的男性和女性，在 15 天内仅食用由研究代谢厨房提供的食物和饮料，这将模仿他们的日常饮食，之前通过 15 天内的两个 7 天食物记录进行评估。在喂养期间，参与者将收集 8 次非连续 24 小时尿液，并在基线、研究完成时和研究完成后 5 周收集血液样本。 24uSF 作为糖摄入量的生物标志物，我们将开发一个校准方程，该方程将定义 24uSF 作为美国参与者中糖分的无偏测量的未来应用。此外，我们将研究在现场尿液中测量 uSF 作为摄入量生物标志物的效用。在两个 24 小时尿液收集日，参与者将在不同的容器中收集尿液。其次，我们将研究红细胞 d13C 作为糖摄入量生物标志物的性能。我们还将探索使用 24 小时尿 d13C 来衡量糖摄入量的美国参与者。第三，我们将研究将 24uSF 和 d13C 结合起来的独特方法。具有不同的误差源和与摄入量不同的时间关系 这项研究将开发经过验证的糖类生物标志物，可用作美国个人糖类摄入量的公正衡量标准，最终可以可靠地获得糖类与疾病关联的风险估计。 虽然已经找到了糖对健康不利影响的一致证据，但无法向公众提供明确的指导。