Virus like particles as carriers for carbohydrate based anti-Salmonella vaccines

病毒样颗粒作为碳水化合物抗沙门氏菌疫苗的载体

基本信息

批准号：
8823965
负责人：
Xuefei Huang
金额：
$ 7.68万
依托单位：
MICHIGAN STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-08-01 至 2017-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8823965
关键词：
Abbreviations Alkynes Amides Antibody Response Antigens Azides Bacteria Bacteriophages Bone Marrow Bovine Serum Albumin CD4 Positive T Lymphocytes Carbohydrates Cell surface Characteristics Chemistry Clinic Copper CpG dinucleotide Dendritic Cells Development Disease Dose Enzyme-Linked Immunosorbent Assay Enzymes Esters Galactose Glycoconjugates Granulocyte-Macrophage Colony-Stimulating Factor Helper-Inducer T-Lymphocyte Horseradish Peroxidase Immune response Immunoglobulin G Immunologic Memory Immunology Immunosorbents India Interleukin-4 Investigation Keyhole Limpet Hemocyanin Lead Length Link Lipid A Lipopolysaccharides Mannose Multi-Drug Resistance Mus Oligosaccharides Pattern Polysaccharides Public Health Reaction Receptors, Antigen, B-Cell Research Research Project Grants Resort Rhamnose Route Salmonella Salmonella Vaccines Salmonella enteritidis Salmonella infections Salmonella paratyphi Salmonella typhi Salmonella typhimurium Spottings Structure Synthesis Chemistry T-Cell Activation Tetanus Toxoid Time Toll-like receptors Tumor-Associated Carbohydrate Antigens Typhoid Fever Ursidae Family Vaccination Vaccine Adjuvant Vaccines Vi capsular polysaccharide Virus-like particle Work base carboxyfluorescein combat cycloaddition design enzyme linked immunospot assay man novel novel vaccines pathogenic bacteria prevent programs public health relevance resistant strain response vaccine development

项目摘要

DESCRIPTION: Salmonella infections (salmonellosis) are a major public health problem throughout the world. In response to RFA-13-179, Indo-U.S. Vaccine Action Program Small Research Grant Program (R03), a collaborative team is formed between two glycoscience groups in the US and India to develop carbohydrate based anti-Salmonella vaccines. Instead of relying on isolating polysaccharides from the pathogenic bacteria, in aim 1, synthetic strategies will be developed to produce structure well defined Salmonella associated glycans from major pathogenic serovars. These glycans will be conjugated with a virus like particle, bacteriophage Q? via state of the art bioconjugation chemistry. In aim 2, the humoral immune responses against these glycoconjugates will be evaluated. As the virus like particle Q? can display the carbohydrate antigens in a highly organized manner and elicit powerful helper T cell activation, it is expected that Q? conjugates can significantly boost the IgG antibody responses against the Salmonella associate glycans and generate glycan specific immunological memory. The abilities of the vaccine constructs to protect against lethal Salmonella challenge will be established. This work can lead to an exciting new direction for anti-Salmonella vaccine development.

描述：沙门氏菌感染（沙门氏菌病）是世界上一个主要的公共卫生问题。响应RFA-13-179，Indo-U.S。疫苗行动计划小型研究赠款计划（R03），在美国和印度的两个Glycoscience小组之间成立了一个合作团队，以开发基于碳水化合物的抗盐酸疫苗。在AIM 1中，将开发合成策略，而不是依靠从致病细菌中分离多糖，而是从主要致病性血清群中产生结构良好的沙门氏菌相关的聚糖。这些聚糖将与诸如颗粒，噬菌体Q之类的病毒结合？通过最先进的生物结合化学。在AIM 2中，将评估针对这些糖缀合物的体液免疫反应。作为粒子Q的病毒？可以以高度组织的方式显示碳水化合物抗原并引起强大的辅助T细胞激活，可以预期Q吗？缀合物可以显着增强针对沙门氏菌副聚糖的IgG抗体反应，并产生聚糖特定的免疫记忆。将建立疫苗构建物防止致命沙门氏菌挑战的能力。这项工作可能会导致抗盐酸疫苗开发的新方向。