Funtion of Uncharacterized Genes of Acinetobacter baumanii

鲍曼不动杆菌未表征基因的功能

• 批准号: 8688145
• 负责人: Samuel I Miller
• 金额: $ 161.53万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-24 至 2018-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8688145
• 关键词: Acinetobacter; Acinetobacter baumannii; Antibiotic Resistance; Antibiotics; Antimicrobial Resistance; Bacteria; Biochemical; Biocide; Cell Membrane Permeability; Cell surface; Code; Communicable Diseases; Data; Desiccation; Development; Disinfectants; Disinfection; Drug Targeting; Drug resistance; Enzymes; Genes; Genetic; Genome; Genomics; Goals; Hospitals; Individual; Infection; Knowledge; Modeling; Molecular; Multi-Drug Resistance; Natural Immunity; Nosocomial Infections; Opportunistic Infections; Organism; Pathogenesis; Pharmaceutical Preparations; Phenotype; Process; Proteins; Proteomics; Research; Research Proposals; Resistance; Resources; Role; Surface Properties; Technology; Testing; Therapeutic; Work; antimicrobial; base; biological adaptation to stress; clinically relevant; data management; efflux pump; environmental change; gene function; genetic analysis; human disease; mutant; novel strategies; pathogen; programs; public health relevance; relational database; response; screening; trait

DESCRIPTION (provided by applicant): Acinetobacter baumannii (Ab) is an emerging pathogen that causes primary and opportunistic infections, and is a serious concern in the hospital and battlefield setting. This organism has significant intrinsic resistance to antibioticsas well as an ability to quickly acquire new resistance. Additionally, Ab can persist in a desiccated state on fomites, making disinfection and management of nosocomial infections particularly troublesome. The goal of this research proposal is to find unexploited therapeutic approaches by elucidating the role of uncharacterized genes of Ab in this organism's mechanisms of pathogenesis, resistance, and persistence. Three projects will be established to 1) use a genomic approach to broadly identify the role of uncharacterized genes (Colin Manoil, Project 1 Director) 2) determine the role of envelope integrity with regard to bacterial viability (Sam Mille, PI/PD, Project 2 Director), and use expression analysis to identify genes and sRNAs of unknown function associated with drug, disinfectant, and desiccation tolerance (Carrie Harwood, Project 3 Director). The work of the Center will be supported by an Administrative Core (Core 1, John Kemner Core Leader), a Protein Interaction Technology Core (Jim Bruce Core 2 Director Leader), and a Data Dissemination/Resource Management Core (Mitch Brittnacher, Core 3 Director).

描述（由申请人提供）：鲍曼尼（AB）杆菌（AB）是一种新兴的病原体，会引起原发性和机会性感染，并且在医院和战场环境中是一个严重的关注点。该生物具有对抗生素的明显固有耐药性以及快速获得新抗性的能力。此外，AB可以坚持在Fomites上保持干燥状态，从而使医院感染的消毒和管理特别麻烦。 这项研究建议的目的是通过阐明AB在该生物体的发病机理，抗性和持久性机制中的作用来找到未开发的治疗方法。 Three projects will be established to 1) use a genomic approach to broadly identify the role of uncharacterized genes (Colin Manoil, Project 1 Director) 2) determine the role of envelope integrity with regard to bacterial viability (Sam Mille, PI/PD, Project 2 Director), and use expression analysis to identify genes and sRNAs of unknown function associated with drug, disinfectant, and desiccation tolerance (Carrie Harwood, Project 3 导演）。该中心的工作将得到行政核心（Core 1，John Kemner Core Leader），蛋白质相互作用技术核心（Jim Bruce Core 2主管领导者）和数据传播/资源管理核心（Mitch Brittnacher，Core 3 Director）的支持。